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Plasmid#182880PurposeLentivirus for expression of Plexin-B2 locked ring mutantDepositorInserthPLXNB2 (PLXNB2 Human)
UseLentiviralTagsExpressionMutationchanged Isoleucine 436 to Cysteine and Threonine …PromoterAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLT3REVIR_MARK3 2573
Plasmid#107233PurposeshRNA 2573. Do not use Evos or Acurri on these cells. Assess by FACS specifically with Venus and DsRed lazers. Tet-ON-all-in-oneDepositorInsertMARK3 shRNA (22 nt) (MARK3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLT3REVIR_MARK3 1122
Plasmid#107232PurposeshRNA 1122. Do not use Evos or Acurri on these cells. Assess by FACS specifically with Venus and DsRed lazers. Tet-ON-all-in-oneDepositorInsertMARK3 shRNA (22 nt) (MARK3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
P3-dCas9-Tet1-GFP-Puro
Plasmid#190728PurposeExpression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)DepositorInsertdCas9-tet1
UseCRISPRTags3XFLAG and SV40 NLSExpressionMammalianMutationChanged Pro434 codon from CCG to CCC and Gly468 f…PromoterpCAGAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4-eIF3B
Plasmid#133308PurposeExpression of luciferase driven by eIF3B promoter regionDepositorInserteIF3B promoter (EIF3B Human)
UseLuciferaseTagsExpressionMammalianMutationPromotereIF3BAvailable sinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-30a (+)_CDK2-7 chimera (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)
Plasmid#217014PurposeExpression of CDK2-7 chimera in E.coliDepositorUseTagsC3 protease cleavable GST tagExpressionBacterialMutationCDK2 (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)PromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4-eIF3B MBE3 mutant
Plasmid#133310PurposeExpression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 3DepositorInserteIF3B promoter MBE3 mutant (EIF3B Human)
UseLuciferaseTagsExpressionMammalianMutationccacgtgacc changed to cAaAAAAaccPromotereIF3BAvailable sinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4-eIF3B MBE2 mutant
Plasmid#133309PurposeExpression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 2DepositorInserteIF3B promoter MBE2 mutant (EIF3B Human)
UseLuciferaseTagsExpressionMammalianMutationgccacatgcacc changed to gcAaAaAAcaccPromotereIF3BAvailable sinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
bActin-RSG-BiSKi
Plasmid#26437DepositorInsertbActin promoter
Use4726TagsExpressionMutationPromoterAvailable sinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGLU_64
Plasmid#225767PurposeThis is the native promoter from the ermC erythromycin resistance cassette.DepositorInsertPermC_[abr]
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Turquoise1-78-Venus
Plasmid#139372PurposeFRET StandardDepositorInsertTurquoise1 78AA Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICSL11024 (pICH47732::NOSp-NPTII-OCST)
Plasmid#51144PurposeConfers KAN resistance in plantaDepositorInsertNOSp-NPTII-OCST
UseTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Smad7
Plasmid#80893Purposemammalian expression of Smad7DepositorInsertSmad7 (Smad7 Mouse)
UseTagsFlagExpressionMammalianMutationaa 2-426PromoterCMVAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Smad7-N
Plasmid#80894Purposemammalian expression of Smad7 N terminal domainDepositorInsertSMAD family member 7 (Smad7 Mouse)
UseTagsFlagExpressionMammalianMutationaa 2-259PromoterCMVAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRosettav2
Plasmid#115557PurposeTo determine correct antibiotic selection conditions in mammalian cells; standard for titering experimentsDepositorInsertHygromycin-Blasticidin-Puromycin-EGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pUC-tracrRNA-PMtU6-26
Plasmid#209191PurposeCloning of sgRNAs for expression in plantsDepositorInsertMtU6-26 SnRNA promoter
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-tracrRNA-PMtU6-1
Plasmid#209190PurposeCloning of sgRNAs for expression in plantsDepositorInsertMtU6-1 SnRNA promoter
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Smad7-C
Plasmid#80895Purposemammalian expression of Smad7 C terminal domainDepositorInsertSmad7 (Smad7 Mouse)
UseTagsFlagExpressionMammalianMutationaa 206-426PromoterCMVAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Smad7-delta
Plasmid#80897Purposemammalian expression of Smad7 deltaDepositorInsertSmad7 (Smad7 Mouse)
UseTagsFlagExpressionMammalianMutationaa 2-409PromoterCMVAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only