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Plasmid#146604PurposeInsect Expression of DmNot1_1-1148DepositorInsertDmNot1_1-1148 (Not1 Fly)
UseTagsExpressionInsectMutation4 silent and two non silent N605S, K759M mutation…PromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3_102-330-V5His6_D
Plasmid#146129PurposeInsect Expression of DmEDC3_102-330DepositorInsertDmEDC3_102-330 (Edc3 Fly)
UseTagsExpressionInsectMutationone silent mutation A456G and three mutations N16…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsNot4_1-274_AG
Plasmid#148750PurposeMammalian Expression of HsNot4_1-274DepositorInsertHsNot4_1-274 (CNOT4 Human)
UseTagsExpressionMammalianMutationone silent mutation compared to the sequence give…PromoterAvailable sinceNov. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(T50A)
Plasmid#112727PurposeLav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.DepositorInsert6His-TEV-TBP6.7(T50A)
UseTags6His-TEVExpressionBacterialMutationT50APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
PatoM-OG241
Plasmid#72842PurposeInduced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. OG241 backbone.DepositorInsertPatoM
UseTagsExpressionBacterialMutationPromoterPatoMAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 c-SRC (WT)
Plasmid#42202DepositorInsertc-SRC (SRC Human)
UseTagsExpressionMammalianMutationL176F mutation compared to reference sequence NM_…PromoterCMVAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1
Plasmid#193942Purposea fully murine CAR targeting CD105 (endoglin), a component of the TGFβ receptor expressed on the surface of certain solid tumors and acute leukemias.DepositorInsertheavy- and light-chain regions of the MJ7/18 hybridoma and constructed a single chain variable fragment (scFv)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc-C Med12His
Plasmid#49240Purposeexpresses human Med12 with His tag in insect cellsDepositorInsertMed12 (MED12 Human)
UseTagsHisExpressionInsectMutationPromoterPolyhedrinAvailable sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
RP1B FUS 1-163
Plasmid#127192PurposeProtein expression for affinity purificationDepositorInsertFUS 1-163 (FUS Human)
UseTagshexahistidineExpressionBacterialMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
MBP-FUS_LC_WT
Plasmid#98653PurposeExpresses MBP-tagged FUS AA 1-163DepositorInsertFUS (FUS Human)
UseTags6xHis-MBPExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
MCP-eN3x9
Plasmid#210706PurposeThis plasmid express EF1a promoter driven MCP-eN3x9 transactivation moduleDepositorInsertMCP fused to transactivation domain comprising eNRF2 and 3x 9aa Transactivation domain
UseLentiviralTagsEGFPExpressionMammalianMutationN55K in MCPPromoterEF1aAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
Str-STIM1-NN_neomycin
Plasmid#65311PurposeLuminal ER hook only (RUSH system)DepositorInsertSTIM1 mutated for binding motif to microtubule (STIM1 Human)
UseTagsstreptavidinExpressionMammalianMutation2 amino acids of STIM1 (IP from SxIP motif) were …PromoterCMVAvailable sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
p2R3a-Tq2CFP-OcsT
Plasmid#71268PurposeEntry clone containing Turqoise2. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertTq2CFP
UseGatewayTags4xGly linker and octaline synthase terminatorExpressionMutationPromoterAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-CMV-XRCC1-EGFP-Hygro
Plasmid#176062PurposeEGFP fused to the C-terminus of XRCC1 & a hygromycin resistance cassetteDepositorInsertX-ray repair cross complementing 1 (XRCC1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6.2EmGFP-cDAXX
Plasmid#169727PurposeMammalian expression construct of GFP tagged cDAXXDepositorInsertcDAXX (DAXX Human)
UseTagsGFPExpressionMammalianMutationComprises residues 495-740PromoterCMVAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
MBP-FUS_LC_12E
Plasmid#98654PurposeExpresses MBP-tagged phosphomimetic FUS AA 1-163DepositorInsertFUS (FUS Human)
UseTags6xHis-MBPExpressionBacterialMutation12 S/T to EPromoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1-V1251R_R
Plasmid#147376PurposeMammalian Expression of HsNot1iso1-V1251RDepositorInsertHsNot1iso1-V1251R (CNOT1 Human)
UseTagsExpressionMammalianMutationA deletion of 5 AA 822-827 SKMKPS-> T and one …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
MBP-FUS_FL_12E
Plasmid#98652PurposeExpresses MBP-tagged full length phosphomimetic FUSDepositorInsertFUS (FUS Human)
UseTags6xHis-MBPExpressionBacterialMutation12 S/T to EPromoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 Pnos:Luciferase:Tnos-SF-35S:Renilla:Tnos-35S:P19:Tnos-SF (GB1116)
Plasmid#68218PurposeModule composed of 3 transcriptional units: Luciferase and Renilla proteins, and the Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expressionDepositorInsertLuc / Renilla / P19
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnos / 35S / 35SAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Str-STIM1-NN_puromycin
Plasmid#65310PurposeLuminal ER hook only (RUSH system)DepositorInsertSTIM1 mutated for binding motif to microtubule (STIM1 Human)
UseTagsstreptavidinExpressionMammalianMutation2 amino acids of STIM1 (IP from SxIP motif) were …PromoterCMVAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only