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Plasmid#154911Purpose1-621 amino acids of Arabidopsis PHYB (as in construct Addgene ID 154910) with the CAAX membrane moiety tag. Tyrosine residue 276 of PHYB was mutated to Histidine.DepositorInsertPhytochrome B (PHYB Mustard Weed)
UseXenopus/avian/zebrafishTagsCAAX membrane moiety and mCherryExpressionMammalianMutation1-621 amino acids of Arabidopsis PHYB, Tyrosine r…PromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCK072 p5E 147bp exorh:EGFP
Plasmid#195950PurposeGateway p5E vector with reporterDepositorInsertexorh minimal regulatory elements and EGFP
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-mKate2
Plasmid#162624PurposeAllows for transcription of mKate2 control for injection into zebrafish embryos for BiFC assaysDepositorInsertmKate2
UseTagsExpressionMammalianMutationPromoterSP6Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-Avi-Cerulean-RanGAP
Plasmid#79878PurposeBeta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
UseTagsAviExpressionBacterialMutationPromoterBeta-actin2Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
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actc1b-mKate2-SERCA1
Plasmid#109498PurposeMuscle specific zebrafish expression of mKate2 fused to sarcoplasmic/endoplasmic calcium ATPase 1DepositorInsertSERCA1 (atp2a1 Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPlease see Depositor CommentsPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
FnoCas12a-2C-NLS in pCSDest
Plasmid#126639PurposeExpresses FnoCas12a-2C-NLS in mammalian cellsDepositorInsertFnoCas12a-2C-NLS
UseCRISPRTags3xHuman influenza hemagglutinin (HA)-TagExpressionMammalianMutationPromoterCMV IE94 promoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
AsCas12a-2C-NLS in pCSDest
Plasmid#126637PurposeExpresses AsCas12a-2C-NLS in mammalian cellsDepositorInsertAsCas12a-2C-NLS
UseCRISPRTags3xHuman influenza hemagglutinin (HA)-TagExpressionMammalianMutationPromoterCMV IE94 promoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
CiDr-VSP L223F/C302S-mCherry pcDNA3.1 (eVSP CSmutant)
Plasmid#140893PurposeExpresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian celDepositorUseTagsmCherryExpressionMammalianMutationchanged 223L to F in Dr-VSP (zebrafish TPTE), cha…PromoterAvailable sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
FusXTBE-1397-C
Plasmid#201398PurposeExpresses C terminus half of DddAtox in mammalian cellsDepositorInsertCOX8A MTS-LacZ-G1397 DddAtox-C-UGI-ATP5B 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
FusXTBE-1397-N
Plasmid#201397PurposeExpresses N terminus half of DddAtox in mammalian cellsDepositorInsertCOX8A MTS-LacZ-G1397 DddAtox-N-UGI-ATP5B 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEM-cytoBirA-2A-mCherry-SV40pA-FKF
Plasmid#79887PurposeDonor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertHA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-DR-GC-swap-cr in pBCSK+
Plasmid#126643PurposeCloning vector LbCas12a-DR-GC-swap-crRNA for expression in mammalian cellsDepositorInsertLbCas12a Full Length Direct Repeat GC Swap crRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6 PromoterAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
ND4-FusXTBE-Left TALE
Plasmid#196496PurposeExpresses left-side TALE-DddAtox Cterm half ND4-FusXTBE in mammalian cellsDepositorInsertCOX8A MTS-ND4 left TALE-G1397 DddA-C-UGI-ATP5B 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
ND4-FusXTBE-Right TALE
Plasmid#196497PurposeExpresses Right-side TALE-DddAtox Nterm half ND4-FusXTBE in mammalian cellsDepositorInsertCOX8A MTS-ND4 right TALE-G1397 DddA-C-UGI-ATP5B 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT-flk1-cytoBirA-2A-mCherry_Ras
Plasmid#80056PurposeFlk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseTags3x HAExpressionBacterialMutationPromoterflk1Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-ubb-NLS-BirA-2a-mCherry
Plasmid#79886PurposeUbiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseTags3x HAExpressionBacterialMutationPromoterUbiquitinAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-EEA1
Plasmid#109508PurposeMuscle specific zebrafish expression of mKate2 fused to early endosome associated 1DepositorInsertEEA1 (eea1 Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT-ubb-Avi-Cerulean-RanGAP
Plasmid#79881PurposeUbiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
UseTagsAviExpressionBacterialMutationPromoterUbiquitin promoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF
Plasmid#119864PurposeDonor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertBirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTagsHA-tagged biotin ligase (BirA), Thosea asigna vir…ExpressionBacterialMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only