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Plasmid#79049PurposeExpresses human NEUROGENIN2 (hNGN2) and puromycin resistance gene under control of TetON promoter. This lentiviral vector is used to generate NGN2-iNs (induced neurons) from hiPSCs and hiPSC-NPCs.DepositorInserthNGN2-P2A-PuroR (NEUROG2 Human)
UseLentiviralTagsExpressionMutationPromoterTetOAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-GFP-wt-lamin A
Plasmid#17662DepositorInsertGFP-lamin A (LMNA Human)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-dCasRx-ALKBH5-PURO
Plasmid#175582PurposeExpresses dCasRx-ALKBH5 in mammalian cellDepositorInsertdCasRx-ALKBH5
UseCRISPR and LentiviralTagsFLAG-HAExpressionMammalianMutationRfxCas13d R239A/H244A/R858A/H863APromoterpMSCVAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Puro-CAG-mNG2(1-10)
Plasmid#206042PurposeRepair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.DepositorInsertAAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette
UseCRISPR and Synthetic Biology; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBABE FLAG-IKK2-EE puro
Plasmid#216790PurposeMammalian expression of constitutively active human IKK-2DepositorInsertIKK-2 (IKBKB Human)
UseRetroviralTagsFLAGExpressionMammalianMutationS177E and S181E, constitutively activePromoterLTRAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-puro-NLS-mCherry
Plasmid#185474PurposeMammalian Expression, Lentiviral vector expressing mCherry for two-colour competitive growth assay. Clone guide oligos into BsmBI site.DepositorTypeEmpty backboneUseLentiviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-dCasRx-METTL3-PURO
Plasmid#174218PurposeExpresses dCasRx-METTL3 in mammalian cellDepositorInsertdCasRx-METTL3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterpMSCVAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Puro PRMT5-CRISPRi
Plasmid#164637PurposelentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2DepositorInserttarget guide sequence PRMT5 inhibition (PRMT5 Human)
UseCRISPR and Lentiviral; InterferenceTagsExpressionMammalianMutationPromoterU6 and EF1AAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG FUCCI
Plasmid#136934Purposedonor plasmid for constitutive FUCCI expression in human cellsDepositorInsertClover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)
UseSynthetic BiologyTagsClover and mKO2ExpressionMammalianMutationPromoterCAGAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro FLAG SREBP2
Plasmid#32018DepositorInsertSREBP2 (Srebf2 Mouse)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKLV-U6gRNA(BbsI)-PGKpuro2ABFP
Plasmid#50946PurposeAn empty gRNA expression vectorDepositorInsertNone
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)-T2A-Puro
Plasmid#101039PurposeEnhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.DepositorTypeEmpty backboneUseCRISPRTags3xFLAG and T2A-PuroExpressionMammalianMutationPromoterCBhAvailable sinceNov. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV: Flag-Akaluc_SV40:PuroR
Plasmid#183048PurposeExpresses FLAG-tagged AkaLuc (Far-Red Firefly luciferase) in mammalian cells.DepositorInsertAkaLuc
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v2 Puro KIAA0141/DELE1_sg1
Plasmid#218524PurposesgRNA targeting human KIAA0141/DELE1DepositorInsertDELE1 (DELE1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v2 Puro KIAA0141/DELE1_sg2
Plasmid#218525PurposesgRNA targeting human KIAA0141/DELE1DepositorInsertDELE1 (DELE1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRIPZ-bioGEF-UBwt-PURO
Plasmid#208045PurposeEnables inducible expression of low-affinity Avitag-Ubwt; to perform bioE3; selection with puromycinDepositorInsertUb (UBC Human)
UseLentiviralTagsLow-affinity AvitagExpressionMutationPromotertetO/UBCAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti Lifeact-mRuby2 PuroR
Plasmid#84386PurposeLentiviral expression of mRuby2-tagged Lifeact with Puromycin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsmRuby2ExpressionMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXs-puro GFP-p62
Plasmid#38277DepositorInsertSequestosome-1 (Sqstm1 Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SFFV-NFE2L1-Puro
Plasmid#181917PurposeLentiviral plasmid that directs the expression of NFE2L1/Nrf1 in mammalian cells.DepositorInsertNFE2L1 (NFE2L1 Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationPromoterSFFVAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMK243 (Tet-OsTIR1-PURO)
Plasmid#72835PurposeExpresses OsTIR1 under the control of the Tet promoterDepositorInsertAAVS1-Tet-OsTIR1-PURO-AAVS1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only