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pGEX SYT9 C2B

Plasmid

#195708

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2B domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 253-344 of mouse SYT9

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2AB

Plasmid

#195709

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2AB domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 104-386 of mouse SYT9

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT1 C2A

Plasmid

#195710

Purpose

Bacterial expression plasmid encoding GST-tagged SYT1 C2A domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt1 (Syt1 Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 96-264 of mouse SYT1

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT1 C2B

Plasmid

#195711

Purpose

Bacterial expression plasmid encoding GST-tagged SYT1 C2B domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt1 (Syt1 Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 248-421 of mouse SYT1

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT1 C2AB

Plasmid

#195712

Purpose

Bacterial expression plasmid encoding GST-tagged SYT1 C2AB domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt1 (Syt1 Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 96-421 of mouse SYT1

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pET (SUMO)-Syt9

Plasmid

#195713

Purpose

Bacterial expression plasmid encoding SUMO-tagged full-length Syt9

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Rat)

Use

Tags

GST-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pET3a-UBE2G2

Plasmid

#204209

Purpose

Bacterial expression of UBE2G2

Depositor

Allan Weissman

Article

Das et al Mol Cell. 2009 Jun 26. 34(6):674-85.

Insert

UBE2G2 (Ube2g2 Mouse)

Use

Tags

Expression

Bacterial

Mutation

none

Promoter

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-NLS-PTEN

Plasmid

#206984

Purpose

Lentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with NLS

Depositor

Ming Chen

Article

Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

Insert

PTEN (PTEN Human)

Use

Lentiviral

Tags

NLS

Expression

Mammalian

Mutation

Promoter

tet-on promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28b(+)/ADK C77S

Plasmid

#201409

Purpose

Overexpresses Escherichia coli adenylate kinase C77S

Depositor

Gary Pielak

Article

Brom et al Biomol NMR Assign. 2023 Aug 26. doi: 10.

Insert

Escherichia coli adenylate kinase C77S (adk Escherichia coli)

Use

Tags

Expression

Bacterial

Mutation

changed cysteine 77 to serine

Promoter

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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NK809

Plasmid

#196406

Purpose

Strong expression of firefly and nanoluc luciferases codon-optimized for S. rosetta

Depositor

Nicole King

Article

Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

Insert

Sro5EFL-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin

Use

Luciferase

Tags

Expression

Mutation

N/A (synthetic)

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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NK810

Plasmid

#196407

Purpose

Nanoluc luciferase under S. rosetta SPAG6 promoter with internal actin-firefly control

Depositor

Nicole King

Article

Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

Insert

Sro5spag6-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin

Use

Luciferase

Tags

Expression

Mutation

N/A (synthetic)

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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NK811

Plasmid

#196408

Purpose

Nanoluc luciferase under S. rosetta SPAG6 promoter with mutated RFX binding site with internal actin-firefly control

Depositor

Nicole King

Article

Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

Insert

Sro5spag6_mut-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin

Use

Luciferase

Tags

Expression

Mutation

N/A (synthetic)

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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CSTI.pJL1

Plasmid

#199113

Purpose

In vitro expression of glycosyltransferase from Campylobacter jejuni for elaboration of sialic acid onto galactose in α2,3 linkage using CMP-sialic acid as substrate

Depositor

Michael Jewett

Article

Thames et al ACS Synth Biol. 2023 Apr 11. doi: 10.102

Insert

CSTI

Use

Tags

CAT linker, Strep-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpH-DmeIF4E_31-248_AD

Plasmid

#148517

Purpose

Bacterial Expression of DmeIF4E_31-248

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4E_31-248

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-Dm4E-T_9-44-Y10AL15A-GB1_AA

Plasmid

#148283

Purpose

Bacterial Expression of Dm4E-T_9-44-Y10AL15A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_9-44-Y10AL15A (4E-T Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGex-CS-DmCup_296-425-GB1_Z

Plasmid

#148168

Purpose

Bacterial Expression of DmCup_296-425

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmCup_296-425 (cup Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGex-CS-DmCup_296-425-L364AL368A-GB1_Z

Plasmid

#148169

Purpose

Bacterial Expression of DmCup_296-425-L364AL368A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmCup_296-425-L364AL368A (cup Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGex-CS-DmCup_296-425-Y327AL332A-GB1_Z

Plasmid

#148170

Purpose

Bacterial Expression of DmCup_296-425-Y327AL332A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmCup_296-425-Y327AL332A (cup Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGex-CS-DmCup_296-425-YLLLtoAAAA-GB1_Z

Plasmid

#148171

Purpose

Bacterial Expression of DmCup_296-425-YLLLtoAAAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmCup_296-425-YLLLtoAAAA (cup Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-Dm4E-T_9-21-GB1_Z

Plasmid

#148174

Purpose

Bacterial Expression of Dm4E-T_9-21

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_9-21 (4E-T Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge