Addgene

We narrowed to 6,564 results for: ski

Showing: 2321 - 2340 of 6564 results

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pAAV-FLEX-DTR-GFP

Plasmid

#124364

Purpose

conditional expression of a diphtheria toxin receptor (DTR)–GFP fusion protein

Depositor

Eiman Azim, Thomas Jessell

Article

Azim et al Nature. 2014 Apr 17;508(7496):357-63. do

Has Service

AAV2

Insert

DTR-GFP

Use

AAV

Tags

GFP

Expression

Mutation

Promoter

Chicken B-actin

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2 Flag-JunB

Plasmid

#29687

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

jun B oncogene (JUNB Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-eGFP

Plasmid

#157733

Purpose

mammalian expression of untagged eGFP under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoG-CaM

Plasmid

#193816

Purpose

CMV overexpression of ChemoG-CaM in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG-CaM

Use

Tags

Expression

Mammalian

Mutation

EGFP:E116D, A206K; HT7: L271E

Promoter

CMV

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_LAMA-F98 (for GFP)

Plasmid

#130714

Purpose

Plasmid encoding the bacterial expression of LAMA-F98 for GFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

LAMA-F98

Use

Tags

Hisx10

Expression

Bacterial

Mutation

Deleted first Met, Ala and Last Ser from the Enha…

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pB-EF1a-NLuc-IRES-Puro

Plasmid

#130936

Purpose

PiggyBac vector for constitutive NanoLuc expression in mammalian cells

Depositor

James Thomson

Article

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Insert

NanoLuc

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 I427T

Plasmid

#81035

Purpose

Mammalian expression of LOV I427T, a mutant with super short relaxation time

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV I427T

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

I427T

Promoter

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pMJ826

Plasmid

#39316

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Histidine 840 to Alanine (H840A)

Promoter

T7

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_nucNanobody IRES EGFP

Plasmid

#136619

Purpose

Plasmid encoding nuclear localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

SNAP-Tag-EnhancerNb-NLS (IRES EGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pSLIK-PUM1-rABE-Flag

Plasmid

#191385

Purpose

Doxycycline inducible expression of PUM1-rABE

Depositor

Stephen Floor

Article

Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

Insert

PUM1-rABE (PUM1 Human, Synthetic)

Use

Lentiviral

Tags

Flag

Expression

Mutation

Promoter

tet-inducible promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shSOD1

Plasmid

#102975

Purpose

Suppression of SOD1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shSOD1 (SOD1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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hTPST2

Plasmid

#11253

Depositor

Hyeryun Choe

Article

Choe et al Cell. 2003 Jul 25. 114(2):161-70.

Insert

TPST2 (TPST2 Human)

Use

Tags

C9 (GTETSQVAPA)

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOVSD

Plasmid

#81033

Purpose

Mammalian expression of the super-dark state of LOV2

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOVSD

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

C450A, L514K, G528A, L531E, N538E

Promoter

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pMJ841

Plasmid

#39318

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A) and Histidine 840 …

Promoter

T7

Available since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pBabe-PI(WT)-Src(WT)-mCherry

Plasmid

#87356

Purpose

PI(WT): Photo-inhibitable/blue light sensitive ; WT: wild type

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
Src (Src Mouse)

Use

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pDY1461_SERPINA1_CCA32_5avidity Cre

Plasmid

#193193

Purpose

Express Cre conditional upon RNA transcript

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

Cre

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLX311 Luciferase

Plasmid

#117735

Purpose

Open reading frame vector encoding Luciferase

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

firefly Luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pRK7-FLAG-TSC2

Plasmid

#8996

Depositor

John Blenis

Article

Tee et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

Insert

TSC2 (TSC2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

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pMJ825

Plasmid

#39315

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A)

Promoter

T7

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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FSW-HRP-TM

Plasmid

#82540

Purpose

Lentiviral vector for expressing HRP-TM in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

HRP fused to TM domain of PDGFR

Use

Lentiviral

Tags

HRP

Expression

Mammalian

Mutation

Promoter

Human Synapsin

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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