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Addgene
Showing: 2321 - 2340 of 20532 results
  1. pLenti-sgRNA

    Plasmid
    #71409
    Purpose
    There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. FgH1tUTG_hup53_Ex4

    Plasmid
    #85538
    Purpose
    Inducible expression of guide RNA (hup53_Ex4) with fluorescent GFP reporter
    Depositor
    Insert
    hup53 Ex4
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1t
    Availability
    Academic Institutions and Nonprofits only
  3. FgH1tUTG_hup53_Ex5

    Plasmid
    #85539
    Purpose
    Inducible expression of guide RNA (hup53_Ex5) with fluorescent GFP reporter
    Depositor
    Insert
    hup53 Ex5
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1t
    Availability
    Academic Institutions and Nonprofits only
  4. pAK_EF1a_CasRx_Hygro

    Plasmid
    #134840
    Purpose
    Lentiviral expression of CasRx T2A Hygromycin
    Depositor
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Tags
    T2A Hygromycin
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
  5. pAK_DR30_EF1a_CasRx_Hygro

    Plasmid
    #134841
    Purpose
    Lentiviral all in one expression of CasRx and guideRNA
    Depositor
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Tags
    T2A Hygromycin
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
  6. pAK_DR30_EF1a_CasRx_Puro

    Plasmid
    #134842
    Purpose
    Lentiviral all in one expression of CasRx and guideRNA
    Depositor
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Tags
    T2A Puromycin
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
  7. pLentiV2-dCas9-VP64

    Plasmid
    #141104
    Purpose
    Expression of gRNA and dCas9-VP64 from the same plasmid for CRISPRa
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  8. lenti.Cas9.BFP.sgRNA.parental

    Plasmid
    #196715
    Purpose
    Sortable WT-SpCas9 expression and guide cassette (1-vector system).
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  9. pJCKan

    Plasmid
    #185862
    Purpose
    The CRISPR/Cas9-and-gRNA-expressing plasmid with a G418-resistent selectable marker
    Depositor
    Insert
    PTDH3-Cas9(N); PSNR52-srRNA-TSUP4; KanMX4
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  10. pJCHph

    Plasmid
    #185863
    Purpose
    The CRISPR/Cas9-and-gRNA-expressing plasmid with a hygromycin B-resistent selectable marker
    Depositor
    Insert
    PTDH3-Cas9(N); PSNR52-srRNA-TSUP4; HphMX6
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  11. BPK1520_blastR

    Plasmid
    #175289
    Purpose
    Human expression plasmid for SpCas9 sgRNA with blasticidin co-selection
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV/U6
    Availability
    Academic Institutions and Nonprofits only
  12. pPB-spCas9-pNeo

    Plasmid
    #214120
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Insert
    spCas9
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  13. pPB-spCas9-pZeo

    Plasmid
    #214121
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Insert
    spCas9
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 2321 - 2340 of 20532 results