Addgene

We narrowed to 3,013 results for: actin

Showing: 2321 - 2340 of 3013 results

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pGAD424-Rabex-5

Plasmid

#197043

Purpose

Expression of GAL4 transcriptional activation domain (AD)-Rabex-5 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

Insert

Rabex-5 (RABGEF1 Bovine)

Use

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Promoter

ADH1

Available since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pPLN-hFluc-DM-HA-GFP11-KDEL

Plasmid

#177723

Purpose

DM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

hFlucDM

Use

Tags

GFP11, HA, KDEL, and Prolactin Signal Sequence

Expression

Bacterial and Mammalian

Mutation

R188Q, R261Q (Destabilized Mutant)

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET29b-AviTag-eGFP-dspB(E184Q W330Y)-6xHis

Plasmid

#175801

Purpose

Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

Insert

Dispersin B

Use

Tags

AviTag, Hexahistidine tag, and eGFP

Expression

Bacterial

Mutation

E184Q W330Y

Promoter

T7

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFendoNA2S

Plasmid

#174730

Purpose

Expresses GFP-endoNA2-S706A fusion protein for detection of polysialic acid. The endoNA2-S706A is a noncatalytic endosialidase that lacks the ability for releasing its C-terminal chaperone domain.

Depositor

Jukka Finne

Article

Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-

Insert

endoNA2-S706A

Use

Tags

Expression

Bacterial

Mutation

Changed Serine 706 to Alanine

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDK616

Plasmid

#134202

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD) mutant

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Use

Tags

Expression

Bacterial

Mutation

K223A K226A

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pIW040

Plasmid

#134127

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD)

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Use

Tags

Expression

Bacterial

Mutation

aa 132-255 R236A

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pIW039

Plasmid

#134126

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD)

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Use

Tags

Expression

Bacterial

Mutation

aa 132-255 and K203A K206A

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pIW028

Plasmid

#134125

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD)

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Use

Tags

Expression

Bacterial

Mutation

aa 132-255 and K183A K184A

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pIW070

Plasmid

#134138

Purpose

Retroviral expression of GFP-Ska1

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

GFP-human Ska1 delta MTBD CRISPR/Cas9 hardened (SKA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

residues 1-131, CRISPR resistant

Promoter

Available since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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p3 FLAG_CMV14/OCTN2 (delta14_22)

Plasmid

#112800

Purpose

Expression of OCTN2 mutant

Depositor

Katarzyna Nalęcz

Article

Czeredys et al PLoS One. 2013 Dec 13;8(12):e82105. doi:

Insert

OCTN2/SLC22A5 (Slc22a5 Rat)

Use

Tags

3 x Flag

Expression

Mammalian

Mutation

amino acids 14_22 deleted

Promoter

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 Q147A, M151A, I154A

Plasmid

#74616

Purpose

Expresses a triple mutant truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-239, Q147A, M151A, I154A (HOOK3 Human)

Use

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

Q147A, M151A, I154A

Promoter

T7

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 D102A, E108A

Plasmid

#74618

Purpose

Expresses a double mutant truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-239, D102A, E108A (HOOK3 Human)

Use

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

D102A, E108A

Promoter

T7

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 N68A, W69A, K77A

Plasmid

#74619

Purpose

Expresses a triple mutant truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-239, N68A, W69A, K77A (HOOK3 Human)

Use

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pMRX-IPU-GFP-MYH9

Plasmid

#168273

Purpose

Expresses MYH9 in mammalian cells

Depositor

Noboru Mizushima

Article

Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

Insert

MYH9 (MYH9 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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mouse E-cadherin GFP (423)

Plasmid

#67937

Purpose

Mammalian expression of E-cadherin-GFP

Depositor

Alpha Yap

Article

Truffi et al J Cell Sci. 2014 Jun 1;127(Pt 11):2420-3

Insert

E-cadherin (Cdh1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pmCherry-HP1alpha

Plasmid

#179703

Purpose

visualization of centromere heterochromatin

Depositor

Kenji Sugimoto

Article

Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

Insert

heterochromatin protein alpha (CBX5 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pBADHISTEV_PfeE

Plasmid

#129120

Purpose

pBADHISTEV carrying pfeE for overexpression of PfeE in E. coli

Depositor

James Naismith

Article

Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

Insert

Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)

Use

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

Signal peptide sequence has been removed

Promoter

PBAD

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-hIP-dCas9-BSD

Plasmid

#183231

Purpose

Lentiviral vectors with the human insulin promoter driving expression of dCas9, in addition to a U6-driven sgRNA

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Human insulin promoter

Available since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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tetO-SOX9

Plasmid

#170703

Purpose

doxycycline-inducible overexpression of SOX9

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

SOX9 (SOX9 Human)

Use

Lentiviral; Doxycycline inducible

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet-ON

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-MITF

Plasmid

#170695

Purpose

doxycycline-inducible overexpression of MITF

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

MITF (MITF Human)

Use

Lentiviral; Doxycycline inducible

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet-ON

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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