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Plasmid#133626PurposeExpression vector for mCherry fused to a tandem repeat of the E. coli MreB membrane targeting sequence (MTS) (N-terminal fusion) and a C-terminal His-tag for purification.DepositorInsert2xMreB-mCherry-His
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOV2 V416I
Plasmid#81045Purposemammalian expression of LOV V416IDepositorInsertLOV V416I
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationV416IPromoterAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cry2 mCherry GRK3 ct in pcDNA3.1
Plasmid#64213PurposemCherry fused between Cry2 and GRK3 ct to study cell migrationDepositorUseTagsmcherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
GW1-mito-mCherry-roGFP2
Plasmid#107220PurposeMammalian expression plasmid to target the mCherry-roGFP2 sensor to the mitochondrial matrix.DepositorInsertmCherry-roGFP2
UseTags4xCox8ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-pur-CAG-mCherry
Plasmid#159458PurposeAAVS1 targeting donor plasmid with mCherry expression cassette and puromycin selection geneDepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-IDR1-mCherry-Cry2
Plasmid#177125PurposeExpress IDR1 fragment of YTHDC1 with mCherry and CRY2 tag for Optodroplet AssayDepositorInsertYTHDC1 (IDR1 domain) (YTHDC1 Human)
UseTagsmCherry-CRY2ExpressionMammalianMutationIDR1 domain onlyPromoterSFFVAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB80-LAMP1-mCherry-iLID
Plasmid#174625PurposeOptogenetic coupling to lysosomes (LAMP1) via iLIDDepositorInsertLAMP1-mCherry-iLID (LAMP1 Human, Synthetic)
UseTagsmCherry-iLIDExpressionMammalianMutationmCherry: Met1DelPromoterChicken beta-actinAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
P2A-mCherry-EhT backbone
Plasmid#215549PurposeAn empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of hygromycin-NLS-tdTomatoDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tol2-ngn1-Trpa1b-2A-mCherry
Plasmid#106425PurposeFor expressing Trpa1b-2A-mCherry in zebrafish Rohon-Beard neurons.DepositorInsertsUseGatewayTags2A-mCherryExpressionMutationPromoterAvailable sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-PAmCherry-FLAG:cat
Plasmid#211832PurposePlasmid template that encodes the fluorescent protein PAmCherry1 with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out PAmcherry1-FLAG-cat for genetic tagging.DepositorInsertPAmCherry-FLAG-cat
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-LbCas12a-Plus-mCherry
Plasmid#191230PurposeTo express the LbCas12a nuclease variant "LbCas12a-Plus" that enhanced the editing activity and specificityDepositorInsertLbCas12a-Plus-P2A-mCherry
UseCRISPRTagsExpressionMutationD156R, R883K, R887APromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-NLS-BirA-2A-mCherry_Ras
Plasmid#80067PurposeBeta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterBeta-actin2Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CRY2–mPKCε-CAT-HA
Plasmid#190483PurposeExpresses a fluorescently-tagged, optogenetically activated PKC-epsilon. Used in tandem with CIBN-CAAXDepositorInsertmCherry-CRY2–mPKCε-CAT-HA (Prkce Mouse, Synthetic)
UseTagsFused to CRY2, HA, and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LINXa3-nano
Plasmid#73616PurposeLight-induced nuclear export switch fusion to nano for microscopy in mammalian cells.DepositorInsertLINXa3-nano
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPD_Psra-6::ChR2(H134R)::mCherry
Plasmid#117419PurposeFor selectively expressing ChR2(H134R) in ASH sensory neurons under the expression of a Psra-6 promoterDepositorInsertchannel rhodopsin-2 with H134R mutation
UseTagsExpressionWormMutationH134RPromotersra-6Available sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
shRNA Caspase 1 (pSicoR mCherry)
Plasmid#53575PurposeshRNA against human pro-caspase 1DepositorInsertshRNA Caspase 1 (CASP1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNA; EF1a for mCherryAvailable sinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pB80-2xFKBP-L-mCherry-CAAX
Plasmid#193713PurposeRapalog-inducible coupling to plasma membrane (CAAX) via FKBPDepositorInsert2xFKBP-L-mCherry-CAAX
UseTags2xFKBP-mCherryExpressionMammalianMutationmCherry: Met1 DeletedPromoterChicken beta-actinAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-TRE3G_3xFLAG-PGC1a4_CMV-mCherry:T2A:Hygro
Plasmid#154263PurposeDox/Tet-inducible expression of the Human PGC-1a4 splice variant in mammalian cells.DepositorInsert3xFLAG-PGC-1a4 splice variant (PPARGC1A Human)
UseTags3xFLAGExpressionMammalianMutationPromoterTRE3GAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-mHP1alpha-I163A
Plasmid#181889PurposeSoluble light-dependent effector protein (HP1alpha with I163A mutation) for optodroplet formation or recruitment to CIBN localizers,DepositorInsertPHR-mCherry-mHP1alpha-I163A (Cbx5 Mustard Weed, Mouse)
UseTagsPHR-mCherryExpressionMammalianMutationI163APromoterCMVAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-FUSN-mHP1alpha
Plasmid#181892PurposeSoluble light-dependent effector protein (HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN) ) for optodroplet formation or recruitment to CIBN localizersDepositorInsertPHR-mCherry-FUSN-mHP1alpha (Cbx5 Mustard Weed, Mouse)
UseTagsPHR-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only