-
Plasmid#214924PurposeYeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)DepositorInserttopoisomerase II
UseTags6x His-TEVExpressionYeastMutationDeleted amino acids 1178-1428PromoterGal 1/10Available sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCD-Ugi22
Plasmid#207004PurposepGGC000 Greengate entry vector containing Utricularia gibba insulator 22 (Ugi22) sequence (ID2547)DepositorInsertUtricularia gibba insulator 22
UseGolden gate compatible cloning vectorTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCD-Ugi21
Plasmid#207005PurposepGGC000 Greengate entry vector containing Utricularia gibba insulator 21 (Ugi21) sequence (ID9063)DepositorInsertUtricularia gibba insulator 21
UseGolden gate compatible cloning vectorTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT9 C2A
Plasmid#195707PurposeBacterial expression plasmid encoding GST-tagged SYT9 C2A domainDepositorInsertSyt9 (Syt5 Mouse)
UseTagsGSTExpressionBacterialMutationencodes amino acids 124-211 of mouse SYT9PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT9 C2B
Plasmid#195708PurposeBacterial expression plasmid encoding GST-tagged SYT9 C2B domainDepositorInsertSyt9 (Syt5 Mouse)
UseTagsGSTExpressionBacterialMutationencodes amino acids 253-344 of mouse SYT9PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT9 C2AB
Plasmid#195709PurposeBacterial expression plasmid encoding GST-tagged SYT9 C2AB domainDepositorInsertSyt9 (Syt5 Mouse)
UseTagsGSTExpressionBacterialMutationencodes amino acids 104-386 of mouse SYT9PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT1 C2A
Plasmid#195710PurposeBacterial expression plasmid encoding GST-tagged SYT1 C2A domainDepositorInsertSyt1 (Syt1 Rat)
UseTagsGSTExpressionBacterialMutationencodes amino acids 96-264 of mouse SYT1PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT1 C2B
Plasmid#195711PurposeBacterial expression plasmid encoding GST-tagged SYT1 C2B domainDepositorInsertSyt1 (Syt1 Rat)
UseTagsGSTExpressionBacterialMutationencodes amino acids 248-421 of mouse SYT1PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT1 C2AB
Plasmid#195712PurposeBacterial expression plasmid encoding GST-tagged SYT1 C2AB domainDepositorInsertSyt1 (Syt1 Rat)
UseTagsGSTExpressionBacterialMutationencodes amino acids 96-421 of mouse SYT1PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET (SUMO)-Syt9
Plasmid#195713PurposeBacterial expression plasmid encoding SUMO-tagged full-length Syt9DepositorInsertSyt9 (Syt5 Rat)
UseTagsGST-SUMOExpressionBacterialMutationPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET3a-UBE2G2
Plasmid#204209PurposeBacterial expression of UBE2G2DepositorInsertUBE2G2 (Ube2g2 Mouse)
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-NLS-PTEN
Plasmid#206984PurposeLentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with NLSDepositorInsertPTEN (PTEN Human)
UseLentiviralTagsNLSExpressionMammalianMutationPromotertet-on promoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28b(+)/ADK C77S
Plasmid#201409PurposeOverexpresses Escherichia coli adenylate kinase C77SDepositorInsertEscherichia coli adenylate kinase C77S (adk Escherichia coli)
UseTagsExpressionBacterialMutationchanged cysteine 77 to serinePromoterAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
NK809
Plasmid#196406PurposeStrong expression of firefly and nanoluc luciferases codon-optimized for S. rosettaDepositorInsertSro5EFL-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
UseLuciferaseTagsExpressionMutationN/A (synthetic)PromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
NK810
Plasmid#196407PurposeNanoluc luciferase under S. rosetta SPAG6 promoter with internal actin-firefly controlDepositorInsertSro5spag6-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
UseLuciferaseTagsExpressionMutationN/A (synthetic)PromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
NK811
Plasmid#196408PurposeNanoluc luciferase under S. rosetta SPAG6 promoter with mutated RFX binding site with internal actin-firefly controlDepositorInsertSro5spag6_mut-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
UseLuciferaseTagsExpressionMutationN/A (synthetic)PromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
CSTI.pJL1
Plasmid#199113PurposeIn vitro expression of glycosyltransferase from Campylobacter jejuni for elaboration of sialic acid onto galactose in α2,3 linkage using CMP-sialic acid as substrateDepositorInsertCSTI
UseTagsCAT linker, Strep-tagExpressionBacterialMutationPromoterT7Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpH-DmeIF4E_31-248_AD
Plasmid#148517PurposeBacterial Expression of DmeIF4E_31-248DepositorInsertDmeIF4E_31-248
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-Dm4E-T_9-44-Y10AL15A-GB1_AA
Plasmid#148283PurposeBacterial Expression of Dm4E-T_9-44-Y10AL15ADepositorInsertDm4E-T_9-44-Y10AL15A (4E-T Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGex-CS-DmCup_296-425-GB1_Z
Plasmid#148168PurposeBacterial Expression of DmCup_296-425DepositorInsertDmCup_296-425 (cup Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only