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Plasmid#16389DepositorInsertPKC delta isoform 2 (Prkcd Mouse)
UseTagsHAExpressionMammalianMutationDominant negative (kinase inactive): K376RPromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
KSHV ORF36 KD
Plasmid#26696DepositorInsertORF36 (ORF36 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTagsHAExpressionMammalianMutationKinase deficient mutant Lys 108 mutated to GlnPromoterAvailable sinceNov. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
PKC beta II DN
Plasmid#16385DepositorInsertPKC beta II (Prkcb Mouse)
UseTagsHAExpressionMammalianMutationDominant negative (kinase inactive): K371RPromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-PIP5K1A_IDG-K
Plasmid#214462PurposeGateway destination clone of PIP5K1A (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-PIP5K1A (PIP5K1A Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG.PKCzeta.K/W
Plasmid#10800DepositorInsertPKC zeta (Prkcz Rat)
UseTagsFLAGExpressionMammalianMutationKinase dead mutant: K281W (Lys 281 to Trp)PromoterAvailable sinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJK220
Plasmid#71123PurposeExpression of E. coli pantotheinate kinase (H6PanK, R175H mutant) in E. coliDepositorInsertpantothenate kinase
UseTagsH6ExpressionBacterialMutationArg175 is mutated to a HisPromoterT7Available sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
HMEKc-srcM
Plasmid#1107DepositorInsertc-src
UseTagsExpressionYeastMutationMET3 promoter inserted in EcoRI and BamHI of pRS3…PromoterAvailable sinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-STK17A-V5-miniTurbo_IDG-K
Plasmid#214466PurposeGateway destination clone of STK17A (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertSTK17A-V5-miniTurbo (STK17A Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJK599
Plasmid#72524PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, and associated hypothetical protein (AaPurS, AaOrfY, AaPurQ, AaPurLH6)DepositorInsertsformylglycinamidine ribonucleotide synthetase, small subunit
hypothetical protein in purSQL operon
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit
UseTagsExpressionBacterialMutationsynthetic genePromoterT7 and n/aAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK272
Plasmid#71562PurposeProduces Crepis alpina delta12 fatty acid acetylenase (vFAD2) in yeastDepositorInsertdelta12 fatty acid acetylenase
UseTagsExpressionBacterial and YeastMutationPromoterGAL1Available sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEff
Plasmid#97423PurposeTransient expression of recombinant proteins in plants. The plasmid contains gfp, which is replaced by the gene of interest.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA HA FAK Y925F
Plasmid#50509DepositorInsertprotein tyrosine kinase, mutated
UseTagsHAExpressionMammalianMutationY925FPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTol2_LSEC:Cas9green; erbb2_gRNA171
Plasmid#199338PurposepDEL135; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous erbb2 sgRNADepositorInsertsLSEC
Cas9
erbb2 sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Co-InCreN
Plasmid#51267PurposeThe N-terminal component of the Co-InCre system, expresses the N-Cre-N-gp41-1 fusion that generates functional Cre by protein trans-splicing upon reacting with Co-InCreCDepositorInsertCo-InCreN
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Co-InCreC
Plasmid#51268PurposeThe C-terminal component of the Co-InCre system, expresses the C-gp41-1-C-Cre fusion that generates functional Cre by protein trans-splicing upon reacting with Co-InCreNDepositorInsertCo-InCreC
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
zCREST3:redCas9; control_gRNA
Plasmid#199334PurposepDEL157; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous control sgRNADepositorInsertszCREST3
Cas9
control sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA HA FAK Y397F
Plasmid#50503DepositorInsertprotein tyrosine kinase, mutated
UseTagsHAExpressionMammalianMutationY397FPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pBAV150
Plasmid#79764Purposebinary DEST vector for plant expressionDepositorTypeEmpty backboneUseTetrTagsExpressionPlantMutationPromoterDEXAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
hGFAP-NLS-HA-Dre-P2A-BFP
Plasmid#51277PurposeCo-expression of the site-specific recombinase Dre and BFP under the control of the hGFAP promoterDepositorInsertNLS-HA-Dre-P2A-BFP
UseTagsExpressionMutationPromoterhGFAPAvailable sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only