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pPLN-hFluc-WT-HA-GFP11-KDEL
Plasmid#177722PurposeFirefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminusDepositorInserthFluc
UseTagsGFP11, HA, KDEL, and Prolactin Signal SequenceExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-FNIP1
Plasmid#49175PurposeMammalian expression of mouse folliculin interacting protein 1 (FNIP1) fused to GFPDepositorInsertFolliculin Interacting Protein 1 (Fnip1 Mouse)
UseTagsGFPExpressionMammalianMutationStop codon deletedPromoterCMVAvailable sinceDec. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
tetO-TBX20
Plasmid#170705Purposedoxycycline-inducible overexpression of TBX20DepositorInsertTBX20 (TBX20 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
tetO-SNAI2
Plasmid#170702Purposedoxycycline-inducible overexpression of SNAI2DepositorInsertSNAI2 (SNAI2 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH2B-CyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b
Plasmid#40571DepositorInsertAurora B FRET-sensor N-terminally fused to histone 2B
UseTagsCyPet and YPetExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLV-rtTA-zeo
Plasmid#183233PurposeLentiviral vector expressing the rtTa geneDepositorInsertrtTA-T2A-Zeo
UseLentiviralTagsExpressionMutationPromoterUBCAvailable sinceOct. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IPU-FLAG-NEK9T210A
Plasmid#168270PurposeExpresses kinase-dead NEK9 mutant in mammalian cellsDepositorInsertNEK9 (NEK9 Human)
UseTagsFLAGExpressionMammalianMutationchanged threonine 210 to alaninePromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPLN-hFluc-DM-eGFP-KDEL
Plasmid#177728PurposeDM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminusDepositorInserthFlucDM
UseTagsKDEL, Prolactin Signal Sequence, and eGFPExpressionBacterial and MammalianMutationR188Q, R261Q (Destabilized Mutant)PromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
MigR1-AEtr
Plasmid#12432DepositorUseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
Cntn4.2-AP-His
Plasmid#71942PurposeExpresses the entire Contactin 2, isoform 2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertCntn4.2 (Cntn4 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
EGFP-IC2-N106
Plasmid#51411Purposeexpression of GFP-tagged N-terminal AA 1-106 of dynein intermediate chain IC2C in mammalian cellsDepositorInsertdynein intermediate chain IC2C (Dync1i2 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIS1-wt RhoA 3'UTR
Plasmid#26089DepositorInsertwt RhoA 3'UTR (RHOA Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
Cntn4.2-Fc-His
Plasmid#72068PurposeExpresses the entire Contactin 2, isoform 2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertCntn4.2 (Cntn4 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJS278
Plasmid#134139PurposeBacterial expression of human Ska1 microtubule binding domain (MTBD)DepositorInsertHis-Precission-human Ska1 MTBD (SKA1 Human)
UseTagsExpressionBacterialMutationaa 132-255, R155APromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEG03
Plasmid#73841PurposeEncodes PhyB917 with a C-terminal histidine tag, for protein expression in Dicty.DepositorInsertPhyB(1-917) with C-term his tag (PIL2 Mustard Weed)
UseDictyostelium discoideum expressionTags6x his tagExpressionMutationPromoterAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 I136A, I139A, M142A
Plasmid#74617PurposeExpresses a triple mutant truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-239, I136A, I139A, M142A (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationD102A, E108APromoterT7Available sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
TAK4
Plasmid#227094PurposeLuciferase reporter vector with MMTV 5' Element cloned downstream of luciferase gene in pHMRΔelucDepositorInsertMMTV insert spanning from R region to 400 bp of Gag
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF-BOS-LGP2-Myc
Plasmid#215874PurposeLGP2-Myc expression vector for overexpressionDepositorInsertLGP2 (DHX58 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
tetO-TCF21
Plasmid#170706Purposedoxycycline-inducible overexpression of TCF21DepositorInsertTCF21 (TCF21 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only
