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Plasmid#195970PurposeGateway p3E 2A fluorophoreDepositorInsert2A-mCherry
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-DysF
Plasmid#109492PurposeMuscle specific zebrafish expression of mKate2 fused to dysferlinDepositorInsertDysF (dysf Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCK052 p5E exorh:mCherry
Plasmid#195953PurposeGateway p5E vector with reporterDepositorInsertexorh:mCherry
UseOtherTagsExpressionMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-21a_FnCpf1_2xNLS_protein_expression
Plasmid#124548PurposeProtein expression plasmid for 2xNLS FnCpf1 in pET-21a backboneDepositorInsertFnCpf1_2xNLS
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-GFP-U6ac-rac2-guides
Plasmid#168241Purpose"neutrophil specific GFP with ubiquitous rac2 sgRNAs"DepositorInsertrac2 sgRNAs
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,
Plasmid#110201PurposeMelanocyte-specific expression of the LexPR-2A-Cerulean fusionDepositorInsertLexPR transactivator - 2A - Cerulean
UseTagsCeruleanExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK007 pME KalTa4
Plasmid#195965PurposeGateway pME Gal4 variantDepositorInsertKalTA4 (Gal4)
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
783 pCScherryActMEK
Plasmid#31880DepositorInsertMEK1 (MAP2K1 Human)
UseExpression vectorTagsmCherryExpressionMutationSer218Glu Ser222Asp; activated MEKKPromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-DR-cr in PBCSK-
Plasmid#126641PurposeCloning vector LbCas12a DR-crRNA for expression in mammalian cellsDepositorInsertLbCas12a Full Length Direct Repeat crRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6 PromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-GFP-U6ac-cdk2-guides
Plasmid#168250Purpose"neutrophil specific GFP with ubiquitous cdk2 sgRNAs"DepositorInsertcdk2 sgRNAs
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME-nls-sfGFP
Plasmid#132973Purposefull-length of nuclear localized sfGFP with stop codon in pmE Gateway vectorDepositorInsertnuclear localized super folder GFP
UseGateway vectorTags6x myc and SV40 NLSExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2-ER-EmEGFP
Plasmid#169000PurposeExpress ER-EmEGFP in zebrafishDepositorInsertER-EGFP (encoding the signal sequence of human calreticulin mRNA and EGFP-KDEL) (CALR Human, Synthetic)
UseUnspecifiedTagsEGFPExpressionMutationPromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS-foxc1a-2a-nlstdTomato
Plasmid#132970Purposefull-length of foxc1a in frame with viral 2a peptide and nuclear localized tdTomato in pCS expression vectorDepositorInsertsUseTagsNLS and mycExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMT-MCS-cytoBirA-2A-mCherry_Ras
Plasmid#80058PurposeMCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseTags3x HAExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3E-H2B-mCerulean3-P2A-mKOFP2-CAAX
Plasmid#75177PurposeNuclear mCerulean3 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with C-terminal fusions.DepositorInsertH2B-mCerulean3-P2A-mKOFP2-CAAX
UseThree prime entry vector for multisite gateway th…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-ubb-cytoBirA-2a-mCherry
Plasmid#79885PurposeUbiquitin promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseTags3x HAExpressionBacterialMutationPromoterUbiquitinAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-EHD2b
Plasmid#109517PurposeMuscle specific zebrafish expression of mKate2 fused to EHD2bDepositorInsertEHD2b (ehd2 Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCK051 p5E exorh:mCerulean
Plasmid#195952PurposeGateway p5E vector with reporterDepositorInsertexorh:mCerulean
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-21a_2xNLS_FnCpf1_MBP_protein_expression
Plasmid#120801PurposeProtein expression plasmid for 2xNLS FnCpf1 in pET-21a backboneDepositorInsert2xNLS_FnCpf1
UseCRISPRTagsMBP and NLSExpressionBacterialMutationPromoterT7Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
FnoCas12a-DR-cr in PBCSK-
Plasmid#126642PurposeCloning vector FnoCas12a DR-crRNA for expression in mammalian cellsDepositorInsertFnoCas12a Full Length Direct Repeat crRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6 PromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only