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Plasmid#49294PurposeGolden Gate entry vector at position 8, small linker insert, final entry vector for the assemblyDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only
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pRH8
Plasmid#101014Purposedominant negative glutamate receptor subunitDepositorInsertglutamate
UseTagsExpressionMammalianMutationCHANGED THE GLUR USING PRIMERS FOUND IN ROBERT ET…PromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pEZY19
Plasmid#18668PurposepET19b converted Gateway Destination vector for expressing His tagged fusion proteins in bacteria. See note in comments section below regarding the mutated enterokinase site.DepositorTypeEmpty backboneUseGateway destination vectorTagsHisExpressionBacterialMutationPromoterT7Available sinceJuly 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
HCMV UL97 KD
Plasmid#26688DepositorInsertUL97 (UL97 Human Cytomegalovirus (HHV-5))
UseTagsHAExpressionMammalianMutationKinase Deficient Mutant Lys 355 mutated to MetPromoterAvailable sinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
EBV BGLF4 KD
Plasmid#26692DepositorInsertBGLF4
UseTagsHAExpressionMammalianMutationKinase deficient mutant: Lysine 102 mutated to I…PromoterAvailable sinceNov. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
PKC beta I DN
Plasmid#16381DepositorInsertPKC beta 1 (Prkcb Rat)
UseTagsHAExpressionMammalianMutationDominant negative (kinase inactive): K371RPromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
PPDK
Plasmid#64037PurposeExpression of Pyruvate phosphate dikinase (PPDK) in E. coliDepositorInsertpyruvate phosphate dikinase
UseTagsnoneExpressionBacterialMutationPromoterAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-NLS-HA-B3
Plasmid#51270PurposeExpression of the site-specific recombinase B3 (mouse codon-optimized)DepositorInsertNLS-HA-B3
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJK372
Plasmid#73597PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-15 fatty acid desaturase (FAD3) with C-terminal c-myc epitopeDepositorInsertsdelta-12 fatty acid acetylenase
delta-15 fatty acid desaturase
UseTagsFLAG tag and c-myc tagExpressionYeastMutationPromoterGAL1 and GAL10Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK370
Plasmid#73595PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) with C-terminal c-myc epitopeDepositorInsertsdelta-12 fatty acid acetylenase
delta-12 fatty acid desaturase (form 2)
UseTagsFLAG tag and c-myc tagExpressionYeastMutationSilent mutation in Arg299 codon.PromoterGAL1 and GAL10Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
HHV7 U69 KD
Plasmid#26694DepositorInsertU69 (U69 Human Herpes Virus 7)
UseTagsHAExpressionMammalianMutationKinase Deficient Mutant Lys 202 mutated to AsnPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
HHV6 U69 KD
Plasmid#26690DepositorInsertU69 (U69 Human Herpes Virus 6)
UseTagsHAExpressionMammalianMutationKinase Deficient Mutant: Lys 218 mutated to MetPromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
p3E NWASP-CA
Plasmid#105968Purpose3' element for Gateway cloning, NWASP C terminal domainDepositorInsertNWASP-CA
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDV
Plasmid#128860PurposeDestination vector for modules assemblyDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial, Mammalian, and Y…MutationPromoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
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FLAG.PKCzeta.T410A
Plasmid#10801DepositorInsertPKC zeta (Prkcz Rat)
UseTagsFLAGExpressionMammalianMutationActivation loop inactive: T410A (Thr 410 to Ala)PromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCiVSP-SD64TF
Plasmid#80332PurposeExpresses Ciona VSP in Xenopus OocyteDepositorInsertCiona intestinalis voltage-sensing phosphatase (vsp Ciona intestinalis)
UseXenopus oocyte expressionTagsExpressionMutationPromoterSP6Available sinceOct. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-TYR
Plasmid#32780DepositorInsertTyrosinase (TYR Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pT7-pelB
Plasmid#157740Purposeempty vector; N-terminal PelB signal sequence; potential C-terminal His6; T7 promoter; like pET-20b, but additionally avoids added residues in final protein since cloning uses BseRI restriction sitesDepositorTypeEmpty backboneUseTagsPelB signal sequence and possible His6-tag (adds …ExpressionBacterialMutationPromoterT7Available sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only