Addgene

We narrowed to 4,771 results for: codon optimized

Showing: 2281 - 2300 of 4771 results

Results per page:

Sort results by:

pMM114

Plasmid

#127211

Purpose

BeYDV replicon with constitutive Luc expression, WUS2 and IPT, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

WUS2, IPT, sgRNA, Luc

Use

Tags

Expression

Plant

Mutation

codon optimized CDSs

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
HERV-Kcon-RT for Split-PE (pJUL3120)

Plasmid

#190107

Purpose

Expresses HERV-Kcon RT (human codon optimization)

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

HERV-Kcon-RT

Use

Tags

bpNLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221_SLC22A2

Plasmid

#131964

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

Insert

SLC22A2 (SLC22A2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pZyxin-mMaple3

Plasmid

#101151

Purpose

Fluorescent protein for superresolution imaging and a focal adhesion protein for binding substrate

Depositor

Xiaowei Zhuang

Article

Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

Insert

human codon optimized mMaple3 fused to zyxin

Use

Tags

Expression

Mammalian

Mutation

Promoter

cmv

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
puc19-pCas13a

Plasmid

#118963

Purpose

Intermediate/cloning vector that can be used for sub-cloning into final destination vectors based on one's requirements.

Depositor

Magdy Mahfouz

Article

Aman et al Genome Biol. 2018 Jan 4;19(1):1. doi: 10

Insert

Cas13a

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCL.139

Plasmid

#184994

Purpose

Express Cas9-P2A-Eco1RT in human cells

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

SpCas9-P2A-Eco1RT

Use

Tags

SV40NLS

Expression

Mammalian

Mutation

Human codon optimized RT

Promoter

TetOn-3g

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRCC-N

Plasmid

#81192

Purpose

Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

Depositor

Eckhard Boles

Article

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please see depositor comments below. and WT - cod…

Promoter

ROX3 and SNP52p

Available since

Sept. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSL2620 (pTniQ_Vch)

Plasmid

#200892

Purpose

Expresses human codon-optimized V. cholerae TniQ from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchTniQ

Use

Tags

NLS

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-hisMBP-AsCpf1-EC

Plasmid

#79007

Purpose

Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)

Depositor

Jin-Soo Kim

Article

Hur et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Insert

AsCpf1

Use

Tags

6xHis-MBP-NLS and NLS-HA

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA5FRT/TO_HA-mAID-nanobody

Plasmid

#117713

Purpose

Expression of tetracycline-inducible HA-tagged lysine-less mAID fused to lysine-less vhhGFP4 (mAID-nanobody)

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

mAID-nanobody

Use

Tags

triple HA

Expression

Mammalian

Mutation

codon optimized mAID for murine expression

Promoter

CMV

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-T3-eSpCAS9-NG-pA

Plasmid

#131466

Purpose

Expresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii

Article

Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

Insert

Codon optimized eSpCas9-NG

Use

CRISPR

Tags

Flag, NLS, and polyA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRBC-sfGFP-134/150TAG

Plasmid

#174077

Purpose

Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-134/150TAG with C-terminal His6 tag, under T7 promoter

Depositor

Ryan Mehl

Article

Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

Insert

Superfolder GFP

Use

Tags

His-6

Expression

Bacterial

Mutation

D134TAG + N150TAG

Promoter

T7

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM6-C4-mCherry

Plasmid

#66530

Purpose

Expresses mCherry under the control of Mutant T7 Promoter C4

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Insert

mCherry

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon Optimized for E. coli

Promoter

Mutant T7 Promoter - C4

Available since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD287 (mKate2^SEC^3xMyc

Plasmid

#70685

Purpose

mKate2^SEC^3xMyc vector with ccdB sites for cloning homology arms

Depositor

Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

mKate2-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xMyc and C. elegans codon-optimized mKate2

Expression

Worm

Mutation

Promoter

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK365 (Tet-OsTIR1-loxP-PURO-loxP)

Plasmid

#121185

Purpose

OsTIR1 introduction

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Tet-OsTIR1-loxP-PURO-loxP

Use

CRISPR

Tags

Expression

Mutation

OsTIR1 codon optimized for human cells

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-DU422gp160

Plasmid

#100926

Purpose

Mammalian expression plasmid for Env from the DU422.1 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (DU422.1) Env

Use

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK1035

Plasmid

#8832

Depositor

David Waugh

Article

Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

Insert

TVMV protease

Use

Tags

His and MBP (cleaved)

Expression

Bacterial

Mutation

synthetic gene with codons optimized for E. coli

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
EcSTH-LbNOX

Plasmid

#211924

Purpose

This plasmid contains human codon optimized sequence of EcSTH-LbNOX which can be used to decrease NADPH/NADP ratio in mammalian cells.

Depositor

Binghui Li

Article

Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

Insert

EcSTH-LbNOX

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-B41gp160

Plasmid

#123247

Purpose

Mammalian expression plasmid for Env from the B41 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (B41) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Gi-c001

Plasmid

#173078

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

GNAI1 (GNAI1 Human)

Use

Tags

mCitrine

Expression

Bacterial

Mutation

codon optimized

Promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge