Addgene

We narrowed to 3,970 results for: KRAS

Showing: 2281 - 2300 of 3970 results

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hPGK_V5-APEX2-GFP

Plasmid

#105285

Purpose

drives expression of V5-NES-APEX2-GFP from human PGK promotor

Depositor

Eugene Yeo

Article

Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

Insert

V5-APEX2-NES

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

human PGK promoter

Available since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pGFP-HBO1-G485A pc2201

Plasmid

#167570

Purpose

The human HBO1 is fused to C-terminal GFP of EGFP-C1 (Clontech)

Depositor

Cristina Cardoso

Article

Heinz et al Epigenetics Chromatin. 2019 Mar 14;12(1)

Insert

histone acetyltransferase 1 (KAT7 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

G485A

Promoter

CMV

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-hsp70I:QFGal4-SV40pA

Plasmid

#155120

Purpose

Tol2 vector for heat-shock inducible expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

hsp70l:QFGal4 (hsp70l Budding Yeast, Zebrafish, Neurospora crassa)

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

hsp70l

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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PET-B-eSpCas9-NLS-6xHis

Plasmid

#207383

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-eSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

K848A, K1003A, R1060A, amino acids 1005-1013 repl…

Promoter

T7

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadCBEd-V106W

Plasmid

#193839

Purpose

Express TadCBEd-V106W (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-CDd V106W-SpCas9 D10A-2xUGI

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pKL123

Plasmid

#187889

Purpose

AAVS1 safe-harbor integration of dox-inducible NFIA-SOX9

Depositor

Martin Kampmann

Article

Leng et al Nat Neurosci. 2022 Nov;25(11):1528-1542.

Insert

NFIA-SOX9

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLIX403_UBAP2L_mCherry

Plasmid

#105286

Purpose

puro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion protein

Depositor

Eugene Yeo

Article

Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

Insert

UBAP2L (UBAP2L Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Available since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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3XFLAG_GAPDH in pCW57_tTA_Blast

Plasmid

#154211

Purpose

Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of GAPDH

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

GAPDH (GAPDH Human)

Use

Lentiviral

Tags

3X FLAG

Expression

Mammalian

Mutation

Promoter

TRE repeats (dox off)

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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peGFP C2-IST1

Plasmid

#218582

Purpose

Expresses GFP-tagged human IST1 in mammalian cells

Depositor

Hemmo Meyer

Article

Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

Insert

IST1 (IST1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-MAP1B

Plasmid

#44396

Depositor

Phillip Gordon-Weeks

Article

Scales et al J Cell Sci. 2009 Jul 15;122(Pt 14):2424-

Insert

MAP1B (Map1b Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 21, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX4T-hLIG3

Plasmid

#81055

Purpose

bacterial expression of human LIG3

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

DNA ligase 3 (LIG3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV_OHu19986C_myc tag

Plasmid

#183174

Purpose

used to make AAV vectors for hsTRPA1 tagged with myc

Depositor

Sreekanth Chalasani

Article

Duque et al Nat Commun. 2022 Feb 9;13(1):600. doi: 1

Insert

hsTRPA1 (TRPA1 Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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p2T-cmv-SpCas9-TadCBEd-V106W-BlastR

Plasmid

#193849

Purpose

Express TadCBEd-V106W in mammalian cells with BlastR selection, Tol2 integration

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadCBEd-V106W

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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7TFP CDH1 reporter

Plasmid

#91704

Purpose

lentiviral luciferase reporter containing CDH1 promoter

Depositor

Bob Weinberg

Article

Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

Insert

CDH1 promoter (CDH1 Human)

Use

Lentiviral and Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

CDH1

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pDY1462_SERPINA1_CCA32_Caspase

Plasmid

#193194

Purpose

Express Caspase conditional upon RNA transcript

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

iCaspase9 (CASP9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pRDB_053

Plasmid

#216083

Purpose

for transgenic flies; gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31, destination vector

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31

Use

Destination

Tags

Expression

Insect

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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DRH296: FCK-Optopatch2

Plasmid

#51694

Purpose

Coexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

Optopatch2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

June 23, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-J chain

Plasmid

#145146

Purpose

Mammalian expression plasmid for J chain of polymeric antibody classes

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

immunoglobulin J chain (JCHAIN Human)

Use

Tags

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-acta1:QFGal4-SV40pA

Plasmid

#155121

Purpose

Tol2 vector for skeletal-muscle expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

acta1:QFGal4

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

acta1

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-tagRFP-BleoR

Plasmid

#192508

Purpose

Expression of PE2 in mammalian cells with co-expression of tagRFP and Zeocin resistance gene.

Depositor

Gerald Schwank

Article

Mathis et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

PE2-P2A-tagRFP-P2A-BleoR

Use

Tags

BleoR, SV40 bpNLS, and tagRFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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