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Plasmid#119967PurposeBacterial expression for structure determinationDepositorInsertLOC149281 (METTL11B Human)
UseTags6xHIS, thrombin,SUMO and TEV cleavageExpressionBacterialMutationcontains amino acids 58-278PromoterT7Available sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOEM1_pCMV:axFgf8-pPH:vsvged
Plasmid#111159PurposeBaculovirus rescue vector, VSVGED pseudotype, hShhDepositorInsertaxFgf8
UseBaculoviral rescue shuttle vectorTagsExpressionInsect and MammalianMutationwtPromoterCMVAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDS2-aurZ
Plasmid#70151PurposeFusarium graminearum AurZ encoded on CEN/ARS yeast shuttle vector with DS2 selection markerDepositorInsertaurZ
UseTagsExpressionYeastMutationPromoterTefAvailable sinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDS3-aurJ
Plasmid#70152PurposeFusarium graminearum AurJ encoded on CEN/ARS yeast shuttle vector with DS3 selection markerDepositorInsertaurJ
UseTagsExpressionYeastMutationPromoterGPD1Available sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMGB
Plasmid#139932PurposeM. magneticum/E. coli shuttle vector (partial sequence of pMGT and p15A)DepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterPJ23101Available sinceAvailabilityAcademic Institutions and Nonprofits only -
hSERT-N1-pEYFP
Plasmid#70105Purposemammalian expression of hSERT with C-terminal YFP fusionDepositorInsertSERT (SLC6A4 Human)
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFW1000
Plasmid#224210PurposeE. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), R6K (E. coli replication origin)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFW2000
Plasmid#224211PurposeE. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual-M2
Plasmid#135584PurposeHigh eukaryotic dual protein expression shuttle vectorDepositorTypeEmpty backboneUseTransfer vectorTagsExpressionInsectMutationPromoterpolyhedrin of AcMNPVAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX-TRV2
Plasmid#180516PurposeAgrobacterium tumefaciens-based expression of TRV2 including a PEBV heterologous promoter and apolylinkerDepositorInsertTobacco rattle virus RNA2 with a PEBV heterologous promoter and a polylinker
UseTagsExpressionPlantMutationPromoter35S CaMVAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEMPTY::sgRNA2
Plasmid#165459PurposeEscherichia coli – Staphylococcus aureus shuttle vector for plasmid curing in Gram-positive bacteriaDepositorInsertsgRNA2
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterSP01Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ1-promoterless-LacZ
Plasmid#191617PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), promoterless-lacZDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
p201N Cas9
Plasmid#59175PurposeCas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA ShuttleDepositorInsertsCas9
nptII
UseCRISPRTagsExpressionMutationPromoter2x35S and StUbi-3PAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOCC5
Plasmid#118863Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C proteaseDepositorInsertNcoI-HIS6-3C-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBlu/gRNA
Plasmid#59188PurposeServes as a shuttle vector for target oligo before insertion into Cas9 destination vectorDepositorInsertGuide RNA Cassette
UseCRISPRTagsExpressionPlantMutationPromoterU6 (Arabidopis)Available sinceFeb. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOCC7
Plasmid#118876Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
p201H Cas9
Plasmid#59176PurposeCas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA ShuttleDepositorInsertsCas9
hph
UseCRISPRTagsExpressionMutationPromoter2x35S and StUbi-3PAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJYS1Ptac
Plasmid#85545PurposeConstitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoterDepositorInsertsCpf1
recT
UseCRISPR; Shuttle vector corynebacterium glutamicum…TagsExpressionBacterialMutationPromotertac and unknownAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFW5000
Plasmid#224214PurposeE. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin), tetQDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAP114
Plasmid#120799PurposeE. coli-C. difficile shuttle vector for xylose-inducible expression in C. difficile; Pxyl driving expression of red fluorescent protein (mCherryOpt)DepositorInsertPxyl::mCherryOpt
UseTagsExpressionBacterialMutation1.4 kb xylR-Pxyl DNA fragment from C. difficile R…PromoterPxylAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only