Addgene

We narrowed to 15,751 results for: puromycin

Showing: 2261 - 2280 of 15751 results

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pJA317

Plasmid

#74488

Purpose

eF1alpha::puroR::mCherry::T2A::eGFP::d1ODC::wPRE has d1ODC degron

Depositor

Andrew Fire

Article

Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

Insert

d1ODC

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pRetroSuper Smad4

Plasmid

#15727

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

Smad4 shRNA (SMAD4 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
p663-UBC-miniTurbo-V5-NEK9_IDG-K

Plasmid

#189873

Purpose

Gateway destination clone of NEK9 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

NEK9 (NEK9 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pRP(Exp)-CMV> :dTomato:IRES:Puro

Plasmid

#110832

Purpose

Expresses dTomato (dT) red fluorescent reporter.

Depositor

Ande Satyanarayana

Article

Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:

Insert

dTomato Tag

Use

Non-viral gene expression vector

Tags

IRES

Expression

Mutation

Promoter

CMV

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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Retrohair

Plasmid

#8450

Depositor

Bob Weinberg

Article

Stewart et al RNA 2003 Apr;9(4):493-501.

Type

Empty backbone

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
pSuperRetro-EFP siRNA2

Plasmid

#12450

Depositor

Dong-Er Zhang

Article

Zou et al J Biol Chem. 2006 Feb 17. 281(7):3989-94

Insert

siRNA against EFP (TRIM25 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pGBW-m4258306

Plasmid

#154795

Purpose

Mammalian expression plasmid for SARS-CoV-2 E (envelope)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;V5

Expression

Mammalian

Mutation

H. sapiens recode 1

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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SNX9

Plasmid

#171943

Purpose

Lentiviral plasmid to express mouse SNX9 in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

SNX9

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pERB219

Plasmid

#61501

Purpose

Const. Halotag-GFP-PACT + mCherry-DHFR

Depositor

Michael Lampson, David Chenoweth

Article

Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

Inserts

HaloTag-GFP-PACT
mCherry-DHFR

Use

Tags

HaloTag and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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lentiGuide-Puro.3xBsmBI

Plasmid

#196709

Purpose

Backbone for cloning guide libraries with untranscribed barcodes and a tracrRNA of choice

Depositor

Bernhard Schmierer

Article

Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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p667-UBC-NUAK2-V5-miniTurbo_IDG-K

Plasmid

#189910

Purpose

Gateway destination clone of NUAK2 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

NUAK2 (NUAK2 Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pYJ21-lenti-EF1a-ASAP2f-puro

Plasmid

#131065

Purpose

Forced expression of ASAP2f

Depositor

Xiaojun Lian

Article

Jiang et al Stem Cell Res Ther. 2021 May 29;12(1):30

Insert

ASAP2f

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pBabePuro-hSNAIL.ER.NoTag

Plasmid

#19292

Depositor

Eric Fearon

Article

Hajra et al Cancer Res. 2002 Mar 15. 62(6):1613-8.

Insert

SNAIL (SNAI1 Human)

Use

Retroviral

Tags

mouse ERalpha ligand-binding domain

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pLJM1-EGFP-BarcodeV2

Plasmid

#78632

Purpose

GESTALT V2 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT V2 insert

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pGBW-m4252845

Plasmid

#153787

Purpose

Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;6xHis-003

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDest-312 (attR4-attR3)

Plasmid

#161897

Purpose

Multisite Gateway (attR4-attR3) vector for mammalian protein expression

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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LV UAS-DsRed-Express2-PGK-Puro

Plasmid

#190801

Purpose

LV construct encoding UAS-DsRed-Express2 reporter gene.

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

UAS-T2A-DsRed-Express2

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pJA318

Plasmid

#74489

Purpose

eF1alpha::puroR::mCherry::T2A::eGFP::d4ODC::wPRE has d4ODC degron

Depositor

Andrew Fire

Article

Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

Insert

d4ODC

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pBABE-FLAG-HA-hG9a

Plasmid

#33022

Depositor

Martin Walsh

Article

Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

Insert

hG9a

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
pMSCVpuro-mCerulean3

Plasmid

#96936

Purpose

Retroviral plasmid containing mCerulean3 for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Monomeric Cerulean 3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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