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Showing: 2261 - 2280 of 12947 results

  1. 3xFLAG-dCas9/pMSCVpuro

    Plasmid
    #134983
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    134983-plasmid-map-sequence-id-430623

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  3. pBabePuro-FLAG-KrascomQ61R

    Plasmid
    #206863
    Purpose
    To stably express mouse Kras encoded by common mouse codons (to increase expression) and with a Q61R mutation with an N-terminal FLAG tag in cells resistant to Neo.
    Depositor
    Christopher Counter
    Article
    Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755
    Insert
    Kras (Kras Mouse)
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Codons 1-3 altered to most optimum based on mouse…
    Promoter
    Available since
    April 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    206863-plasmid-map-sequence-id-430070

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  4. pBabePuro-FLAG-KrascomG12D

    Plasmid
    #206862
    Purpose
    To stably express mouse Kras encoded by common mouse codons (to increase expression) and with a G12D mutation with an N-terminal FLAG tag in cells resistant to Neo..
    Depositor
    Christopher Counter
    Article
    Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755
    Insert
    Kras (Kras Mouse)
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Codons in exons 1-3 altered to most optimum based…
    Promoter
    Available since
    April 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    206862-plasmid-map-sequence-id-430069

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  5. pBabePuro-FLAG-KrasnatG12D

    Plasmid
    #206865
    Purpose
    To stably express mouse Kras encoded by native mouse codons and with a G12D mutation with an N-terminal FLAG tag in cells resistant to Neo.
    Depositor
    Christopher Counter
    Article
    Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755
    Insert
    Kras (Kras Mouse)
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    changed Glycine 12 to Aspartic Acid
    Promoter
    Available since
    April 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    206865-plasmid-map-sequence-id-430068

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  14. eSpCas9(1.1)_NoFlag_ATP1A1_G3_SLBP_G1_Dual_sgRNA

    Plasmid
    #191528
    Purpose
    Vector for tandem expression of SLBP 3'UTR G1 sgRNA in combination with ATP1A1 G3 sgRNA from two independent U6 promoters to facilitate SLBP endogenous tagging by marker free coselection using ouabain
    Depositor
    Yannick Doyon
    Insert
    SLBP 3'UTR G1 sgRNA + ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191528-plasmid-map-sequence-id-378116

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  16. pLJM1 flag-cFLIP

    Plasmid
    #211529
    Purpose
    Expresses flag-tagged cFLIP (CFLAR)
    Depositor
    Richard Possemato
    Insert
    cFLIP (CFLAR Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    211529-plasmid-map-sequence-id-421060

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  17. pFastBac1-C17orf53-FLAG

    Plasmid
    #211447
    Purpose
    generation of Bacmid for insect cell expression of C17orf53 C-terminally fused to FLAG-Tag
    Depositor
    Helmut Pospiech
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    FLAG-Tag
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    211447-plasmid-map-sequence-id-419421

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  18. pcDNA3-hFCER1G-VenusFlag

    Plasmid
    #211899
    Purpose
    Expressess human FCER1G in mammalian cells
    Depositor
    Haian Fu
    Insert
    FCER1G (FCER1G Human)
    Use
    Tags
    VenusFlag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    211899-plasmid-map-sequence-id-419135

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  20. pET28-Flag-RBM24-1~93

    Plasmid
    #208633
    Purpose
    for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa1~93
    Depositor
    Quanfu Ma
    Insert
    human RBM24 (ENST00000216146) aa 1~93 (RBM24 Human)
    Use
    Tags
    6xHis and Flag
    Expression
    Bacterial
    Mutation
    only aa 1~93, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208633-plasmid-map-sequence-id-415921

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Showing: 2261 - 2280 of 12947 results