Addgene

We narrowed to 4,627 results for: codon optimized

Showing: 2261 - 2280 of 4627 results

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pCS2+_Flag-myc-NES-Tir1

Plasmid

#117717

Purpose

mRNA synthesis of Flag-myc-NES-TIR1 for injection

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

TRANSPORT INHIBITOR RESPONSE 1

Use

Tags

Flag-Myc-NES

Expression

Mammalian

Mutation

codon optimized TIR1 for murine expression

Promoter

SP6

Availability

Academic Institutions and Nonprofits only

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pCS2+_HA-mAID-nanobody (Kless)

Plasmid

#117718

Purpose

mRNA synthesis of lysine-less HA-mAID-nanobdy for injection

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

mAID-nanobody

Use

Tags

3xHA

Expression

Mammalian

Mutation

codon-optimized and lysine-less mAID for murine e…

Promoter

SP6

Availability

Academic Institutions and Nonprofits only

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pFS_0453_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)_T7term_ARRAY2_FaqI

Plasmid

#117006

Purpose

Expresses E. coli codon-optimized FsRT-Cas1-Cas2 under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-eKR2

Plasmid

#115337

Purpose

Expresses enhanced sodium pump for light-driven extrusion of sodium; activation with green light; codon-optimized for human/mouse

Depositor

Peter Hegemann

Article

Grimm et al Sci Rep. 2018 Jun 18;8(1):9316. doi: 10.

Insert

eKR2

Use

Tags

EYFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pscALPS- SIV-MAC251 vpx

Plasmid

#115810

Purpose

Encodes codon optimized 3xFLAG-VpxMAC251 and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMAC251 Vpx

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLAG-SIV-MAC251-vpx

Plasmid

#115842

Purpose

Encodes codon optimized FLAG tagged SIVMAC251-Vpx

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMAC251-Vpx

Use

Tags

3x-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLAG-SIV-RCM-vpx

Plasmid

#115844

Purpose

Encodes codon optimized FLAG tagged SIVRCM-Vpx

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVRCM-Vpx

Use

Tags

3x-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pMM113

Plasmid

#127210

Purpose

BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

WUS2, AtSTM, sgRNA, Luc

Use

Tags

Expression

Plant

Mutation

codon optimized CDSs

Promoter

Availability

Academic Institutions and Nonprofits only

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pMM114

Plasmid

#127211

Purpose

BeYDV replicon with constitutive Luc expression, WUS2 and IPT, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

WUS2, IPT, sgRNA, Luc

Use

Tags

Expression

Plant

Mutation

codon optimized CDSs

Promoter

Availability

Academic Institutions and Nonprofits only

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pRN227

Plasmid

#127222

Purpose

BeYDV replicon with 35S::Cas9, WUS2 and AtSTM, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Cas9, WUS2, AtSTM, sgRNA

Use

Tags

Expression

Plant

Mutation

codon optimized CDSs

Promoter

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-BirA(R118G)-NES-YFP-STOP-L2

Plasmid

#127357

Purpose

gateway entry vector for expressing cytosolic BirA* (R118G)-YFP under a promoter of choice

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

BirA* (R118G mutant)

Use

Gateway entry vector

Tags

GS linker, NES, V5, and YFP

Expression

Mutation

R118G

Promoter

no promoter

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-BirA(R118G)-YFP-NLS-STOP-L2

Plasmid

#127360

Purpose

gateway entry vector for expressing nuclear BirA* (R118G)-YFP under a promoter of choice

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

BirA* (R118G mutant)

Use

Gateway entry vector

Tags

GS linker, NLS, V5, and YFP

Expression

Mutation

R118G

Promoter

no promoter

Availability

Academic Institutions and Nonprofits only

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pCEP4-BaLgp160

Plasmid

#100919

Purpose

Mammalian expression plasmid for Env from the BaL HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Use

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCEP4-DU422gp160

Plasmid

#100926

Purpose

Mammalian expression plasmid for Env from the DU422.1 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (DU422.1) Env

Use

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pZyxin-mMaple2

Plasmid

#101150

Purpose

Fluorescent protein for superresolution imaging and a focal adhesion protein for binding substrate

Depositor

Xiaowei Zhuang

Article

Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

Insert

human codon optimized mMaple2 fused to zyxin

Use

Tags

Expression

Mammalian

Mutation

Promoter

cmv

Availability

Academic Institutions and Nonprofits only

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pZyxin-mMaple3

Plasmid

#101151

Purpose

Fluorescent protein for superresolution imaging and a focal adhesion protein for binding substrate

Depositor

Xiaowei Zhuang

Article

Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

Insert

human codon optimized mMaple3 fused to zyxin

Use

Tags

Expression

Mammalian

Mutation

Promoter

cmv

Availability

Academic Institutions and Nonprofits only

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pALPS_3'LTR GFP@gag start SFFV tat-P2A-rev

Plasmid

#101340

Purpose

LTR drives GFP and internal SFFV promoter drives codon optimized tat and rev.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

GFP, tat, rev

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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His-NavMs

Plasmid

#100004

Purpose

Electrophysiology in mammalian cells: Mammalian expression of voltage gated sodium channel and GFP

Depositor

Paul DeCaen

Article

Sula et al Nat Commun. 2017 Feb 16;8:14205. doi: 10

Insert

His-NavMs, Voltage gated sodium channel

Use

Tags

Hexa-His tag followed by a thrombin cleavage site

Expression

Mammalian

Mutation

C-terminus from a codon-optimized synthetic gene,…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP

Plasmid

#104394

Purpose

Yeast MCP-GFP expression

Depositor

Evelina Tutucci, Robert Singer

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

MCP-NLS-2xyeGFP

Use

Tags

Expression

Bacterial and Yeast

Mutation

MCP fused to NLS(SV40) and 2x yeast codon optimiz…

Promoter

CYC1

Availability

Academic Institutions and Nonprofits only

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pSBY3_ligD-HygR

Plasmid

#104625

Purpose

CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD and hygromysin resistance gene.

Depositor

Sheng Yang

Article

Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

Insert

FnCpf1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

FnCpf1 codon-optimized C.glutamicum

Promoter

hsp60

Availability

Academic Institutions and Nonprofits only

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