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Plasmid#168464PurposeFor the insertion pf NLS-mEos3.2-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mEos3.2-NLS
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD440
Plasmid#168466PurposeFor the insertion pf NLS-mCherry-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mCherry-H-NSdbd-NLS
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD441
Plasmid#168467PurposeFor the insertion pf NLS-mEGFP-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mEGFP-H-NSdbd-NLS
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD442
Plasmid#168468PurposeFor the insertion of NLS-eYFP-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-eYFP-H-NSdbd-NLS
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD444
Plasmid#168470PurposeFor the insertion of NLS-mNeonGreen-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mNeonGreen-H-NSdbd-NLS
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3HA-AMOT p130∆PPxY2
Plasmid#166712Purposeexpresses AMOT with inactivating mutation in second PPxY motif in mammalian cellsDepositorInsertAngiomotin p130 P262A P263A (AMOT Human)
UseTags2xHAExpressionMammalianMutationchanged proline 262 to alanine, proline 263 to al…PromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3HA-AMOT p130∆PPxY3
Plasmid#166713Purposeexpresses AMOT with inactivating mutation in third PPxY motif in mammalian cellsDepositorInsertAngiomotin p130 P3072A P308A (AMOT Human)
UseTags2xHAExpressionMammalianMutationchanged proline 307 to alanine, proline 308 to al…PromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
SHC003BSD-EXOC7
Plasmid#133298PurposeExpresses EXOC7 in mammalian cellsDepositorInsertExocyst complex component 7 (EXOC7 Human)
UseLentiviralTags3XFLAGExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO myc Separase delta 55/Non-Cleavable
Plasmid#59827PurposeAllows the integration of myc Separase delta 55/Non-Cleavable in the genome and Tet-inducible expression.DepositorInsertSeparase (ESPL1 Human)
UseTagsMycExpressionMammalianMutationdelta 55/Non-Cleavable (E1483R, R1486E, E1503R, R…Promoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-a I18A
Plasmid#40043DepositorInserthSlp4-a I18A (SYTL4 Human)
UseTagsEGFPExpressionMammalianMutationIsoleucine 18 to AlaninePromoterCMV promoterAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDAC439
Plasmid#195237PurposeMammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown)DepositorInsertFLAG-NLS-Csm5-2A-FLAG-NLS-Csm4-2A-FLAG-NLS-Csm3-2A-FLAG-NLS-Csm2; RFP-2A-FLAG-NLS-Cas6-2A-FLAG-NLS-Csm1; crRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
HRE-UnaG
Plasmid#201710PurposeHypoxia responsive promoter driving UnaG fluorescent proteinsDepositorInsertUnaG
UseTagsExpressionMammalianMutationPromoterHRE-mCMVAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
AbVec1.1-IGKC
Plasmid#80796PurposeExpression of immunoglobulin light chains in mammalian cells, human kappa constant regionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-IGHG1
Plasmid#80795PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotypeDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pME-nlsGFP-P2A
Plasmid#80808Purposenuclear localization signal fused to N-terminus of GFP (nlsGFP) without stop codon, followed by P2ADepositorInsertminimal cytomegalovirus immediate early enhancer/promoter
UseTagsExpressionMutationPromoterAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
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PB-GFP-Gal8
Plasmid#127191PurposePiggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.DepositorInsertGFP-Gal8 (LGALS8 Human)
UseTagsGal8 is fused to the c-terminus of GFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-beta-HA-BRCA2
Plasmid#133553PurposeMammalian expression vector with CMV promoter driving beta globin intron, HA tag, BRCA2 ORFDepositorInsertBRCA2 (BRCA2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only