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Plasmid#177802PurposeMammalian expression of green fluorescent potassium indicator KRaION2DepositorInsertKRaION2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_nLightR
Plasmid#217657PurposeExpresses norepinephrine indicator nLightR under mammalian promoterDepositorInsertnLightR
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Zeo-CnA-H151Q
Plasmid#191967PurposeExpression of dominant negative CnA H151Q mutant protein (catalytically inactive).DepositorInsertCalcineurin subunit alpha H151Q (PPP3CA Human)
UseTagsExpressionMammalianMutationH151QPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP GFP-MOSPD2 WT
Plasmid#186467PurposeExpression of human MOSPD2 fused to EGFP in mammalian cellsDepositorInsertMOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Mu-switch-R-loop
Plasmid#134899PurposeGeneration and purification of R-loops for in vitro biochemistryDepositorInsertmouse IgH switch repeat region
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFEndoNA
Plasmid#174724PurposeExpresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.DepositorInsertGFP-endoNA fusion protein
UseTagsHistagExpressionBacterialMutationPromoterT5Available sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETcLIC-GFP
Plasmid#183514PurposeThe plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coliDepositorTypeEmpty backboneUseTags10x His-tag and folding reporter GFPExpressionBacterialMutationPromoterT7 lacAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
hDAGLb-V5
Plasmid#87677PurposeExpresses human DAGLb protein with intracellular V5 tag located on the C-terminalDepositorInsertHuman Diacylglycerol Lipase Beta (DAGLB Human)
UseTagsV5 tagExpressionMammalianMutationPromoterT7Available sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
IMS-HyPer2
Plasmid#60248PurposeGenetically-encoded fluorescent hydrogen peroxide indicator HyPer2 targeted to mitochondrial intermembrane spase.DepositorInsertIMS-HyPer2
UseTagsMitochondrial intermembrane space targeting signa…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pMT-Hrp48-ADARcd-E488Q-V5
Plasmid#139685PurposeExpresses the hyperactive form of the catalytic domain of the ADAR protein fused to HRP-48 for use in "TRIBE" assaysDepositorInsertHrp48, ADAR (Adar Fly)
UseTagsV5ExpressionBacterial and InsectMutationPromoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
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Cas9-GFP_sg_mAMPKa1
Plasmid#79004PurposeExpresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.DepositorInsertPrkaa1 (Prkaa1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-JEDI-1P-WPRE
Plasmid#202613PurposeGenetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for neuron-specific expression using the promoter hSynDepositorInsertJEDI-1P
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-JEDI-1P-WPRE
Plasmid#202616PurposeGenetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for astrocytes specific expression using the promoter GFAPDepositorInsertJEDI-1P
UseAAVTagsExpressionMammalianMutationPromoterGFAPAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Yellow Cameleon-Nano140/pcDNA3
Plasmid#51966Purposemammalian expression of Yellow Cameleon-Nano140, an ultrasensitive Ca2+ indicator with Kd = 140 nMDepositorInsertYellow Cameleon-Nano140
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-KRaION2
Plasmid#177800PurposeBacterial expression of green fluorescent potassium indicator KRaION2DepositorInsertKRaION2
UseTagsExpressionBacterialMutationPromoteraraBAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-OTUB1 C91S-pcDNA3.1
Plasmid#118210PurposeExpresses FLAG-OTUB1 C91S catalytic inactive mutant in mammalian cellsDepositorInsertOTUB1 (OTUB1 Human)
UseTags3x FLAGExpressionMammalianMutationchanged cysteine 91 to serinePromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDR-GFPuniv
Plasmid#46085PurposeIn vivo recombination assay fluorescent reporterDepositorInsertDR-GFPuniv reporter
UseRecombination reporter plasmidTagsExpressionMammalianMutationE5G and K163R mutations in EGFP relative to wild-…PromoterAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-R-GECO1
Plasmid#32465DepositorArticleInsertR-GECO1.0
UseLab constructedTags6x His and Modified TorA MNNNDLFQASRRRFLAQLG[G to…ExpressionBacterialMutationSubstitutions relative to the mApple-derived anal…PromoterAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only