Addgene

We narrowed to 6,556 results for: KRAS

Showing: 2261 - 2280 of 6556 results

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pAT9784-xABE

Plasmid

#163005

Purpose

plasmid expressing xABE

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

xABE

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Mutation

Promoter

EF1-a

Available since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pME-QUASM1:GFPNLS-SV40pA

Plasmid

#155116

Purpose

Middle entry vector containing QUASM1 element upstream of GFP-NLS.

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1:GFPNLS

Use

Gateway middle entry vector

Tags

Expression

Mutation

Promoter

QUASM1-E1b

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_LynNanobody IRES EGFP

Plasmid

#136624

Purpose

Plasmid encoding outer inner membrane localization of the Enhancer nanobody GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

Lyn11-SNAP-Tag-EnhancerNb (IRES EGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAM-PAT-ProTTG1 GW

Plasmid

#79753

Purpose

binary DEST vector for plant expression

Depositor

Nico Dissmeyer, Martin Hulskamp

Article

Bouyer et al PLoS Biol. 2008 Jun 10;6(6):e141. doi: 1

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

ProTTG1

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221-CMV.Pro

Plasmid

#79486

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5), CMV promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

CMV Promoter

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

CMV Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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miR373 in pcDNA3.1

Plasmid

#78127

Purpose

Mammalian expression of miR373

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

miR373 (MIR373 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pGMCKq20-T38S38-0X

Plasmid

#49522

Purpose

Kanamycin resistant; contains qtag20 and constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 site

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

constitutive TetR38

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptb38

Available since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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p7013 MSCV-IP N-HAonly 45E6nonsplice

Plasmid

#44146

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV45E6

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Internal splice site mutated.

Promoter

MSCV LTR

Available since

April 30, 2013

Availability

Academic Institutions and Nonprofits only
4LG6

Plasmid

#60093

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

ANKRA:JMC023-D07:C211743 (ANKRA2 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

See comments

Promoter

T7

Available since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pDF0159 pCMV - huDisCas7-11 mammalian expression

Plasmid

#172507

Purpose

Encodes human codon optimized DisCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 mammalian expression

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDF0234 pCMV - huDisCas7-11 dead mutant

Plasmid

#172509

Purpose

Encodes human codon optimized catalytically dead DisCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 dead mutant

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBS1C

Plasmid

#55168

Purpose

Empty vector, integration at amyE, ampr, cmr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Cdc42(Q61L)

Plasmid

#12600

Depositor

Klaus Hahn

Article

Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

Insert

EGFP-Cdc42 (Q61L) (CDC42 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Q61L

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgRNA

Plasmid

#124845

Purpose

Vector for FLP-dependent expression of SaCas9 with gRNA

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

SauCas9 gRNA scaffold

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGAS-HA

Plasmid

#130910

Purpose

Expresses human cGAS-HA; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

cGAS

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP6450

Plasmid

#128731

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Wei et al Cell Host Microbe. 2015 Jun 10;17(6):752

Insert

HopAD1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-SAMTOR

Plasmid

#100508

Purpose

Expresses FLAG tagged SAMTOR in mammalian cells

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

SAMTOR (BMT2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-EGFP-KASH

Plasmid

#154373

Purpose

Vector for Cre-dependent expression of EGFP tagged KASH protein domain

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

EGFP-KASH

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pDAS12489_PEAR-GFP_2in1_2.0

Plasmid

#177186

Purpose

PEAR-GFP3 plasmid with a pegRNA that targets its own plasmid

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Inserts

EGFP split with an intron between amino acids 95-96
pegRNA targeting its own plasmid

Use

CRISPR

Tags

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV and U6

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-CMV-Rheb(S16H)-IRES-eGFP-W

Plasmid

#32520

Depositor

Mustafa Sahin

Article

Nie et al Nat Neurosci. 2010 Feb;13(2):163-72. Epu

Insert

Constitutively active Rheb (S16H) (Rheb Rat)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

S16H to make constitutively active

Promoter

CMV

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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