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Showing: 2241 - 2260 of 5233 results

  2. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2b_(CJT93)

    Plasmid
    #226992
    Purpose
    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2b must be used with gRNA 1a or 1b
    Depositor
    Benjamin Kleinstiver
    Article
    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.
    Insert
    SpCas9 gRNA 2b to create OPA1-del_exon5
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    226992-plasmid-map-sequence-id-451253

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  3. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1b_(CJT92)

    Plasmid
    #226990
    Purpose
    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1b must be used with gRNA 2a or 2b
    Depositor
    Benjamin Kleinstiver
    Article
    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.
    Insert
    SpCas9 gRNA 1b to create OPA1-del_exon5
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    226990-plasmid-map-sequence-id-451250

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  12. pCAGGS-iRFP713(C15S/V256C)-P2A-mCherry

    Plasmid
    #198069
    Purpose
    Mammalian expression of iRFP713(C15S/V256C) and EGFP
    Depositor
    Michiyuki Matsuda
    Insert
    iRFP713(C15S/V256C)
    Use
    Tags
    P2A-mCherry
    Expression
    Mammalian
    Mutation
    C15S/V256C mutation
    Promoter
    CAG
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198069-plasmid-map-sequence-id-389877

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  16. pCA528 CHMP4B, residues 156-224, no Cys

    Plasmid
    #180641
    Purpose
    Bacterial expression for CHMP4B C-terminal tail, residues 156-224. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-140
    Depositor
    Wesley Sundquist
    Article
    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75
    Insert
    CHMP4B (CHMP4B Human)
    Use
    Tags
    HIS-SUMO
    Expression
    Bacterial
    Mutation
    Residues 156-224
    Promoter
    T7
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    180641-plasmid-map-sequence-id-358633

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  17. pCA528 CHMP4C, residues 156-233, no Cys

    Plasmid
    #180642
    Purpose
    Bacterial expression for CHMP4C C-terminal tail, residues 156-233. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-14.
    Depositor
    Wesley Sundquist
    Article
    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75
    Insert
    CHMP4C (CHMP4C Human)
    Use
    Tags
    HIS-SUMO
    Expression
    Bacterial
    Mutation
    residues 316-366
    Promoter
    T7
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    180642-plasmid-map-sequence-id-358646

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  18. pCA528 IST1, residues 315-360, no Cys

    Plasmid
    #180643
    Purpose
    Bacterial expression of IST1 C-terminal tail, residues 315-360. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-99.
    Depositor
    Wesley Sundquist
    Article
    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75
    Insert
    IST1 (IST1 Human)
    Use
    Tags
    HIS-SUMO
    Expression
    Bacterial
    Mutation
    residues 315-360
    Promoter
    T7
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    180643-plasmid-map-sequence-id-358585

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Showing: 2241 - 2260 of 5233 results