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pET-SUMO mCER-Prx2

Plasmid

#158226

Purpose

Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (Recombinant bacterial expression)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

mCER-Prx2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pHG4

Plasmid

#188252

Purpose

Integrating plasmid contains the TG1 int/attP locus and the apramycin-resistance gene (aac(3)IV) for selection

Depositor

Maggie Smith, Hong Gao

Article

Gao et al BMC Biotechnol. 2019 Jun 4;19(1):32. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

act1p/actII-orf4

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-PACT

Plasmid

#15667

Depositor

Dong-Yan Jin

Article

Kok et al J Biol Chem. 2007 Jun 15. 282(24):17649-

Insert

PACT (PRKRA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2007

Availability

Academic Institutions and Nonprofits only
pCMV5 Smad1-HA

Plasmid

#14956

Depositor

Joan Massague

Article

Liu et al Nature. 1996 Jun 13. 381(6583):620-3.

Insert

Smad1 (SMAD1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pJFRC7-UAS-NPRR_dTK

Plasmid

#133244

Purpose

Fluorescent reporter for neuropeptide release imaging

Depositor

David Anderson

Article

Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

Insert

drosophila tachykinin-fused codon-optimized GCaMP6s

Use

Tags

Expression

Insect

Mutation

Promoter

hsp70

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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RS314-SN

Plasmid

#17539

Depositor

Bert Vogelstein

Article

Thiagalingam et al Proc Natl Acad Sci U S A. 1995 Jun 20. 9

Insert

Gal1-p53 (TP53 Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
pCJH027_SC101_pLAC_LbCas12a_Kan

Plasmid

#183074

Purpose

Wild-type LbCas12a (Chen et al., 2018) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

LbCas12a

Use

Tags

Expression

Bacterial

Mutation

Wild-type

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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OA-1050E (Ubiq-CasRx)

Plasmid

#132416

Purpose

Express CasRx under Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

CasRx

Use

Tags

Expression

Insect

Mutation

Promoter

Ubiq

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp7

Plasmid

#163591

Purpose

Expresses BMP-7 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp7 (Bmp7 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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p221z-CAS9p-t35s

Plasmid

#118385

Purpose

Entry clone containing CAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

CAS9p

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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FRB-polySH33-EGFP

Plasmid

#175792

Purpose

Bacterial expression and purification, fluorescently tagged scaffold for droplet visualization and volume quantification

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

NCK1 (NCK1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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FRB-polySH35

Plasmid

#175234

Purpose

Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

NCK1 (NCK1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2D3

Plasmid

#15784

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2D3 (UBE2D3 Human)

Use

Tags

His

Expression

Bacterial

Mutation

see Depositor Comments below

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pMMB67EH-YFP

Plasmid

#90104

Purpose

YFP control expression plasmid

Depositor

Samuel Miller

Article

Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

Insert

YFP

Use

Broad-host range

Tags

Expression

Bacterial

Mutation

Promoter

Ptac

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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p255 pPr-CREM/Ins/CMV-STOP-luc

Plasmid

#8408

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

CREM/insulators/lox/luciferase

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

modified Cre

Promoter

Available since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 Orai3

Plasmid

#16370

Depositor

Anjana Rao

Article

Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

Insert

Orai3 (ORAI3 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pGEX-Skp2

Plasmid

#19946

Depositor

Yue Xiong

Article

Michel et al Cell Growth Differ. 1998 Jun . 9(6):435-

Insert

S-phase kinase-associated protein 2 (p45) (SKP2 Human)

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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pDropVn

Plasmid

#184873

Purpose

Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for Vibrio natriegens genome editing protocol.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-Cry2olig

Plasmid

#208780

Purpose

Inducibly expresses light activatable Cry2 domain

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Cry2 mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pEF-1α_ZF9LOV[C2]-VP64 (pLZA126)

Plasmid

#203911

Purpose

Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C2 position in mammalian cells

Depositor

Jared Toettcher

Article

Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

Insert

ZF9LOV[C2]-VP64

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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