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Plasmid#101813PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterSpy 1049Available sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shNup88-XbaI
Plasmid#87328PurposeTo express shRNA against human Nup88DepositorInsertshRNA against human Nup88
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shNup93-XhoI
Plasmid#87330PurposeTo express shRNA against human Nup93DepositorInsertshRNA against human Nup93
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
T7-VEE-GFP
Plasmid#58977Purposeexpression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA repliconDepositorInsertEGFP
UseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoter26S subgenomic promoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
Lapronic/pcDNA3.1(-)
Plasmid#140756PurposeFRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate RatioDepositorInsertLapronic (Lactate/PYRuvate Ratio Optical Nano-Indicator from CECs)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
M50 Super 8x TOPFlash
Plasmid#12456PurposeBeta-catenin reporter. TCF/LEF sites upstream of a luciferase reporter.DepositorInsertTCF/LEF binding sites (Ctnnb1 )
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
pIRES:hTRAAK
Plasmid#133080PurposeMammalian expression vector. It will generate the human TRAAK channel full lengthDepositorInsertPotassium channel subfamily K member 4 (KCNK4, TRAAK) (KCNK4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synapsin-HaloCaMP1a-EGFP
Plasmid#138327Purposechemigenetic calcium indicatorDepositorHas ServiceAAV1 and AAV9InsertHaloCaMP1a
UseAAVTagsEGFP, His-tag, and Nuclear Exclusion SignalExpressionMammalianMutationPromotersynapsinAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-synapsin-HaloCaMP1b-EGFP
Plasmid#138328Purposechemigenetic calcium indicatorDepositorHas ServiceAAV1 and AAV9InsertHaloCaMP1b
UseAAVTagsEGFP, His-tag, and Nuclear Exclusion SignalExpressionMammalianMutationPromotersynapsinAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKRaION1-N1
Plasmid#177801PurposeMammalian expression of green fluorescent potassium indicator KRaION1DepositorInsertKRaION1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX307-VHL-3F-BirA
Plasmid#220147PurposeExpresses VHL-BirA for VHL substrate identification by E-STUBDepositorInsertvon Hippel-Lindau tumor suppressor (VHL Human)
UseLentiviralTags3F-BirAExpressionMammalianMutationcodon optimizedPromoterEF-1aAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKESaPar
Plasmid#211829PurposeLow copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.DepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterT5Available sinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVP91A-pcaHG
Plasmid#113766PurposeExpresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.DepositorInsertsβ subunit of Protocatechuate 3,4- Dioxygenase
α subunit of Protocatechuate 3,4- Dioxygenase
UseTagsMet-6xHisExpressionBacterialMutationPromoterT5 promoterAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
CROP-sgRNA-MS2
Plasmid#153457PurposeCROP-seq vector with mCherry and x2 MS2 loops in gRNA scaffoldDepositorInsertsgRNA empty backbone
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKERepPar
Plasmid#211827PurposeMedium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.DepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterT5Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV_nLightG
Plasmid#217656PurposeExpresses norepinephrine indicator nLightG under mammalian promoterDepositorInsertnLightG
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
p11d-tRPA (MSW#111)
Plasmid#208066Purposeexpression of human Replication Protein A in E. coliDepositorUseTagsExpressionBacterialMutationRPA32 has a point mutation Thr88(ACC) -> Ile(A…PromoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
piGECI-N1
Plasmid#160421PurposeCytoplasmic expression of a near-infrared genetically encoded calcium indicator iGECIDepositorInsertiGECI
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -