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Plasmid#175784PurposePlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsAviTag, Hexahistidine tag, and eGFPExpressionBacterialMutationE184QPromoterT7Available sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 aa 1-239
Plasmid#74609PurposeExpresses Hook domain and first coiled coil of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-239 (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFlag-CMV2-Brd4 R1337P
Plasmid#21939DepositorInsertBRD4 (BRD4 Human)
UseTagsFlagExpressionMammalianMutationaa 1209-1362 only, R1337PPromoterAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFlag-CMV2-Brd4 S1351R
Plasmid#21941DepositorInsertBRD4 (BRD4 Human)
UseTagsFlagExpressionMammalianMutationaa1209-1362 only, S1351RPromoterAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pFlag-CMV2-Brd4 D1346N
Plasmid#21940DepositorInsertBRD4 (BRD4 Human)
UseTagsFlagExpressionMammalianMutationaa 1209-1362 only, D1346NPromoterAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
416-Met25 Kar2 printor HDEL
Plasmid#32129DepositorInsertPrintor (KLHL14 Human)
UseTagsHDEL and Kar2ExpressionYeastMutationPromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
p3 FLAG_CMV14/OCTN2 (wt)
Plasmid#112799PurposeExpression of OCTN2DepositorInsertOCTN2/SLC22A5 (Slc22a5 Rat)
UseTags3 x FlagExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pET28a-Hook3 aa 1-160
Plasmid#74607PurposeExpresses Hook domain of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-160 (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQE9-His-p97deltaD2(K251A)
Plasmid#17230DepositorInsertp97 (Vcp Mouse)
UseTagsHisExpressionBacterialMutationchange codon 457 to stop codon, change lysine 25…PromoterAvailable sinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
TAK11
Plasmid#227095PurposeLuciferase reporter vector with HIV-1 TAR region cloned in pGL3 Basic vectorDepositorInsertCMV Promoter and HIV-1 TAR region
UseLuciferaseTagsExpressionMutationPromoterCMVAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
TAK8
Plasmid#227096PurposeLuciferase reporter vector with wild type 5' Element (MMTV) insertDepositorInsertCMV Promoter and R to 400bp Gag
UseLuciferaseTagsExpressionMutationPromoterCMVAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMamCol1
Plasmid#215184PurposeAllows for stable expression of recombinant (tagged/untagged) proteins in AAVS1 locus of Human cells and transient Protein Expression in E. coliDepositorInserttsPurple
UseAAV, CRISPR, and Synthetic Biology ; Recombinant …TagsSNAC-StrepII tagExpressionBacterial and MammalianMutationPromoterTetON 3G Bidirectional for Mammalian and Trc with…Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEK24
Plasmid#210597PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdACTINpro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastMutationPromoterBdACTINproAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-TdIF2
Plasmid#196906Purposevisualization of TdIF2/ERBP as a fusion to mCherryDepositorInsertTdIF2
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-TdIF2
Plasmid#199699Purposevisualization of nucleolus in living mammalian cellsDepositorInsertTdIF2
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Linked Free NES (M)
Plasmid#182436PurposeYeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS(M)-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free NES (M)
Plasmid#182437PurposeYeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS(M)
NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10 and GAL7Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPLN-hFluc-WT-eGFP-KDEL
Plasmid#177727PurposeFirefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminusDepositorInserthFluc
UseTagsKDEL, Prolactin Signal Sequence, and eGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only