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Plasmid#162728PurposeMammalian expressionDepositorInsertFKBP F36V
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFS_1112_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23109-Leader-ARRAY2-DR2-FaqI_Term_NotI
Plasmid#184740Purposeencodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23109DepositorInsertFsRT-Cas1(opt)-Cas2(opt)
UseTagsExpressionBacterialMutationsequence codon optimized for expression in E. coliPromoterpTetAAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
mKikGR-Tubulin-6
Plasmid#57330PurposeLocalization: Microtubules, Excitation: 507 / 583, Emission: 517 / 593DepositorInsertTubulin (TUBA1B Human)
UseTagsmKikGRExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-HtrA2-FLAG S142D
Plasmid#15939DepositorInsertHtrA2 (HTRA2 Human)
UseTagsFLAGExpressionMammalianMutationS142DPromoterAvailable sinceNov. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-MKLP1 S708/710A
Plasmid#70153Purposemammalian expression of MKLP1 with an eGFP fusion and S708A/S710A mutationsDepositorInsertMKLP1 (KIF23 Human)
UseTagseGFPExpressionMammalianMutationS708A, S710APromoterCMVAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET20b-delta133HtrA2 G399S
Plasmid#16158DepositorInsertHtrA2 (HTRA2 Human)
UseTagsHisExpressionBacterialMutationG399SPromoterT7Available sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pX330 LC-FKBP-Cas9
Plasmid#162724PurposeMammalian expressionDepositorInsertFKBP F36V
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1 hSlp4-a del.SHD
Plasmid#40067DepositorInserthSlp4-a V21A (SYTL4 Human)
UseTagsGSTExpressionBacterialMutationdeleted SHD domain (1-143)PromoterTacAvailable sinceOct. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-o-R179C-EE
Plasmid#55780PurposeThis is a constitutively activated G protein alpha-o mutant containing an internal EE epitope.DepositorInsertG protein alpha-o with constitutively activating R179C mutation (Gnao1 Rat)
UseTagsEE epitope was introduced internally with D167E a…ExpressionBacterial and MammalianMutationArginine 179 was replaced with cysteinePromoterCMVAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDSHA-ARL6-Myc
Plasmid#67359PurposeMammalian expression of hArl6 with C-term Myc tagDepositorInsertARL6 (ARL6 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-MKLP1 S708A
Plasmid#70146Purposemammalian expression of MKLP1 with an eGFP fusion and an S708A mutationDepositorInsertMKLP1 (KIF23 Human)
UseTagseGFPExpressionMammalianMutationS708APromoterCMVAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWzl_neo_DEST_flag_PIK3CA
Plasmid#45308DepositorInsertPIK3CA (PIK3CA Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL6-V5-His6
Plasmid#67422PurposeExpression of hArl6 with C-term V5 and His6 tag in E. coliDepositorInsertARL6 (ARL6 Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
HTRA2_PDZ_1
Plasmid#103913PurposeProtein expression and purification of HTRA2 PDZ domainDepositorInsertHTRA2 (HTRA2 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK-FLAG-TRAF4(K190R)
Plasmid#58317PurposeFLAG-tagged mouse TRAF4 K190R mutantDepositorInsertTNF receptor associated factor 4 (Traf4 Mouse)
UseTagsFLAGExpressionMammalianMutationK 190 to RPromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1 hSlp4-a V21A
Plasmid#40064DepositorInserthSlp4-a V21A (SYTL4 Human)
UseTagsGSTExpressionBacterialMutationValine 21 to AlaninePromoterTacAvailable sinceOct. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
mCherry-miniSOG-Tubulin-6
Plasmid#55097PurposeLocalization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertTubulin (TUBA1B Human)
UseTagsmCherry-miniSOGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-MKLP1 S710A
Plasmid#70149Purposemammalian expression of MKLP1 with an eGFP fusion and an S710A mutationDepositorInsertMKLP1 (KIF23 Human)
UseTagseGFPExpressionMammalianMutationS710APromoterCMVAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL6-no stop
Plasmid#67329PurposehArl6 without stop codon in Gateway Entry vectorDepositorInsertARL6 (ARL6 Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-ARL6-V5-His6
Plasmid#67443PurposeMammalian expression of hArl6 with C-term V5 and His6 tagDepositorInsertARL6 (ARL6 Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only