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pJW1354
Plasmid#69490PurposeGly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology armsDepositorInsertGSGGGG spacer-Degron-TEV protease site-3x FLAG epitope
UseCRISPR; Template to generate cassettes for -media…TagsExpressionMutationPromoterAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYES2-HTH-HHT2
Plasmid#86482Purposeyeast expression of HHT2 with His10-TEV-HA tagDepositorInsertHHT2 (HHT2 Budding Yeast)
UseTagsHIS10-TEV-HA (HTH)ExpressionYeastMutationPromoterGAL1Available sinceJan. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ubp8/PET32a
Plasmid#26965DepositorInsertUbp8 (UBP8 Budding Yeast)
UseTagsHis6 and TrxExpressionBacterialMutationTEV site is added t the 5' end and 4 alanine…PromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRC05
Plasmid#114684PurposeFKBP-TEV-CDepositorInsertFKBP-TEV-C
UseTagsGSTExpressionBacterialMutationPromoterT7 PromoterAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRC04
Plasmid#114683PurposeFRB-TEV-NDepositorInsertFRB-TEV-N
UseTagsMBPExpressionBacterialMutationPromoterTac PromoterAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
L3MBTL3 (3UT1)
Plasmid#36901PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertL3MBTL3 (L3MBTL3 Human)
UseTagsHis tag and Streptavidin-Binding Peptide (SBP)-Ta…ExpressionBacterialMutationcontains amino acid residues 228-551 onlyPromoterAvailable sinceJune 21, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCEP13
Plasmid#207584PurposeTolQR expression vector for purification (TolQ-TolR-TEV-Strep_His)DepositorInsertTolQ-TolR-TEV-Strep_His
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
Atg24 1B
Plasmid#197046PurposeHis6 tagged Atg24 from S. cerevisiae for expression in E. coli.DepositorInsertAtg24
UseTags6His-TEVExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
Atg32 [200-341] 1B
Plasmid#190431PurposeRecombinant expression plasmid of S. cerevisiae Atg32 amino acids 200 - 341 in E. coli.DepositorInsertAtg32
UseTags6xHis-TEVExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 H237A-EGFP
Plasmid#201425PurposeBacterial protein expression of Dcp2 H237ADepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsEGFP, TEV, 6xHis and MBP, TEVExpressionBacterialMutationH237APromotertacAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
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Dcp1 H206A-EGFP
Plasmid#201424PurposeBacterial protein expression of Dcp1 H206ADepositorInsertDcp1 (DCP1 Budding Yeast)
UseTagsEGFP, TEV, 6xHis and MBP, TEVExpressionBacterialMutationH206APromotertacAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
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Dcp1 H198A-EGFP
Plasmid#201423PurposeBacterial protein expression of Dcp1 H198ADepositorInsertDcp1 (DCP1 Budding Yeast)
UseTagsEGFP, TEV, 6xHis and MBP, TEVExpressionBacterialMutationH198APromotertacAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dcp1 H43A-EGFP
Plasmid#201422PurposeBacterial protein expression of Dcp1 H43ADepositorInsertDcp1 (DCP1 Budding Yeast)
UseTagsEGFP, TEV, 6xHis and MBP, TEVExpressionBacterialMutationH43APromotertacAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
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Dcp1 H40A-EGFP
Plasmid#201421PurposeBacterial protein expression of Dcp1 H40ADepositorInsertDcp1 (DCP1 Budding Yeast)
UseTagsEGFP, TEV, 6xHis and MBP, TEVExpressionBacterialMutationH40APromotertacAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -