Addgene

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pET28a-matNODALvar-HIS

Plasmid

#115265

Purpose

For bacterial recombinant protein production of the mature peptide of a human NODAL splice variant

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL variant mature peptide (NODAL Human)

Use

Tags

6His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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NODALvar-MYC-DYK

Plasmid

#115245

Purpose

For mammalian expression of a human NODAL splice variant open reading frame with a C-terminal MYC-DYK tag

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Use

Tags

MYC-DYK

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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NODALvar-matMYC

Plasmid

#115249

Purpose

For mammalian expression of a human NODAL splice variant open reading frame with an internal MYC tag

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL splice variant (NODAL Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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NODALvar-T7TS

Plasmid

#115251

Purpose

For in vitro transcription of a human NODAL splice variant open reading frame RNA

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL splice variant (NODAL Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pET-His6-Sumo-CylK-Strep

Plasmid

#185962

Purpose

Expresses His6-Sumo-CylK-Strep in E. coli for refolding protein preparation

Depositor

Emily Balskus

Article

Braffman et al Elife. 2022 Feb 25;11. pii: 75761. doi:

Insert

CylK

Use

Tags

His6-Sumo

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-Bighead S-FC

Plasmid

#180147

Purpose

mRNA synthesis for injection into embryos

Depositor

Edward De Robertis

Article

Ding et al Proc Natl Acad Sci U S A. 2018 Sep 25;11

Insert

Bighead.S

Use

Tags

FC

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2 Bighead S-HA

Plasmid

#172485

Purpose

for synthesis of mRNA for injection into xenopus laevis embryos

Depositor

Edward De Robertis

Article

Ding et al Proc Natl Acad Sci U S A. 2018 Sep 25;11

Insert

Bighead.S

Use

In vitro mrna synthesis

Tags

HA and S-HA

Expression

Mutation

Promoter

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-xFAM3b.L-HA

Plasmid

#172852

Purpose

mRNA synthesis for injection into embryos

Depositor

Edward De Robertis

Article

Ding et al Proc Natl Acad Sci U S A. 2018 Sep 25;11

Insert

xFAM3B.L (fam3b.L Frog)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1

Plasmid

#145369

Purpose

Gateway destination vector for cell-free and mamalian expression with N-term 8xHis and eGFP tag

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Type

Empty backbone

Use

Tags

His and eGFP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace1_Q89D_D100N_E206V-HaloTag

Plasmid

#129255

Purpose

Express Ace1_Q89D_D100N_E206V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace1_Q89D_D100N_E206V-HaloTag

Use

Tags

Expression

Mammalian

Mutation

Q89D, D100N, E206V

Promoter

CAG

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Mac_Q139D_D150N_E258V-HaloTag-ST

Plasmid

#129264

Purpose

Express soma-localized Mac_Q139D_D150N_E258V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Mac_Q139D_D150N_E258V-HaloTag-ST

Use

Tags

Expression

Mammalian

Mutation

Q139D,D150N,E258V

Promoter

CAG

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-QuaAr3_Q95D_H106N_E214V-HaloTag-ST

Plasmid

#129263

Purpose

Express soma-localized QuaAr3_Q95D_H106N_E214V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

QuaAr3_Q95D_H106N_E214V-HaloTag-ST

Use

Tags

Expression

Mammalian

Mutation

Q95D, H106N, E214V

Promoter

CAG

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace1_Q89D_D100N_E206V-HaloTag-ST

Plasmid

#129260

Purpose

Express soma-localized Ace1_Q89D_D100N_E206V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace1_Q89D_D100N_E206V-HaloTag-ST

Use

Tags

Expression

Mammalian

Mutation

Q89D, D100N, E206V

Promoter

CAG

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Mac_Q139D_D150N_E258V-HaloTag

Plasmid

#129259

Purpose

Express Mac_Q139D_D150N_E258V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Mac_Q139D_D150N_E258V-HaloTag

Use

Tags

Expression

Mammalian

Mutation

Q139D,D150N,E258V

Promoter

CAG

Available since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-QuaAr3_Q95D_H106N_E214V-HaloTag

Plasmid

#129258

Purpose

Express QuaAr3_Q95D_H106N_E214V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

QuaAr3_Q95D_H106N_E214V-HaloTag

Use

Tags

Expression

Mammalian

Mutation

Q95D, H106N, E214V

Promoter

CAG

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH7-8

Plasmid

#124991

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH7-8 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH7-8 TCRa P2A TCRb

Use

retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH7-11

Plasmid

#124993

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH7-11 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH7-11 TCRa P2A TCRb

Use

retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH7-9

Plasmid

#124992

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH7-9 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH7-9 TCRa P2A TCRb

Use

retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH7-6

Plasmid

#124990

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH7-6 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH7-6 TCRa P2A TCRb

Use

retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH5-4

Plasmid

#124984

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH5-4 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH5-4 TCRa P2A TCRb

Use

retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge