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Showing: 2221 - 2240 of 2425 results

  2. pHLmMBP-2

    Plasmid
    #72344
    Purpose
    Mammalian expression of secreted N-terminally 6His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Use
    Tags
    6His-tag, HA-tag, Strep-tag II, and mMBP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72344-plasmid-map-sequence-id-132067

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  3. pJZC78

    Plasmid
    #62339
    Purpose
    sgRNA + 1x COM with COM-KRAB effector for mammalian cells
    Depositor
    Wendell Lim, Stanley Qi
    Article
    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01
    Inserts
    sgRNA + 1x COM binding module
    COM-KRAB
    Use
    Lentiviral
    Tags
    KRAB
    Expression
    Mammalian
    Mutation
    Targets sv40 promoter, sequence: CATACTTCTGCCTGCT…
    Promoter
    CMV and U6
    Available since
    April 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    62339-plasmid-map-sequence-id-102262

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  4. pQ54-VC

    Plasmid
    #175060
    Purpose
    expresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ54) in mammalian cells
    Depositor
    Arnold Boersma
    Article
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Insert
    polyQ54 (HTT Human)
    Use
    Tags
    mVenus + mCherry
    Expression
    Mammalian
    Mutation
    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…
    Promoter
    CMV Promoter
    Available since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175060-plasmid-map-sequence-id-353799

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  5. pQ25-VC

    Plasmid
    #175062
    Purpose
    expresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ25) in mammalian cells
    Depositor
    Arnold Boersma
    Article
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Insert
    polyQ25 (HTT Human)
    Use
    Tags
    mVenus + mCherry
    Expression
    Mammalian
    Mutation
    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…
    Promoter
    CMV Promoter
    Available since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175062-plasmid-map-sequence-id-362344

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  6. pHLmMBP-1

    Plasmid
    #72343
    Purpose
    Mammalian expression of secreted mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Use
    Tags
    6His-tag, HA-tag, Strep-tag II, and mMBP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72343-plasmid-map-sequence-id-132066

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  7. pFUS-WT-VC

    Plasmid
    #175068
    Purpose
    expresses FUS wildtype tagged to mVenus and mCherry in mammalian cells
    Depositor
    Arnold Boersma
    Article
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Insert
    FUS glycine rich protein (FUS Human)
    Use
    Tags
    HA and mVenus + mCherry
    Expression
    Mammalian
    Mutation
    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…
    Promoter
    CMV Promoter
    Available since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175068-plasmid-map-sequence-id-353791

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  9. pQ39-VC

    Plasmid
    #175061
    Purpose
    expresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ39) in mammalian cells
    Depositor
    Arnold Boersma
    Article
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Insert
    polyQ39 (HTT Human)
    Use
    Tags
    mVenus + mCherry
    Expression
    Mammalian
    Mutation
    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…
    Promoter
    CMV Promoter
    Available since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175061-plasmid-map-sequence-id-353797

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  11. pBrain-GFP-TACC3KDP(L566,567A)-shTACC3

    Plasmid
    #69117
    Purpose
    Dual-promoter plasmid to express knockdown-proof human TACC3 (L566A and L567A substitutions) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.
    Depositor
    Stephen Royle
    Article
    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:
    Inserts
    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    shTACC3
    Use
    RNAi
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    LL (566-567aa) mutated to AA. Silent mutations i…
    Promoter
    CMV
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    69117-plasmid-map-sequence-id-122092

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Showing: 2221 - 2240 of 2425 results