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Plasmid#79490PurposeMultiSite Gateway entry clones, first fragment, (attL1, attR5) CAG promoterDepositorInsertCAG Promoter
UseMultisite gateway entry cloneTagsExpressionMutationPromoterCAG PromoterAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-3P2A
Plasmid#111772PurposeQuadcistronic construct: Co-express four genes by 3 P2A peptidesDepositorTypeEmpty backboneUseCloning intermediateTagsExpressionMutationPromoterT7Available sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-mCherry
Plasmid#79509PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) mCherry RFP CDSDepositorInsertmCherry RFP
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4a G12D
Plasmid#83168PurposeGateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G12D mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationG12DPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-mKate2-P2A-TurboID-NS
Plasmid#166568PurposeMultisite Gateway middle entry clone encoding TurboID with mKate2 marker. Parton lab clone IYNDepositorInsertmKate2-P2A-TurboID
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS79 (eft-3p::Cas9 + sgRNA)
Plasmid#154839PurposeCas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGGDepositorInsertsgRNA
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-mVenus
Plasmid#67719PurposeMultisite gateway entry clone for adding C-terminal fusions of mVenus (no ATG)DepositorInsertmVenus
UseGateway multisite 3' entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BsaI
Plasmid#128649PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCoofy50
Plasmid#55189PurposeMammalian expression vector for parallel SLIC cloning containing N-terminal HA, 8x His and FLAG tags. C-terminal tags can be added during SLIC.DepositorTypeEmpty backboneUseTags8x His, FLAG, HA, PreScission site (3C protease s…ExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4a G12V
Plasmid#83169PurposeGateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G12V mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationG12VPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
[GG][Pin]
Plasmid#97191PurposePin nuclease domain with Pum cloning siteDepositorInsertPin domain
UseSynthetic BiologyTagsPum cloning siteExpressionMammalianMutationPromoterCMVAvailable sinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4a G12C
Plasmid#83167PurposeGateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G12C mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationG12CPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b G12S
Plasmid#83144PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12S mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationG12SPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti_TMv2
Plasmid#131761Purposeframeshift reporter for barcode retrievalDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-SapI
Plasmid#128599PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BsmBI
Plasmid#128648PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-Renilla
Plasmid#79496PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Renilla Luciferase CDSDepositorInsertRenilla Luciferase
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b S17N
Plasmid#83156PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), S17N mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationS17NPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-Firefly
Plasmid#79495PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Firefly Luciferase CDSDepositorInsertFirefly Luciferase
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-PAmCherry1_160-236-MCS
Plasmid#60546PurposeCloning PAmCherry1 C-terminal fragment (RC) for BiFC-PALMDepositorInsertPAmCherry1 C-terminal fragment
UseCloningTagsExpressionMutationamino acids 160-236PromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only