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Plasmid#138586PurposeExpress EGFP with an upstream T7 promoter in pUC19 backboneDepositorInsertEGFP
UseTagsExpressionMutationPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMuLE Lenti Dest eGFP
Plasmid#62175PurposeMuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses EGFP under the control of a PGK promoter. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseLentiviral; Mule gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Rac1-Q61L
Plasmid#12981DepositorInsertRac1 constitutively active (RAC1 Human)
UseTagsEGFPExpressionMammalianMutationQ61L Constitutively activePromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
rLAMP1-mCherry-EGFP-Tet-On
Plasmid#109015PurposeTet-On construct to induce expression of tandem eGFP-mCherry tagged mLAMP1 (lysosome marker)DepositorInsertrLAMP1
UseLentiviralTagsmcherry-eGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGTag-NLS-eGFP-SV40
Plasmid#117811PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInserteGFP
UseSynthetic BiologyTagsNLSExpressionMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EGFP-IRES-Neo
Plasmid#128660Purposemammalian expression of EGFP with neomycin selectionDepositorInsertEGFP
UseLentiviralTagsN/AExpressionMammalianMutationWild typePromoterCMVAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOTTC407 - pAAV EF1a eGFP
Plasmid#60058PurposeAn AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.DepositorInsertenhanced Green Fluorescent Protein
UseAAVTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN1-human Dynamin 1aa
Plasmid#22163DepositorInserthuman Dynamin 1aa (DNM1 Human)
UseTagsGFPExpressionBacterial and MammalianMutationD744NPromoterAvailable sinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCW-eGFP-FHA-SHLD2
Plasmid#114121PurposeLentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2; 53BP1-independent recruitment to damaged chromatinDepositorInsertSHLD2 (SHLD2 Human)
UseLentiviral; Doxycycline inducibleTagsRNF8 FHA domain (aa 2-160) and eGFPExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceAug. 28, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDH-EF1a-eFFly-eGFP
Plasmid#104834PurposeeFFly luciferase for invivo Imaging, eGFP for flourescent detectionDepositorInsertseFFly
eGFP
UseLentiviral and Luciferase; EgfpTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCW-eGFP-FHA-SHLD2-m1
Plasmid#114122PurposeLentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2, m1 mutant; 53BP1-independent recruitment to damaged chromatinDepositorInsertSHLD2 (m1 mutant) (SHLD2 Human)
UseLentiviral; Doxycycline inducibleTagsRNF8 FHA domain (aa 2-160) and eGFPExpressionMammalianMutationW489A, F494A, W495A mutations introducedPromoterTRE promoter, Tet ONAvailable sinceAug. 28, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG dCas9-KRAB-2A-EGFP
Plasmid#92396PurposeCAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.DepositorInsertdCas9_KRAB_2A_EGFP
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-eGFP-53BP1
Plasmid#60813Purposemammalian expression vectorDepositorInsert53BP1 (TP53BP1 Human)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceDec. 9, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1_miniSOG2 T2A H2B-EGFP
Plasmid#87410PurposeMammalian expression vector encoding Histone 2B GFP fusion and miniSOG2DepositorInsertmini singlet oxygen generator 2
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Cdc42-T17N
Plasmid#12976DepositorInsertCdc42 DN (CDC42 Human)
UseTagsEGFPExpressionMammalianMutationT17N Dominant negativePromoterCMVAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Cdc42-Q61L
Plasmid#12986DepositorInsertCdc42 constitutively active (CDC42 Human)
UseTagsEGFPExpressionMammalianMutationQ61L Constitutively activePromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-dCas9-MQ1(WT)-EGFP
Plasmid#89633PurposeTargeted CpG methylationDepositorInsertsite-specific DNA-methyltransferase SssI
UseCRISPRTags3*FLAG and 6*HisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-IRES eGFP
Plasmid#128059PurposeLentiviral expression of an insert from EF1 promoter and co-expression of EGFP. See Depositor Comments for additional information regarding plasmid sequence.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Rac1-T17N
Plasmid#12982DepositorInsertRac1 DN (RAC1 Human)
UseTagsEGFPExpressionMammalianMutationT17N Dominant negativePromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-3XMyc-EGFP-PEX26
Plasmid#139059PurposeMyc-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.DepositorInsert3XMyc-EGFP-PEX26
UseLentiviralTagsMycExpressionMutationPromoterUBCAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only