Addgene

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pMSCV-p18Ink4c-IRES-GFP

Plasmid

#52220

Purpose

retroviral bicistronic expression of p18Ink4c and GFP

Depositor

Martine Roussel

Article

Forget et al Cell Cycle. 2008 Dec;7(23):3737-46. Epub

Insert

Ink4c (Cdkn2c Mouse)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1+_LN-HA-AGO2-5xE

Plasmid

#92011

Purpose

Expresses Lambda-N-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Tags

Lambda-N-HA

Expression

Mammalian

Mutation

S824E, S828E, T830E, S831E, S834E (5xE)

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO-ELF1

Plasmid

#98627

Purpose

Gateway entry TOPO TA cloning vector ELF1

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ELF1 (Elf1 Mouse)

Use

Topo ta cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF4C03

Plasmid

#70961

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

His2Av (His2Av Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only
pLCHKO_TK1_HPRT1_Lb_4

Plasmid

#155062

Purpose

Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a (CHyMErA system)

Depositor

Jason Moffat, Benjamin Blencowe

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

(hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pMT2 Alpha2 delta-1 V6

Plasmid

#89894

Purpose

Mammalian expression of Alpha2 delta-1 V6

Depositor

Annette Dolphin

Article

Kadurin et al Elife. 2016 Oct 26;5. pii: e21143. doi:

Insert

Cacna2d1 (Cacna2d1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Amino acids L943V, E944V, A945V, E947V, M948V are…

Promoter

adenovirus major late promoter

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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BAM3 X078_pECIA2

Plasmid

#115106

Purpose

Bait vector BAM3 X078_pECIA2 should be used with prey vector BAM3 X078_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT4G20270 (AT4G27020 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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RGFR3 X052_pECIA2

Plasmid

#115107

Purpose

Bait vector RGFR3 X052_pECIA2 should be used with prey vector RGFR3 X052_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT4G26540 (AT4G26540 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT4G22730 K11_pECIA2

Plasmid

#115024

Purpose

Bait vector AT4G22730 K11_pECIA2 should be used with prey vector AT4G22730 K11_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT4G22730 (AT4G22730 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT4G23740 J11_pECIA2

Plasmid

#114998

Purpose

Bait vector AT4G23740 J11_pECIA2 should be used with prey vector AT4G23740 J11_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT4G23740 (AT4G23740 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT2G37050 N3_pECIA2

Plasmid

#114952

Purpose

Bait vector AT2G37050 N3_pECIA2 should be used with prey vector AT2G37050 N3_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G37050 (AT2G37050 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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RGFR3 X052_pECIA14

Plasmid

#114907

Purpose

Prey vector RGFR3 X052_pECIA14 should be used with bait vector RGFR3 X052_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT4G26540 (AT4G26540 Mustard Weed)

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT4G22730 K11_pECIA14

Plasmid

#114824

Purpose

Prey vector AT4G22730 K11_pECIA14 should be used with bait vector AT4G22730 K11_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT4G22730 (AT4G22730 Mustard Weed)

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT4G23740 J11_pECIA14

Plasmid

#114798

Purpose

Prey vector AT4G23740 J11_pECIA14 should be used with bait vector AT4G23740 J11_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT4G23740 (AT4G23740 Mustard Weed)

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT2G37050 N3_pECIA14

Plasmid

#114752

Purpose

Prey vector AT2G37050 N3_pECIA14 should be used with bait vector AT2G37050 N3_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G37050 (AT2G37050 Mustard Weed)

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pDONOR-SNCAe3-WT

Plasmid

#85847

Purpose

Donor plasmid for SNCA exon3 wild type sequence. lso contains TagBFP and dTomato

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

SNCA exon 3 homology arms (SNCA Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_Puro_SFB_GNB1L

Plasmid

#224381

Purpose

Lenti plasmid for generating GNB1L expressing stable cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pX459_TELO2_gRNA

Plasmid

#224379

Purpose

Targets TELO2 for dTAG N terminal knock-in cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

TELO2 (TELO2 Human)

Use

CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pX459_GNB1L_gRNA

Plasmid

#224377

Purpose

Targets GNB1L for dTAG C terminal knock-in cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Use

CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCHMP3-mut-NS3-Green

Plasmid

#223530

Purpose

Mammalian expression of CHMP3 fused to the hepatitis C virus protease NS3 through a short linker containing mutated NS3 cleavage site, along with a green fluorescent protein.

Depositor

Winfried Weissenhorn

Article

Wang et al Viruses. 2023 Nov 22;15(12):2289. doi: 1

Insert

CHMP3 (CHMP3 Human)

Use

Tags

NS3 Hepatitis C protease and mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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