Addgene

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M3939 leu2::ADE2 Disruptor Converter

Plasmid

#51672

Purpose

Yeast marker swap plasmid for converting LEU2 to ADE2

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

ADE2 (ADE2 Budding Yeast)

Use

Yeast marker swap

Tags

Expression

Yeast

Mutation

Promoter

ADE2

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pFP125

Plasmid

#21674

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

LacZ (lacZ E. coli)

Use

Centromeric plasmid

Tags

Expression

Mutation

The second lacZ sequence has been completely dele…

Promoter

Available since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
RFP->M71-IRES-M71-IRES-tauGFP ACNF TV

Plasmid

#15629

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):833-46.

Insert

RFP->M71-IRES-M71-IRES-tauGFP ACNF TV

Use

High copy number

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pT2U2-YFP

Plasmid

#84544

Purpose

Hybrid reporter construct with 4 transcription factor binding sites (2 TetO, 2 UAS) driving EYFP

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

T2U2-EYFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pT2U3-YFP

Plasmid

#84547

Purpose

Hybrid reporter construct with 5 transcription factor binding sites (2 TetO, 3 UAS) driving EYFP

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

T2U3-EYFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pU2T4-YFP

Plasmid

#84548

Purpose

Hybrid reporter construct with 6 transcription factor binding sites (2 UAS, 4 TetO) driving EYFP

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

U2T4-EYFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pT2U2T2-YFP

Plasmid

#84549

Purpose

Hybrid reporter construct with 6 transcription factor binding sites (2 TetO, 2 UAS, 2 TetO) driving EYFP

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

T2U2T2-EYFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pU2T4U2-YFP

Plasmid

#84551

Purpose

Hybrid reporter construct with 8 transcription factor binding sites (2 UAS, 4 TetO, 2 UAS) driving EYFP

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

U2T4U2-EYFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pT2U4T2-YFP

Plasmid

#84553

Purpose

Hybrid reporter construct with 8 transcription factor binding sites (2 TetO, 4 UAS, 2 TetO) driving EYFP

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

T2U4T2-EYFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pU2T2-YFP

Plasmid

#84543

Purpose

Hybrid reporter construct with 4 transcription factor binding sites (2 UAS, 2 TetO) driving EYFP

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

U2T2-EYFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pM2P20X

Plasmid

#108210

Purpose

Expresses MSePCNA2-PdX fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

MsePCNA1-PdX fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pS3C

Plasmid

#108211

Purpose

Expresses SsoPCNA3-CYP101A1 fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

SsoPCNA3-CYP101A1 fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pM3C

Plasmid

#108212

Purpose

Expresses MsePCNA3-CYP101A1 fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

MsePCNA3-CYP101A1 fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pM1R

Plasmid

#108208

Purpose

Expresses MsePCNA1-PdR fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

MsePCNA1-PdR fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pS1R

Plasmid

#108207

Purpose

Expresses SsoPCNA1-PdR fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

SsoPCNA1-PdR fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P81nmt1-mECitrine

Plasmid

#105153

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Davidson et al PLoS One. 2016 Nov 29;11(11):e0167043. d

Insert

kanMX6-P81nmt1-mECitrine

Use

Tags

81nmt1-mECitrine

Expression

Bacterial and Yeast

Mutation

F64L, Q69M, A206K

Promoter

81nmt1

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCri-4a-Strep

Plasmid

#61338

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Use

Tags

His-tag, Strep-tag, and TRX-tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pCri-1a-Strep

Plasmid

#61337

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Use

Tags

His-tag and MBP-tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pMSCV-MAVS-M1A(KaganE18)

Plasmid

#52009

Purpose

Expresses the human MAVS CDS containing the point mutation M1A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M1A

Use

Retroviral

Tags

Expression

Mammalian

Mutation

changed Met 1 to Ala

Promoter

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pMSCV-MAVS-M142A(KaganE19)

Plasmid

#52010

Purpose

Expresses the human MAVS CDS containing the point mutation M142A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M142A

Use

Retroviral

Tags

Expression

Mammalian

Mutation

changed Met 142 to Ala

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only