Addgene

We narrowed to 3,954 results for: chm

Showing: 2201 - 2220 of 3954 results

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pAc5.1B-EGFP-DmNot10

Plasmid

#79254

Purpose

Expresses EGFP-tagged DmNot10 in S2 cells

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot10 (Not10 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Expression

Mutation

Promoter

Ac5 promoter

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot11

Plasmid

#79253

Purpose

Expresses EGFP-tagged DmNot11 in S2 cells

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot11 (Not11 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Expression

Mutation

Promoter

Ac5 promoter

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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PCBP4_3xKH_pGEX

Plasmid

#135156

Purpose

Recombinant expression of PCBP4 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

PCBP4 (PCBP4 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pDAS12137_sgRNA-PEAR-GFP-nick(+17)-mCherry

Plasmid

#177183

Purpose

plasmid expressing an sgRNA targeting the PEAR-GFP plasmid along with an mCherry marker

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

sgRNA targeting the PEAR-GFP plasmid

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-EGFP-Bulged-miR-19

Plasmid

#91976

Purpose

EGFP reporter cell line with eight bulged miR-19 sites

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

EGFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLSU4mg10

Plasmid

#126079

Purpose

Plant transformation vector for stable expression of mitochondria targeted GFP1-10 in plant cells

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Binary

Tags

GFP1-10

Expression

Plant

Mutation

Promoter

'long' CaMV35S

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA FLAG mTOR ST

Plasmid

#22999

Depositor

Jing Huang

Article

Lomenick et al Proc Natl Acad Sci U S A. 2009 Dec 7. ()

Insert

mTOR (MTOR Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S2035T

Promoter

Available since

May 18, 2010

Availability

Academic Institutions and Nonprofits only
pCMV-M2-TLR2-P681H

Plasmid

#12290

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Use

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

A74V, M82I, H202Y and P681H; signal peptide (aa1-…

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156)

Plasmid

#61939

Purpose

Co-expression of hUCH37 (ISF1) and hNFRKB (1-156) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Inserts

hUCH37 (ISF1) (UCHL5 Human)
hNFRKB (1-156)

Use

Tags

6x HIS

Expression

Bacterial

Mutation

Construct ends at residue 156

Promoter

T7

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pCD5-D/bovine/Nebraska/9-5/2012-HEFwtED-GCN4-sfGFP-ST

Plasmid

#175019

Purpose

Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

Depositor

Robert de Vries

Article

Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

Insert

D/bovine/Nebraska/9-5/2012

Use

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCD5-ss-D/bovine/France/5920/2014-HEFwtED-GCN4-sfGFP-ST

Plasmid

#175017

Purpose

Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

Depositor

Robert de Vries

Article

Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

Insert

OK-HEFwtED

Use

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pJAG98-mTurquoise2-FHA2-AURKB substrate-YPet

Plasmid

#83286

Purpose

untargeted Aurora B FRET sensor with mTurquoise2 and YPet FRET pair

Depositor

Daniel Needleman

Article

Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

Insert

mTurquoise2-FHA2-AURKB substrate-YPet

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ_NAND2_L17_S19_S11

Plasmid

#132730

Purpose

T7 RNAP-driven expression of a 2-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 19 and 11

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_NAND2_L17_S19_S11

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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RBMS3_2xRRM_pGEX

Plasmid

#135142

Purpose

Recombinant expression of RBMS3 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBMS3 (RBMS3 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 10, 2021

Availability

Academic Institutions and Nonprofits only

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RBM24_RRM_pGEX

Plasmid

#135148

Purpose

Recombinant expression of RBM24 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBM24 (RBM24 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDGE673

Plasmid

#153239

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

GFP

Expression

Plant

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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GTH-c

Plasmid

#81103

Purpose

Gene Tagging vector HIS/CFP - Plasmid for yeast enhanced monomeric CFP (yemCFP) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

yemCFP

Use

Cre/Lox

Tags

yemCFP

Expression

Yeast

Mutation

Promoter

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCaf40

Plasmid

#79251

Purpose

Expresses EGFP-tagged DmCaf40 in S2 cells

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCaf40 (Rcd-1 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Expression

Mutation

Promoter

Ac5 promoter

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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GTL-y

Plasmid

#81100

Purpose

Gene Tagging vector LEU/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

mCitrine

Use

Cre/Lox

Tags

mCitrine

Expression

Yeast

Mutation

Mutated mCitrine for monomeric isoform (A206K L22…

Promoter

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pLaNGo11-3

Plasmid

#126054

Purpose

A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

chloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11x3 (5 aa linker)

Use

Binary

Tags

GFP1-10

Expression

Plant

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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