-
Plasmid#194671PurposeProtein production of the split HaloTag based calcium recorder Caprola_10 in bacteriaDepositorInsertCaprola_10
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_11
Plasmid#194672PurposeProtein production of the split HaloTag based calcium recorder Caprola_11 in bacteriaDepositorInsertCaprola_11
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_12
Plasmid#194673PurposeProtein production of the split HaloTag based calcium recorder Caprola_12 in bacteriaDepositorInsertCaprola_12
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_13
Plasmid#194674PurposeProtein production of the split HaloTag based calcium recorder Caprola_13 in bacteriaDepositorInsertCaprola_13
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_02
Plasmid#194663PurposeProtein production of the split HaloTag based calcium recorder Caprola_02 in bacteriaDepositorInsertCaprola_02
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_03
Plasmid#194664PurposeProtein production of the split HaloTag based calcium recorder Caprola_03 in bacteriaDepositorInsertCaprola_03
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Caspase-11_humanized-mutant
Plasmid#214315PurposeProtein overexpression in bacteriaDepositorInsertCASP4 (Casp4 Mouse)
UseTagsHis-TEVExpressionBacterialMutationN152K_KLS288YKT_KASIHS348TPRAKAPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Caspase-11_rat_catalytic domain
Plasmid#214329PurposeProtein overexpression in bacteriaDepositorInsertCASP4 (Casp4 Rat)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_DeltaN24
Plasmid#214327PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationDeletion of first 24 amino acidsPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_LES33AAA
Plasmid#214326PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationLES33AAAPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_DDEN29ENGD
Plasmid#214325PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationDDEN29ENGDPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_V47N_I48N
Plasmid#214324PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationV47N_I48NPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Caspase-5-enzymatic-domain
Plasmid#214306PurposeProtein overexpression in bacteriaDepositorInsertCASP5 (CASP5 Human)
UseTagsHis-TEVExpressionBacterialMutationaa133-434PromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Caspase-4_lemur_catalytic domain
Plasmid#214328PurposeProtein overexpression in bacteriaDepositorInsertCASP4
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Caspase-4_rabbit_catalytic domain
Plasmid#214330PurposeProtein overexpression in bacteriaDepositorInsertCASP4
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28b-TEV-AcrIC9
Plasmid#207409PurposeProtein purification vector to produce AcrIC9 in E. coliDepositorInsertT7-TEV-AcrIC9
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET23a-H6-TEV-AncSZ
Plasmid#214235PurposeBacterial expression construct of AncSZ, an kinase domain engineered through ancestorial reconstruction of the kinase domains of both SYK and ZAP-70. For in vitro protein tyrosine phosphorylationDepositorInsertSyk kinase (SYK Human)
UseTags6xHisExpressionBacterialMutationSequence gained through ancestral reconstruction …PromoterT7Available sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET23a-H6-TEV-cAbl
Plasmid#214234PurposeBacterial expression of the kinase domain of cAbl with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screeningsDepositorInsertABL1 Kinase domain (ABL1 Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET23a-H6-TEV-cSrc
Plasmid#214233PurposeBacterial expression of the kinase domain of cSrc with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screeningsDepositorInsertcSrc Kinase domain (SRC Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7REwt
Plasmid#213935PurposeGST tagged AO7 encoding aa 126-258 for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
UseTagsGSTExpressionBacterialMutationAO7 cloned into the first multiple cloning site o…PromoterT7 promoter-1Available sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only