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Plasmid#186460PurposeExpresses human alpha-catenin (residues 22-906) with an N-terminal His tag and a PRescission Protease cleavage siteDepositorInsertCTNNA1 (CTNNA1 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET16b-MYPT1(1-314)
Plasmid#224893PurposeBacterial expression of MYPT1(1-314)DepositorInsertmyosin phosphatase targeting subunit 1 (PPP1R12A )
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-CsHPAD_beta-gamma
Plasmid#226981PurposeExpressing Clostridium scatologenes hydroxyphenylacetate decarboxylase (HPAD) beta/gamma subunits in E. coliDepositorInsertCsHPAD_beta-gamma
UseTagsHis6 (on backbone)ExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-OuIAD(W392A)
Plasmid#226980PurposeExpressing Olsenella uli indoleacetate decarboxylase (IAD) W392A variant in E. coliDepositorInsertOuIAD(W392A)
UseTagsHis6 (on backbone)ExpressionBacterialMutationW392APromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-OuIAD(W392F)
Plasmid#226979PurposeExpressing Olsenella uli indoleacetate decarboxylase (IAD) W392F variant in E. coliDepositorInsertOuIAD(W392F)
UseTagsHis6 (on backbone)ExpressionBacterialMutationW392FPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-OuIAD(F401A)
Plasmid#226978PurposeExpressing Olsenella uli indoleacetate decarboxylase (IAD) F401A variant in E. coliDepositorInsertOuIAD(F401A)
UseTagsHis6 (on backbone)ExpressionBacterialMutationF401APromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-OuIAD(L616E)
Plasmid#226977PurposeExpressing Olsenella uli indoleacetate decarboxylase (IAD) L616E variant in E. coliDepositorInsertOuIAD(L616E)
UseTagsHis6 (on backbone)ExpressionBacterialMutationL616EPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-OuIAD(R226K)
Plasmid#226976PurposeExpressing Olsenella uli indoleacetate decarboxylase (IAD) R226K variant in E. coliDepositorInsertOuIAD(R226K)
UseTagsHis6 (on backbone)ExpressionBacterialMutationR226KPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-OuIAD(R226M)
Plasmid#226975PurposeExpressing Olsenella uli indoleacetate decarboxylase (IAD) R226M variant in E. coliDepositorInsertOuIAD(R226M)
UseTagsHis6 (on backbone)ExpressionBacterialMutationR226MPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-OuIAD(R226E)
Plasmid#226974PurposeExpressing Olsenella uli indoleacetate decarboxylase (IAD) R226E variant in E. coliDepositorInsertOuIAD(R226E)
UseTagsHis6 (on backbone)ExpressionBacterialMutationR226EPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-OuIAD(H514E)
Plasmid#226972PurposeExpressing Olsenella uli indoleacetate decarboxylase (IAD) H514E variant in E. coliDepositorInsertOuIAD(H514E)
UseTagsHis6 (on backbone)ExpressionBacterialMutationH514EPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-OuIAD(H514A)
Plasmid#226971PurposeExpressing Olsenella uli indoleacetate decarboxylase (IAD) H514A variant in E. coliDepositorInsertOuIAD(H514A)
UseTagsHis6 (on backbone)ExpressionBacterialMutationH514APromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-OuIAD(C500S)
Plasmid#226970PurposeExpressing Olsenella uli indoleacetate decarboxylase (IAD) C500S variant in E. coliDepositorInsertOuIAD(C500S)
UseTagsHis6 (on backbone)ExpressionBacterialMutationC500SPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-OuIAD(G853A)
Plasmid#226969PurposeExpressing Olsenella uli indoleacetate decarboxylase (IAD) G853A variant in E. coliDepositorInsertOuIAD(G853A)
UseTagsHis6 (on backbone)ExpressionBacterialMutationG853APromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_W4-HECT
Plasmid#187710PurposeExpress his-tagged NEDD4L W4-HECT in bacterial cellsDepositorInsertNEDD4L residues 543-975 (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-542PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_W3-W4-HECT_C874only
Plasmid#187711PurposeExpress his-tagged NEDD4L W3-W4-HECT_C874only in bacterial cellsDepositorInsertNEDD4L residues 492-975 with mutations (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-491; mutations C702A, C776…PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanIV-Streptomyces
Plasmid#226250PurposeExpresses precursor peptide and lanthipeptide synthetase from Streptomyces LanIV (FAST-RiPPs) in E. coliDepositorInsertLanA (LanIV-Streptomyces)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-a(+)-6xHis-LSSmCherry1(-13)
Plasmid#219415PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorArticleInsertLSSmCherry1(-13)
UseTagsMGHHHHHGGExpressionBacterialMutationK137E, K199EPromoterT7Available sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-a(+)-6xHis-mScarlet-I(+5)
Plasmid#219418PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorArticleInsertmScarlet-I(+5)
UseTagsMGHHHHHGGExpressionBacterialMutationD125K, D186K, E216K, E238KPromoterT7Available sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET29b(+)_gp1(A11V)
Plasmid#225131PurposePhage GIL01 protein gp1 (A11V) mutant with a single nucleotide substitution (C32T)DepositorInsertgp1 (A11V)
UseTagsHis-tagExpressionBacterialMutationAlanine 11 to ValinePromoterT7 promoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only