Addgene

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Showing: 2181 - 2200 of 24597 results

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RcSxph

Plasmid

#194018

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Use

Tags

His10 and mEGFP,

Expression

Mutation

Promoter

Polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pNK928

Plasmid

#219736

Purpose

MoClo-compatible Level 0 - promoter + 5U vector carrying promoter del5_MtHP

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

promoter of MtHP gene from Medicago truncatula, del5_MtHP

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

del5_MtHP

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_DelCTerm_1-223AA

Plasmid

#221476

Purpose

Gateway cloning of NKX2-1 C-terminal truncation mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Deletion of 224-371AA C-terminal domain

Promoter

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_DelNTerm_160-371AA

Plasmid

#221475

Purpose

Gateway cloning of NKX2-1 N-terminal truncation mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Deletion of 1-159AA N-terminal domain

Promoter

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_HD_160-223AA

Plasmid

#221477

Purpose

Gateway cloning of NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Deletion of 1-159AA N-terminal and 224-371AA C-te…

Promoter

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_DelNTerm_160-371AA

Plasmid

#221487

Purpose

NKX2-1 N-terminal truncation mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Deletion of 1-159AA N-terminal domain

Promoter

EF1a

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_DelCTerm_1-223AA

Plasmid

#221488

Purpose

NKX2-1 C-terminal truncation mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Deletion of 224-371AA C-terminal domain

Promoter

EF1a

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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TtA_pET29b

Plasmid

#215432

Purpose

Expression construct for TtA gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

TtA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS

Plasmid

#217478

Purpose

Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1s

Depositor

Peng Zou

Article

Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

Insert

Nier1s

Use

Tags

3xNLS, P2A-EGFP-3xNLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Nier1b-3NLS-P2A-EGFP-3NLS

Plasmid

#217479

Purpose

Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1b

Depositor

Peng Zou

Article

Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

Insert

Nier1b

Use

Tags

3xNLS, P2A-EGFP-3xNLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pKERep

Plasmid

#211826

Purpose

Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

Insert

eGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T5

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pKESa

Plasmid

#211828

Purpose

Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

Insert

eGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T5

Available since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo40

Plasmid

#204015

Purpose

Cloning plasmid containing the mCherry insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

mCherry fluorescent protein

Use

Tags

Expression

Plant

Mutation

Promoter

N/A

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo41

Plasmid

#204016

Purpose

Cloning plasmid containing the BFP insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Blue fluorescent protein

Use

Tags

Expression

Plant

Mutation

Promoter

N/A

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo42

Plasmid

#204017

Purpose

Cloning plasmid containing the YFP insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Yellow fluorescent protein

Use

Tags

Expression

Plant

Mutation

Promoter

N/A

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo6

Plasmid

#203957

Purpose

Cloning plasmid containing the kanamycin resistance gene insert

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Kanamycin Resistance Gene

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo7

Plasmid

#203958

Purpose

Cloning plasmid containing the ampicillin resistance gene insert

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Ampicillin Resistance Gene

Use

Tags

Expression

Plant

Mutation

Promoter

N/A

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo8

Plasmid

#203959

Purpose

Cloning plasmid containing the spectinomycin resistance gene insert

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Spectinomycin Resistance Gene

Use

Tags

Expression

Plant

Mutation

Promoter

N/A

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo12

Plasmid

#203982

Purpose

Cloning plasmid containing the rrnB T1 terminator insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

rrnB T1 Terminator

Use

Tags

Expression

Plant

Mutation

Promoter

N/A

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo3

Plasmid

#203921

Purpose

Cloning plasmid containing the ColE1 origin insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

ColE1 Origin of Replication

Use

Tags

Expression

Plant

Mutation

Promoter

N/A

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Showing: 2181 - 2200 of 24597 results

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