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Plasmid#163032PurposeFLiCRE protease for neuronal expressionDepositorInsertGFP-CaM-uTEVp
UseTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
His-Tev-UbE2D3
Plasmid#186805PurposeCodon-optimized bacterial expression plasmid. Expresses ubiquitin conjugating enzyme His-Tev-UbE2D3.DepositorInsertHis-Tev-UbE2D3 (UBE2D3 Human)
UseTagsHis6-TevExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-MBP-TEV
Plasmid#69929Purposebacterial expression of MBP-TEV n-terminal fusionsDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsMBP-TEVExpressionBacterialMutationPromoterT7Available sinceJan. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET22b-TEVP-cleavableELP
Plasmid#219402PurposeTobacco etch virus protease-responsive elastin-like polypeptide (ELP)DepositorInsertTEVP-cleavableELP
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
His6-MBP-uTEV3
Plasmid#135464PurposeBacterial expression of uTEV3 (full-length) proteaseDepositorInsertHis6-MBP-uTEV3
UseTagsMBP, His6ExpressionBacterialMutationS219V mutation (improves stability) , I138T/S153N…PromoterT5Available sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-MBP
Plasmid#105787PurposeExpression of your protein of interest in fusion with MBP at the C-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-MBPExpressionMammalianMutationPromoterAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-TO-ZscHCVP-TEVPZ
Plasmid#116094PurposeExpresses a combination of proteasesDepositorInsertZscHCVP-TEVPZ
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a hMDH1v3 (+TEV)
Plasmid#204229PurposeBacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).InserthMDH1v3 (MDH1 Human)
UseTags6X HisExpressionBacterialMutationInserted a G after ATG to maintain kozak sitePromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-FnCas12a-TEV
Plasmid#174658PurposeFnCas12a expression plasmidDepositorInsertFnCas12a
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-TEV-KRAS
Plasmid#111849PurposeExpresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS (KRAS Human)
UseTags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationPromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
SPELL-TEV Component 2
Plasmid#170490PurposeTEV (C-lobe) fused with FRBDepositorInsertmyc, FRB, TEV(C-lobe)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
SPELL-TEV Component 1
Plasmid#170489PurposeTEV (N-lobe) fused with iFKBPDepositorInsertTEV(N-lobe), iFKBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST-TEV-FUS
Plasmid#29629DepositorInsertFUS (FUS Human)
UseTagsGST and TEVExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
His-Tev-UbE1
Plasmid#186804PurposeCodon-optimized bacterial expression plasmid. Expresses ubiquitin activating enzyme His-Tev-UbE1.DepositorInsertHis-Tev-UbE1 (UBA1 Human)
UseTagsHis6-TevExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-Tev
Plasmid#177142PurposeBacterial vector for expression of an N-terminal GST fusion of the Tev proteaseDepositorInsertTev
UseTagsGSTExpressionBacterialMutationPromoterTacAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNIC-MBP-TEV-GG
Plasmid#215794PurposeCloning of target gene with N terminal MBP tag and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKK-TEV-CyOFP1
Plasmid#105799PurposeExpression of your protein of interest in fusion with orange fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-CyOFP1ExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pnCS_SEPT7_SEPT9_i1-TEV-Strep
Plasmid#174500Purposebacterial co-expression of human SEPT7 and of human SEPT9_i1DepositorUseTagsTEV-StrepExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-SBP-TEV
Plasmid#36943PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTags6xHistag-SBP-TEVExpressionBacterialMutationPromoterAvailable sinceJune 19, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pnCS_SEPT6_SEPT7-TEV-Strep
Plasmid#174499Purposebacterial co-expression of human SEPT6 and of human SEPT7DepositorUseTagsTEV-StrepExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only