Addgene

We narrowed to 1,772 results for: smo

Showing: 201 - 220 of 1772 results

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pGEX mSmo-Cterm

Plasmid

#58702

Purpose

used for anti-mouse Smoothened antibody production (for injection into animals)

Depositor

Matthew Scott

Article

Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

Insert

Smo (Smo Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

contains C terminus (aa 543-793)

Promoter

tac

Available since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only
SmoM2 (W535L)-pcw107

Plasmid

#64627

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

SMO (SMO Human)

Use

Lentiviral

Tags

Expression

Mutation

W535L

Promoter

PGK

Available since

May 15, 2015

Availability

Academic Institutions and Nonprofits only
pET28 mSmo-Cterm

Plasmid

#58703

Purpose

used for anti-mouse Smoothened antibody production (for affinity purification)

Depositor

Matthew Scott

Article

Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

Insert

Smo (Smo Mouse)

Use

Tags

6X-HIS

Expression

Bacterial

Mutation

contains C terminus (aa 543-793)

Promoter

Available since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only
1105-Desmoglein2-FLAG-eGFP

Plasmid

#36989

Depositor

Kathleen Green

Article

Klessner et al Mol Biol Cell. 2009 Jan;20(1):328-37. Ep

Insert

Dsg2 (DSG2 Human)

Use

Tags

FLAG and eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pLSSmOrange-mKate2 caspase-3 biosensor

Plasmid

#37132

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

Insert

LSSmOrange-DEVD-mKate2

Use

Tags

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoPSmOrange-Kan

Plasmid

#44951

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoconvertible mOrange with Kan selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPSmOrange

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoPSmOrange-SpHis5

Plasmid

#44856

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoconvertible mOrange with SpHis5 selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPSmOrange

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2 YFP-Smo-Weyer's

Plasmid

#41014

Depositor

Rajat Rohatgi

Article

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

smoothened homolog (Smo Mouse)

Use

Tags

EVC2 (aa 1177-1220) and YFP

Expression

Mammalian

Mutation

YFP inserted after Smo signal sequence. Transfer…

Promoter

Available since

Nov. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pCS2 YFP-Smo-Weyer'sAla5

Plasmid

#41016

Depositor

Rajat Rohatgi

Article

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

smoothened homolog (Smo Mouse)

Use

Tags

EVC2 ala5 (aa 1177-1220 with amino acids 1185-118…

Expression

Mammalian

Mutation

YFP inserted after Smo signal sequence. Transfer…

Promoter

Available since

Nov. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pEF5B-FRT-AP-Smo-YFP-DEST

Plasmid

#49099

Purpose

Expressing AP-Smo-YFP in mammalian cells, can be used for establishing Flp-In stable cell lines

Depositor

Maxence Nachury

Article

Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

Insert

Smo (Smo Mouse)

Use

Tags

YFP and acceptor peptide (AP)

Expression

Mammalian

Mutation

Promoter

T7

Available since

Nov. 8, 2013

Availability

Academic Institutions and Nonprofits only

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PD22

Plasmid

#164531

Purpose

Expresses Smo-FLAG

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

WT

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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GP736

Plasmid

#164532

Purpose

Express Smo-FLAG-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened (Smo Mouse)

Use

Lentiviral

Tags

FLAG-Ub

Expression

Mutation

WT

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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GP799

Plasmid

#164544

Purpose

Expresses Smo-FLAG

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

WT

Promoter

GgCryD1

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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GP777

Plasmid

#164533

Purpose

Expresses Smo noK-FLAG

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened noK (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

All 21 cytoplasmic Lysines of Smoothened changed …

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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GP821

Plasmid

#164552

Purpose

Expresses Smo-FLAG K434R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K434R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

One of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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GP823

Plasmid

#164554

Purpose

Expresses Smo-FLAG K448R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K448R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

One of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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GP822

Plasmid

#164553

Purpose

Expresses Smo-FLAG K444R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K444R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

One of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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GP806

Plasmid

#164534

Purpose

Expresses Smo noK-FLAG-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened noK (Smo Mouse)

Use

Lentiviral

Tags

FLAG-Ub

Expression

Mutation

All 21 cytoplasmic Lysines of Smoothened changed …

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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GP803

Plasmid

#164547

Purpose

Expresses Smo-FLAG K434, 444, 448R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K434, 444, 448R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

All three Lysines in cytoplasmic loop three of Sm…

Promoter

GgCryD1

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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GP802

Plasmid

#164546

Purpose

Expresses Smo-FLAG K348, 360R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K348, 360R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Both the Lysines in cytoplasmic loop two of Smoo…

Promoter

GgCryD1

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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