Addgene

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Showing: 201 - 220 of 63820 results

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pMax_mOrange_A215C

Plasmid

#177828

Purpose

Site-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

mOrange_A215C

Use

Tags

Expression

Mammalian

Mutation

A215C

Promoter

CMV

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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mOrange-N1

Plasmid

#54499

Purpose

Localization: N1 Cloning Vector, Excitation: 548, Emission: 562

Depositor

Michael Davidson

Article

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Type

Empty backbone

Use

Tags

mOrange

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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mOrange-C1

Plasmid

#54680

Purpose

Localization: C1 Cloning Vector, Excitation: 548, Emission: 562

Depositor

Michael Davidson

Article

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Type

Empty backbone

Use

Tags

mOrange

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pTRANS_220d

Plasmid

#91114

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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RANLS

Plasmid

#50843

Purpose

Expresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddRFP A

Use

Tags

triplicated NLS sequence DPKKKRKV

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pMax_mOrange_G299C

Plasmid

#177829

Purpose

Site-specific mutagenesis of mOrange for the purposes of creating a site-specific G:G mispair for the purposes of measuring MMR activity via Host Cell Reactivation (HCR).

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

mOrange_G299C

Use

Tags

Expression

Mammalian

Mutation

G299C

Promoter

CMV

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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mOrange2-N1

Plasmid

#54568

Purpose

Localization: N1 Cloning Vector, Excitation: 549, Emission: 565

Depositor

Roger Tsien, Nathan Shaner, Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Type

Empty backbone

Use

Tags

mOrange2

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pRN3P_membrane_EGFP

Plasmid

#139402

Purpose

in vitro transcription for mRNA

Depositor

Maria-Elena Torres-Padilla

Article

Borsos et al Nature. 2019 May;569(7758):729-733. doi:

Insert

membrane-EGFP

Use

In vitro transcription

Tags

EGFP

Expression

Mutation

Promoter

T3

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLSSmOrange-C1

Plasmid

#37131

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

Insert

LSSmOrange

Use

Tags

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pLSSmOrange-N1

Plasmid

#37130

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

Insert

LSSmOrange

Use

Tags

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pTRANS_221

Plasmid

#91115

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: BeYDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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ChromosomesMembranesandCytoskeleton

Plasmid

#62450

Purpose

MXS_chaining vector with 4 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

CMV::H2B-TagBFPx3-bGHpA
CMV::Lyn-Ceruleanx3-bGHpA
CMV::mCherryx3-linker-betaActin-bGHpA
CMV::Citrinex3-linker-alphaTubulin-bGHpA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCLASP_mScarlet_CLASP_RGS2membrane

Plasmid

#133086

Purpose

Plasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to bl

Depositor

Hana El-Samad

Article

Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

Insert

mScarlet-CLASP

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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mOrange2-C1

Plasmid

#54650

Purpose

Localization: C1 Cloning Vector, Excitation: 549, Emission: 565

Depositor

Roger Tsien, Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Type

Empty backbone

Use

Tags

mOrange2

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pTRANS_201

Plasmid

#91105

Purpose

Transformation, Type: T-DNA, Plant Selection: none, Viral Replication: BeYDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

No Promoter

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTRANS_210

Plasmid

#91108

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRANS_211

Plasmid

#91110

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: BeYDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pTRANS_260d

Plasmid

#91126

Purpose

Transformation, Type: T-DNA, Plant Selection: PvUbi2:bar, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

PvUbi2

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pPSmOrange-C1

Plasmid

#31899

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Use

Tags

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S (mutations…

Promoter

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pTRANS_150

Plasmid

#91205

Purpose

Transformation, Type: non-T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge