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Showing: 201 - 220 of 649 results

  1. pCas1_Cas2/3

    Plasmid
    #89240
    Purpose
    Plasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1
    Depositor
    Blake Wiedenheft
    Article
    Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p
    Insert
    Cas1 and Cas2/3
    Use
    Tags
    Strep II tag on Cas1
    Expression
    Mutation
    Promoter
    Available since
    May 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89240-plasmid-map-sequence-id-176381

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  4. pGP-AAV-syn-flex-iGABASnFR2-WPRE

    Plasmid
    #218877
    Purpose
    AAV-mediated expression of improved GABA sensor (positive change in fluorescence)
    Depositor
    GENIE Project
    Has Service
    AAV1 and AAV5
    Insert
    iGABASnFR2
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    S99A F102Y F104Y L178S
    Promoter
    Synapsin
    Available since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    218877-plasmid-map-sequence-id-436595

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  9. pGP-AAV-CAG-flex-iGABASnFR2(no bind)-WPRE

    Plasmid
    #218882
    Purpose
    AAV-mediated expression of improved GABA sensor control (no change in fluorescence)
    Depositor
    GENIE Project
    Insert
    iGABASnFR2(no bind)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    S99A F102G R168P
    Promoter
    CAG
    Available since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    218882-plasmid-map-sequence-id-436608

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  10. pGP-AAV-syn-flex-iGABASnFR2(no bind)-WPRE

    Plasmid
    #218879
    Purpose
    AAV-mediated expression of improved GABA sensor control (no change in fluorescence)
    Depositor
    GENIE Project
    Has Service
    AAV1 and AAV5
    Insert
    iGABASnFR2(no bind)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    S99A F102G R168P
    Promoter
    Synapsin
    Available since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    218879-plasmid-map-sequence-id-436597

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  18. 13SA-csy4

    Plasmid
    #97037
    Purpose
    Spectinomycin resistant plasmid with a CDF origin that expresses Csy4 when IPTG induced, for coexpression with Series 371 vectors
    Depositor
    Chris Jeans
    Insert
    Cys4
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97037-plasmid-map-sequence-id-182491

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  19. pCsy_complex

    Plasmid
    #89232
    Purpose
    Plasmid contains the genes Csy1, Csy2, Csy3, Csy4, cloned as a polycistronic operon from bacterium Pseudomonas aeruginosa PA14. Csy3 includes an N-terminal 6his tag and TEV protease cleavage site
    Depositor
    Blake Wiedenheft
    Article
    Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:
    Inserts
    Csy1
    Csy2
    Csy3
    Csy4
    Use
    Tags
    6xHis-TEV protease
    Expression
    Mutation
    Promoter
    Available since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    89232-plasmid-map-sequence-id-177172

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Showing: 201 - 220 of 649 results