Addgene

We narrowed to 567 results for: pBR322

Showing: 201 - 220 of 567 results

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pJH26_pBad_AraC_Para-sfGFP.1TAG

Plasmid

#163721

Purpose

Arabinose-inducible expression of green fluorescent protein encoding one amber codon

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

araC gfp.1TAG

Use

Tags

Expression

Bacterial

Mutation

gfp.N39TAG

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH27_pBad_AraC_Para-sfGFP.2TAG

Plasmid

#163722

Purpose

Arabinose-inducible expression of green fluorescent protein encoding two amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

araC gfp.2TAG

Use

Tags

Expression

Bacterial

Mutation

gfp.N39TAG,Y151TAG

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH807_pJH_LldR_Pcon-sfGFP.2TAG

Plasmid

#163738

Purpose

Constitutive expression of green fluorescent protein encoding two stop codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.2TAG

Use

Tags

Expression

Bacterial

Mutation

gfp.N39TAG,Y135TAG

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH809_pJH_LldR_Pcon-sfGFP.10TAG

Plasmid

#163739

Purpose

Constitutive expression of green fluorescent protein encoding ten stop codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.10TAG

Use

Tags

Expression

Bacterial

Mutation

gfp with first 10 leucine codons mutated to amber

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pRF032d

Plasmid

#156353

Purpose

Expresses heterologous rRNA

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

rRNA operon, orthogonal-antiRBS

Use

Synthetic Biology

Tags

Expression

Mutation

WT antiRBS engineered to orthogonal antiRBS, PS e…

Promoter

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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PJ14

Plasmid

#25912

Depositor

Kenneth Marcu

Article

Lang et al Nucleic Acids Res. 1982 Jan 22. 10(2):61

Insert

Ig germline mu switch region

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only
dSp-Rta 125-190

Plasmid

#99664

Purpose

Expresses dSp Cas9 fused to truncated RTA activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated RTA activation domain ( 125-190)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pDHJS3 AS-

Plasmid

#78237

Purpose

One of four plasmids needed to create the double Holliday junction substrate (DHJS).

Depositor

Tao-shih Hsieh

Article

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "B"
loxP2

Use

Phagemid

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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OgeuIscB ωRNA-v2

Plasmid

#220958

Purpose

human U6-driven expression of ωRNA-v2 scaffold with BsmBI for guide cloing

Depositor

Weixin Tang

Article

Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/

Insert

OgeuIscB-ωRNA-v2 scaffold with BsmBI for guide cloning

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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dSp-Rta 125-175

Plasmid

#99668

Purpose

Expresses dSp Cas9 fused to truncated RTA activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated RTA activation domain (125-175)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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dSp-VP64-RTA(75-190)

Plasmid

#99669

Purpose

Expresses dSp Cas9 fused to combination of truncated activation domains

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

VP64-RTA(75-190)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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dSp-p65 (1-100)

Plasmid

#99660

Purpose

Expresses dSp Cas9 fused to truncated p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated p65 activation domain (1-100)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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dSp-p65 (50-150)

Plasmid

#99661

Purpose

Expresses dSp Cas9 fused to truncated p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated p65 activation domain (50-150)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
dSp-Rta 75-190

Plasmid

#99663

Purpose

Expresses dSp Cas9 fused to truncated RTA activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated RTA activation domain (75-190)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
dSp-Rta 75-175

Plasmid

#99666

Purpose

Expresses dSp Cas9 fused to truncated RTA activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated RTA activation domain (75-175)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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dSp-p65 Full (1-261)

Plasmid

#99654

Purpose

Expresses dSp Cas9 fused to p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

p65 activation domain

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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dSp-p65 (150-261)

Plasmid

#99655

Purpose

Expresses dSp Cas9 fused to truncated p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated p65 activation domain (150-261)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
dSp-p65 (100-261)

Plasmid

#99656

Purpose

Expresses dSp Cas9 fused to truncated p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated p65 activation domain (100-261)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
dSp-p65 (200-261)

Plasmid

#99657

Purpose

Expresses dSp Cas9 fused to truncated p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated p65 activation domain (200-261)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
dSp-p65 (1-200)

Plasmid

#99658

Purpose

Expresses dSp Cas9 fused to truncated p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated p65 activation domain (1-200)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge