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Plasmid#112906PurposeMultisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mCitrine.DepositorTypeEmpty backboneUseOomycete expressionTagsExpressionMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only
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pRSETA_crG18
Plasmid#172930PurposeCrowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 18x GSGDepositorInsertCrowdingSensorG18
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FRT-FAK-kinase30
Plasmid#59124PurposeFAK fret control sensor without FERM domain with 30 nm linkerDepositorInsertFocal Adhesion Kinase
UseTagsFLAG, mCerulean, and mCitrineExpressionMammalianMutationPromoterCMVAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTORKm43GWY
Plasmid#112901PurposeMultisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mCitrine.DepositorTypeEmpty backboneUseOomycete expressionTagsExpressionMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FRT-FAK-kinase0
Plasmid#59122PurposeFAK fret control sensor without FERM domain with no linkerDepositorInsertFocal Adhesion Kinase
UseTagsFLAG, mCerulean, and mCitrineExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FRT-FAK-FERM0
Plasmid#59125PurposeFAK fret control sensor without kinase domain with no linkerDepositorInsertFocal Adhesion Kinase
UseTagsFLAG, mCerulean, and mCitrineExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FRT-FAK-kinase10
Plasmid#59123PurposeFAK fret control sensor without FERM domain with 10 nm linkerDepositorInsertFocal Adhesion Kinase
UseTagsFLAG, mCerulean, and mCitrineExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FRT-FAK-FERM10
Plasmid#59126PurposeFAK fret control sensor without kinase domain with 10 nm linkerDepositorInsertFocal Adhesion Kinase
UseTagsFLAG, mCerulean, and mCitrineExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCit-4aa-SNAP25B
Plasmid#53235PurposeFluorescent probe: SNAP-25B, N-terminally-tagged with monomeric Citrine, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCer-4aa-VAMP2 to measure FRET and dispersion.DepositorInsertSNAP-25B (Snap25 Rat)
UseTags4aa-linker and mCitrineExpressionMammalianMutationPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
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aCKAR
Plasmid#124427PurposeFRET-based reporter for monitoring aPKC activity in cellsDepositorInsertaCKAR
UseTagsmCFP and mCitrineExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
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JD179
Plasmid#229496PurposeAdenoviral helper plasmid containing inducible E2A and L4-22k/33k genes and constitutive VA RNA I geneDepositorInsertE2A, L4-22k/33k, VA RNA I
UseAAV and AdenoviralTagsExpressionMammalianMutationPromoterTre (E2A, L4-22k/33k). Wt promoter (VA RNA I), ET…Available sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCit-APPAP-gs
Plasmid#228090PurposeMammalian expression of five coiled coil segments in APPAP arrangment, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.DepositorInsertmCit, APPAP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCit-APPAPAPPAP-gs
Plasmid#228091PurposeMammalian expression of 10 coiled coil segments in APPAPAPPAP arrangement, with short flexible linkers and N-terminal mCitrine for phase separation and forming of liquid condensates in mammalian cellsDepositorInsertmCit, APPAPAPPAP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
VAMP2-12aa-mCit
Plasmid#53237PurposeFluorescent probe: SNAP-25B, C-terminally-tagged with monomeric Citrine, connected with a 12 amino acid linker. Expressed in mammalian cells, used with Stx1a-12aa-mCer to measure FRET and dispersion.DepositorInsertVAMP2 (Vamp2 Rat)
UseTags12aa-linker and mCitrineExpressionMammalianMutationPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
RFP-PPPPPPAAAA-gs
Plasmid#228092PurposeMammalian expression of 10 coiled coil segments in PPPPPPAAAA arrangement, with short flexible linkers and N-terminal mCitrine for phase separation and forming of liquid condensates in mammalian cellsDepositorInsertmCit, PPPPPPAAAA
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCit(S1h)8-gs
Plasmid#228084PurposeMammalian expression of coiled coil segments (S1h)x8, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.DepositorInsertmCit, S1h
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCit(P5f-P13f)3-gs
Plasmid#228080PurposeMammalian expression of coiled coil segments (P5f-P13f)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.DepositorInsertmCit, P5f, P13f
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCit(S1h)3(S3h)3-gs
Plasmid#228079PurposeMammalian expression of coiled coil segments (S1h)x3-(S3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.DepositorInsertmCit, S1h, S3h
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only