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Plasmid#147924PurposeMammalian Expression of 6xbsMS2-MalatDepositorInsert6xbsMS2-Malat (Malat1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pORANGE LOX Cloning template vector
Plasmid#139651PurposeCloning template for ORANGE-CAKE conditional knock-in constructsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterU6Available sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-K27 histone methylation reporter
Plasmid#22865DepositorInsertK27 HMR
UseTagsCFP and YFPExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBx1-VHH template1-3XMyc-Spacer
Plasmid#176208PurposePCR template for generating CeVICA VHH/nanobody libraryDepositorInsertVHH template1-3XMyc-Spacer
UseTagsMyc tagExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBx1-VHH template3-3XMyc-Spacer
Plasmid#176210PurposePCR template for generating CeVICA VHH/nanobody libraryDepositorInsertVHH template3-3XMyc-Spacer
UseTagsMyc tagExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBx1-VHH template2-3XMyc-Spacer
Plasmid#176209PurposePCR template for generating CeVICA VHH/nanobody libraryDepositorInsertVHH template2-3XMyc-Spacer
UseTagsMyc tagExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
p2xFlag CMV2 Lats1-PY1+2 mutant
Plasmid#18975DepositorInsertLarge tumor suppressor kinase 1 PY1+2 mutant (LATS1 Human)
UseTags2xFlagExpressionMammalianMutationPY1 mutant, (Tyrosine 376 changed to Alanine) and…PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
p2xFlag CMV2 Lats1-PY1 mutant
Plasmid#18973DepositorInsertLarge tumor suppressor kinase 1 PY-1 mutant (LATS1 Human)
UseTags2xFlagExpressionMammalianMutationPY1 mutant, Tyrosine 376 changed to AlaninePromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRSETB-S28 histone 3 phosphorylation reporter
Plasmid#32788DepositorInsertCFP-14-3-3tau-H3 peptide-YFP
UseTagsCFP and YFPExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
p2xFlag CMV2 Lats1-PY2 mutant
Plasmid#18974DepositorInsertNM_004690 (LATS1 Human)
UseTags2xFlagExpressionMammalianMutationPY2 mutant (Tyrosine 559 changed to Alanine)PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
human SLC12A5-V5-HA-2A-Luciferase repair template
Plasmid#158576PurposeFor targeted insertion of V5-HA-2A-Luciferase reporter before the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.DepositorInserthuman SLC12A5 5' homology arm-V5-HA-2A-Luciferase-3' homology arm (SLC12A5 Human)
UseTagsV5 and HA on the endogenous KCC2 protein productExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
AtS/MAR10 insulator sequence (GB3458)
Plasmid#160638PurposepEGB3a1 AtS/MAR10DepositorInsertAtS/MAR10 insulator sequence
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Fibronectin-human-mutated V1 O-Glycosylation site
Plasmid#120406Purposeenables eukaryotic expression of human plasma fibronectin in which the third O-glycosylation site was mutated from threonine to serineDepositorInsertFibronectin (FN1 Human)
UseTagsExpressionMammalianMutationContains complete variable region with a mutation…PromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-Halo-PILATeS-RGD (200nM)
Plasmid#216716PurposeExpresses HaloTag-PILATeS-RGD with 200 nM BiT tag in bacteriaDepositorInsertHaloTag-PILATeS-RGD
UseTags6xHis and HaloTagExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCh-LgBiT-2A-EGFP-Vin-PILATeS
Plasmid#216761PurposePiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS (700 pM) in mammalian cells. Requires transposaseDepositorInsertmCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT
Plasmid#216763PurposePiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposaseDepositorInsertmCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-Halo-PILATeS-RGD (700pM)
Plasmid#216714PurposeExpresses HaloTag-PILATeS-RGD with 700 pM BiT tag in bacteriaDepositorInsertHaloTag-PILATeS-RGD
UseTags6xHis and HaloTagExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-PlatTET-wpA-pPuroTK
Plasmid#214125PurposeGene expression by Piggybac transposase in mammalian cellsDepositorInsertdCas9-TET
UseTagsExpressionMammalianMutationCodon-optimized for mammalian cellsPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-luc-TNFalpha-ARE-polyA-Malat_AH
Plasmid#148878PurposeMammalian Expression of TNFalpha-ARE-polyA-MalatDepositorInsertTNFalpha-ARE-polyA-Malat (TNF Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-Rluc-6xbsMS2-polyA-Malat_AC
Plasmid#148430PurposeMammalian Expression of 6xbsMS2-polyA-MalatDepositorInsert6xbsMS2-polyA-Malat
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only