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Showing: 201 - 220 of 4895 results

  1. pFH93

    Plasmid
    #128196
    Purpose
    Level1 ZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOSt
    Depositor
    Vladimir Nekrasov
    Article
    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d
    Insert
    ZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOSt
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    128196-plasmid-map-sequence-id-252933

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  4. pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003)

    Plasmid
    #174138
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRER=D1135V/G1218R/R1335E/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174138-plasmid-map-sequence-id-341915

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  5. pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)

    Plasmid
    #174139
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRQR=D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174139-plasmid-map-sequence-id-341913

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  12. pFH70

    Plasmid
    #128190
    Purpose
    Level1 ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt
    Depositor
    Vladimir Nekrasov
    Article
    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d
    Insert
    ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    128190-plasmid-map-sequence-id-252944

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  15. pTDpelB-C_sfYFPTwinStrep

    Plasmid
    #45944
    Purpose
    expresses P.putida codon optimized PelB-sfYFP-Twin-Strep fusion protein for periplasmic translocation via PelB signal sequence
    Depositor
    Thorben Dammeyer
    Article
    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.
    Insert
    synthetic sfYFP (codon usage adapted to P.putida KT2440)
    Use
    Tags
    PelB export sequence and Twin-Strep-tag
    Expression
    Bacterial
    Mutation
    Promoter
    lacIq/Ptrc
    Available since
    Sept. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    45944-plasmid-map-sequence-id-64657

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  16. pUB_SM-FLuc100_EMCV-Ren

    Plasmid
    #210591
    Purpose
    Expresses SM(FLAG)-optimal Firefly luciferase, and EMCVIRES-driven Renilla luciferase
    Depositor
    Olivia Rissland
    Article
    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi
    Inserts
    Spaghetti monster (FLAG)-optimal FLuc
    Optimal Renilla luciferase
    Use
    Luciferase
    Tags
    Spaghetti monster (FLAG)
    Expression
    Mammalian
    Mutation
    Promoter
    EMCV IRES and UBC human
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    210591-plasmid-map-sequence-id-416807

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  18. pcDNA3.1(-)-V5.mER.hOr47a

    Plasmid
    #126473
    Purpose
    Human codon-optimized version of D. melanogaster Or47a (hOr47a) with an N-terminal tag derived from human HCN1 (mER) for improved intracellular trafficking in mammalian cells
    Depositor
    Bill Hansson
    Article
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Insert
    Or47a (Or47a Fly)
    Use
    Tags
    H. sapiens HCN1 106VNKFSL111 (mER) and V5 tag
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens
    Promoter
    CMV
    Available since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    126473-plasmid-map-sequence-id-246528

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  19. R777-E237 Hs.RASAL3

    Plasmid
    #70521
    Purpose
    Gateway ORF clone of codon optimized human RASAL3 [NM_022904.1] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASAL3 (RASAL3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70521-plasmid-map-sequence-id-125970

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  20. R777-E309 Hs.SHC2

    Plasmid
    #70593
    Purpose
    Gateway ORF clone of codon optimized human SHC2 [NM_012435.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SHC2 (SHC2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70593-plasmid-map-sequence-id-126042

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Showing: 201 - 220 of 4895 results