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We narrowed to 220 results for: PP7

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SRAcode_in_pUC57_v3(419-999)

Plasmid

#49824

Purpose

for transcribing SRA nucl 419-999 of NM_001035235 with T7

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator RNA coding (SRA1 Human)

Use

Tags

Expression

Bacterial

Mutation

KpnI site at 5' start of RNA coding seq

Promoter

T7

Available since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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SRAcode_in_pUC57_v3(767-999)

Plasmid

#49825

Purpose

for transcribing SRA nucl 767-999 of NM_001035235 with T7

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator RNA coding (SRA1 Human)

Use

Tags

Expression

Bacterial

Mutation

KpnI site at 5' start of RNA coding seq

Promoter

T7

Available since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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SRAcode_in_pUC57_v3(419-771)

Plasmid

#49823

Purpose

for transcribing SRA nucl 419-771 of NM_001035235 with T7

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator RNA coding (SRA1 Human)

Use

Tags

Expression

Bacterial

Mutation

KpnI site at 5' start of RNA coding seq

Promoter

T7

Available since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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RAB11FIP5 gRNA (BRDN0001147136)

Plasmid

#76186

Purpose

3rd generation lentiviral gRNA plasmid targeting human RAB11FIP5

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RAB11FIP5 (RAB11FIP5 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RAB11FIP5 gRNA (BRDN0001148210)

Plasmid

#76185

Purpose

3rd generation lentiviral gRNA plasmid targeting human RAB11FIP5

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RAB11FIP5 (RAB11FIP5 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RAB11FIP5 gRNA (BRDN0001148953)

Plasmid

#76187

Purpose

3rd generation lentiviral gRNA plasmid targeting human RAB11FIP5

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RAB11FIP5 (RAB11FIP5 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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hSRAP.HMG6.wt.Dlink.a1-236

Plasmid

#49813

Purpose

expression vector for MBP-SRAP(1-236) fusion

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein (SRA1 Human)

Use

Tags

E. coli Maltose Binding Protein

Expression

Bacterial

Mutation

wildtype codon-optimized for E. coli expression

Promoter

T7

Available since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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hSRAP.TWIN1.a13-215

Plasmid

#49817

Purpose

expression vector for SRAP(13-215) in pTWIN1 vector

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein aa 13-215 (SRA1 Human)

Use

Tags

intein & chitin binding domain

Expression

Bacterial

Mutation

wildtype codon-optimized for E. coli expression

Promoter

T7

Available since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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hSRAP.HMG6.wt.Dlnk.a13-236

Plasmid

#49814

Purpose

expression vector for MBP-SRAP(13-236) fusion

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein (SRA1 Human)

Use

Tags

E. coli Maltose Binding Protein

Expression

Bacterial

Mutation

wildtype codon-optimized for E. coli expression

Promoter

T7

Available since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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hSRAP.TWIN1.a111-215

Plasmid

#49820

Purpose

expression vector for SRAP(111-215) in pTWIN1 vector

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein aa 111-215 (SRA1 Human)

Use

Tags

intein & chitin binding domain

Expression

Bacterial

Mutation

wildtype codon-optimized for E. coli expression

Promoter

T7

Available since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pmRFP-N1 human cofilin R21Q

Plasmid

#51279

Purpose

expresses human cofilin R21Q mutant fused to mRFP in mammalian cells

Depositor

James Bamburg

Article

Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:

Insert

cofilin 1 (CFL1 Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

R21Q, non-rod-forming mutation that can be used t…

Promoter

CMV

Available since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only
pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA

Plasmid

#105000

Purpose

Expresses ST-Renilla-Rh1 calibration reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

Depositor

Jeffrey Chao

Article

Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

Insert

Renilla luciferase

Use

Tags

24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …

Expression

Bacterial and Mammalian

Mutation

Promoter

TetCMV

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC38A5

Plasmid

#132118

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC38A5 (SLC38A5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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hSRAP.TWIN1.a105-236.V105M

Plasmid

#49819

Purpose

expression vector for SRAP(105-236)V105M in pTWIN1 vector

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein aa 105-236 (SRA1 Human)

Use

Tags

intein & chitin binding domain

Expression

Bacterial

Mutation

V105M codon-optimized for E. coli expression

Promoter

T7

Available since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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hSRAP.TWIN1.a105-215.V105M

Plasmid

#49818

Purpose

expression vector for SRAP(105-215)V105M in pTWIN1 vector

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein aa 105-215 (SRA1 Human)

Use

Tags

intein & chitin binding domain

Expression

Bacterial

Mutation

V105M codon-optimized for E. coli expression

Promoter

T7

Available since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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TFORF2955

Plasmid

#144431

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

HMG20B (HMG20B Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC38A5_STOP

Plasmid

#161284

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC38A5 (SLC38A5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pBiT3.1-N_CHAF1B:1-559

Plasmid

#211144

Purpose

Mammalian expression of full-length human CHAF1B. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CHAF1B:M1-P559 (CHAF1B Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_CHAF1B:1-559

Plasmid

#211185

Purpose

Mammalian expression of full-length human CHAF1B. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CHAF1B:M1-P559 (CHAF1B Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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CCSB-Broad Human Kinase ORF Collection

Plasmid Kit

#1000000014

Purpose

556 distinct human kinases and kinase-related protein ORFs in a pDONR-223 Entry Gateway vector. All clones are clonal isolates.

Depositor

William Hahn, David Root

Available since

July 16, 2011

Availability

Academic Institutions and Nonprofits only