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Showing: 201 - 220 of 286 results

  4. hU6-DR_BsmBI_DR-EFS-RfxCas13d-NLS-2A-Puro-WPRE CasRx pre-gRNA backbone

    Plasmid
    #219823
    Purpose
    EF1α-driven expression of RfxCas13d in mammalian cells. For cloning of pre-guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. (Adapted from plasmid #138147)
    Depositor
    Philipp Maass
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core promoter
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219823-plasmid-map-sequence-id-441127

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  14. SEPLuc

    Plasmid
    #141102
    Purpose
    bioluminescent reporter consisting of a membrane-bound SEP and Nanoluc fusion; for monitoring extracellular tumor acidosis
    Depositor
    Jeak Ling Ding
    Article
    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41
    Insert
    SEP-Nanoluc
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1α
    Available since
    May 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    141102-plasmid-map-sequence-id-296870

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  17. pDual-eGFP

    Plasmid
    #63215
    Purpose
    Expression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV-EF1α hybrid (CEF)
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. pDBEcR/Bla

    Plasmid
    #201556
    Purpose
    Plasmid with SB ITRs for expressing DBEcR (3xmyc-tagged) under EF1α promoter; blasticidin resistance.
    Depositor
    Randy Poon
    Article
    Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754
    Insert
    DBEcR
    Use
    Unspecified; Sleeping beauty transposon
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    201556-plasmid-map-sequence-id-400918

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Showing: 201 - 220 of 286 results