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Plasmid#118856Purposefluorescent protein expression with an N-terminal His-tagDepositorInsertEBFP2-LSSmOrange
UseTagsHis-tagExpressionBacterialMutationEBFP1.2-I128V/V150I/ D155V/V224R and for LSSmOra…PromoterAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBFP (GB0025)
Plasmid#68193PurposeProvides the CDS of the Blue fluorescente protein as a level 0 GoldenBraid partDepositorInsertBFP coding region
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G4_Q118R_Std_BFP-to-EGFP_Dual_epegRNA_tevopreQ1
Plasmid#187459PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 pegRNA and EBFP_To_EGFP epegRNA (tevopreq1) from two independent U6 promoters.DepositorInsertATP1A1 G4 Q118R pegRNA + EBFP to EGFP epegRNA (tevopreq1) (ATP1A1 Human)
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
FpG5.BFP.Reporter
Plasmid#134967PurposeLentiviral vector for enhancer reporter assayDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromotermini Fgf4 promoterAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-MyosinIIA-C-18
Plasmid#55315PurposeLocalization: Cytoskeleton, Excitation: 399, Emission: 456DepositorInsertMyosinIIA
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mTagBFP2-MAPTau-C-10
Plasmid#55311PurposeLocalization: Microtubules, Excitation: 399, Emission: 456DepositorInsertMAPTau (MAPT Human)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
EBFP2-Fibrillarin-7
Plasmid#55241PurposeLocalization: Nucleoli, Excitation: 383, Emission: 448DepositorInsertFibrillarin (FBL Human)
UseTagsEBFP2ExpressionMammalianMutationR36Q, S66FPromoterCMVAvailable sinceOct. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTS2337_Tier3(SB)-TagBFP2_Puro
Plasmid#169635PurposeTier-3 vector for stable integration of up to three cassettes via sleeping beauty transposase including a TagBFP marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mTagBFP2-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven mTagBFP2 and PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-Dectin1A-C-10
Plasmid#55290PurposeLocalization: Membrane, Excitation: 399, Emission: 456DepositorInsertDectin1A (CLEC7A Human)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mTagBFP2-Caveolin-C-10
Plasmid#55279PurposeLocalization: Membrane, Excitation: 399, Emission: 456DepositorInsertCaveolin (CAV1 Human)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mTagBFP2-CAF1-C-10
Plasmid#55277PurposeLocalization: Chromatin Assembly Factor 1, Excitation: 399, Emission: 456DepositorInsertCAF1
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mTagBFP2-MAPTau-N-10
Plasmid#55312PurposeLocalization: Microtubules, Excitation: 399, Emission: 456DepositorInsertMAPTau (MAPT Human)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mTagBFP2-Vinculin-23
Plasmid#55334PurposeLocalization: Focal Adhesions, Excitation: 399, Emission: 456DepositorInsertVinculin (VCL Human)
UseTagsmTagBFP2ExpressionMammalianMutationV234L in Vinculin.PromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pME-TagBFP-NS
Plasmid#75342PurposeMultisite gateway entry clone for adding N-terminal fusions of TagBFPDepositorInsertTagBFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-Nucleus-7
Plasmid#55249PurposeLocalization: Nuclear Localization, Excitation: 383, Emission: 448DepositorInsertSV40 T-Antigen Nuclear Localization Signal (NLS)
UseTagsEBFP2-NLSExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mTagBFP2-MCAK-C-7
Plasmid#62785PurposeLocalization: Microtubules/Kinesin, C1 Cloning Vector, Excitation:399, Emission:456DepositorInsertMCAK (KIF2C Human)
UseTagsmTagBFP2ExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
ptagBFP-LacI
Plasmid#103839PurposeFlourescent marker for lacO arraysDepositorInserttagBFP-LacI
UseTagsExpressionMammalianMutationLacI: C19T (silent, L7L), T264C (silent, A88A), C…PromoterSV2Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-Annexin-12
Plasmid#55276DepositorInsertAnnexin (A4) (ANXA4 Human)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYES-mtBFP
Plasmid#45055DepositorInsertmtBFP
UseTagsExpressionYeastMutationPromoterGALAvailable sinceMay 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-mTagBFP2
Plasmid#184940PurposeER Visualization (OSER assay) Expresses mTagBFP2 in fusion with N-terminal part of p450DepositorInsertCytERM-mTagBFP2
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only