Addgene

We narrowed to 13,020 results for: Mpl;

Showing: 401 - 440 of 13020 results

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PlayBack-CMV-EcoR1

Plasmid

#203305

Purpose

For expression of eukaryotic genes of interest with CMV promoter with EcoRI as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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PlayBack-CMV-Bgl11

Plasmid

#203306

Purpose

For expression of eukaryotic genes of interest with CMV promoter with BglII as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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PlayBack-Ef1a-Nde1

Plasmid

#203309

Purpose

For expression of eukaryotic genes of interest with Ef1a promoter with NdeI as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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PlayBack-Ef1a-Nhe1

Plasmid

#203310

Purpose

For expression of eukaryotic genes of interest with Ef1a promoter with NheI as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pDA313

Plasmid

#195214

Purpose

Expression of superfolder GFP (sfGFP) by a hybrid T7 promoter with tetO.

Depositor

Rahul Sarpeshkar

Article

Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

Insert

sfGFP with tetO and a hairpin

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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LI F-G Tet

Plasmid

#104524

Purpose

LI resistance gene module

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Tetracycline resistance gene

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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LI F-G Cml

Plasmid

#104525

Purpose

LI resistance gene module

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Chloramphenicol resistance gene

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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LI Rsc202375 Upstream

Plasmid

#104526

Purpose

LI genomic upstream module

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Rsc202375 genomic upstream region

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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LI Rsc203337 Downstream

Plasmid

#104527

Purpose

LI genomic downstream module

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Rsc203337 genomic downstream region

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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LIII 1-6 Amp RC

Plasmid

#104530

Purpose

LIII backbone vector

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Type

Empty backbone

Use

Ralstonia solanacearum transformation vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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Yes-10kb

Plasmid

#112996

Purpose

P element vector for transposon insertion of the 10Kb Drosophila Amplicon in Follicle Cells (DAFC-62D) into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Xie et al Proc Natl Acad Sci U S A. 2008 Jul 15;10

Insert

10Kb DAFC-62D amplicon

Use

P insertion vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pJK040 (pET-PurQ-A128T)

Plasmid

#73840

Purpose

Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, A128T mutant (PurQ-A128T)

Depositor

JoAnne Stubbe, T. J. Kappock

Article

Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

Insert

formylglycinamidine ribonucleotide synthetase, glutaminase subunit

Use

Tags

Expression

Bacterial

Mutation

A128T mutant

Promoter

T7

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pJK044 (pET-PurQ-wt)

Plasmid

#73839

Purpose

Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, (PurQ)

Depositor

JoAnne Stubbe, T. J. Kappock

Article

Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

Insert

formylglycinamidine ribonucleotide synthetase, glutaminase subunit

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pNG1299

Plasmid

#149709

Purpose

Template for Malachite Green Aptamer (MGA) transcription assay

Depositor

Peter Lewis

Article

Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

Insert

Triple Promoter - 12X MG Aptamer Array - Terminator construct

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mCherry-Nanobody

Plasmid

#70696

Purpose

Expresses GST-tagged anti-mCherry Nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

mCherry-Nanobody

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2016

Availability

Academic Institutions and Nonprofits only

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TagBFP2-C1-SACM1LdeltaTMD-Fis1tail

Plasmid

#220078

Purpose

SAC1 with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Blue Fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

SACM1l-Fis1tail (SACM1L Human)

Use

Tags

Expression

Mammalian

Mutation

deltaTMD

Promoter

Available since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-1-SAC1deltaTMD-Fis1tail

Plasmid

#220077

Purpose

SAC1 with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Green Fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

SACM1l-Fis1tail (SACM1L Human)

Use

Tags

Expression

Mammalian

Mutation

deltaTMD

Promoter

Available since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

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pPS22 (pET23a-Cas10/Csm/csm2H6N)

Plasmid

#70038

Purpose

Bacterial expression of the S. epidermidis Cas10·Csm complex

Depositor

Luciano Marraffini

Article

Hatoum-Aslan et al J Biol Chem. 2013 Sep 27;288(39):27888-9

Insert

Cas10/Csm complex

Use

Tags

6xHIS on N terminus of Csm2

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pFIN-NRLL-tdTOM

Plasmid

#44355

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Insert

NRLL-tdTOM

Use

Lentiviral

Tags

Expression

Mutation

Promoter

mouse neural leucine zipper (pos 3127-5587)

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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FLAG-mTIP49a

Plasmid

#15357

Depositor

Ronald Conaway, Joan Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

TATA binding protein interacting protein 49 kDa (Ruvbl1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pFIN-XOPS-tdTOM

Plasmid

#44359

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Insert

XOPS-tdTOM

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Xenopus Opsin (XOPS) (pos 3125-4501)

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pR540

Plasmid

#207885

Purpose

template for 2 gRNA amplification

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

scaffold-pU6

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-Amida

Plasmid

#15362

Depositor

Ronald Conaway, Joan Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

TCF3 (E2A) fusion partner (TFPT Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pFIN-EF1a-GFP-WPRE

Plasmid

#44352

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Insert

EF1a-GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

human elongation factor -1a (pos 2526-3969)

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pBRC169

Plasmid

#79670

Purpose

DsREDmonomer-based BiFC vector for transient expression of RC (169-225) in plants.

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Type

Empty backbone

Use

Reporter plasmid

Tags

DsRED-monomer (169–225)/RC169

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pBGN155

Plasmid

#74992

Purpose

GFP(S65T)-based BiFC vector for transient expression of sGN (1-155) in plants

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Type

Empty backbone

Use

Reporter plasmid

Tags

GFP (1–155)/GN155

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFIN-EF1a-CHER-WPRE

Plasmid

#44353

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Insert

EF1a-CHER

Use

Lentiviral

Tags

Expression

Mutation

Promoter

human elongation factor -1a (pos 2526-3969)

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pBCN155-MXMT

Plasmid

#79815

Purpose

CFP-based BiFC vector for plants (Positive control: MXMT)

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Insert

CaMXMT1

Use

Reporter plasmid

Tags

CN155/CFP (1–155)

Expression

Plant

Mutation

Promoter

CaMV 35S promoter and CaMV35S

Available since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2-C11orf83-V5

Plasmid

#65843

Purpose

Mammalian expression of C terminally V5-tagged C11orf83/UQCC3

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

UQCC3 WT (UQCC3 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

WT

Promoter

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T1 human APC-2,3 (1362–1540)

Plasmid

#132631

Purpose

expresses human APC-2,3 (1362–1540) in E. coli

Depositor

Wenqing Xu

Article

Xing et al Genes Dev. 2003 Nov 15;17(22):2753-64. d

Insert

APC-2,3 (1362–1540)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-C1-ORP5deltaTMD-FKBP

Plasmid

#220073

Purpose

expresses ORP5 with the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5.

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

OSBPL5deltaTMD (OSBPL5 Human)

Use

Tags

Expression

Mammalian

Mutation

deltaTMD

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T1 mouse Tankyrase1 ARC2-3 (308-655)

Plasmid

#132637

Purpose

expresses mouse Tankyrase1 ARC2-3 (308-655) in E. coli

Depositor

Wenqing Xu

Article

Morrone et al Proc Natl Acad Sci U S A. 2012 Jan 31;10

Insert

Tankyrase1 (308-655)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
LI F-G Gent

Plasmid

#104522

Purpose

LI resistance gene module. Please note that the plasmid contains Apramycin selection, not Gentamycin.

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Apramycin resistance gene

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBRN168-MXMT

Plasmid

#79819

Purpose

DsREDmonomer-based BiFC vector for plants (Positive control: MXMT)

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Insert

CaMXMT1

Use

Reporter plasmid

Tags

RN168/DsREDmonomer (1–168)

Expression

Plant

Mutation

Promoter

CaMV 35S promoter and CaMV35S

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pIR195

Plasmid

#64810

Purpose

TCP4 expression in yeast

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

TCP4 (TCP4 Mustard Weed)

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pIR196

Plasmid

#64811

Purpose

SPL9 expression in yeast

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

SPL9 (SPL9 Mustard Weed)

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Lourido Toxoplasma CRISPR Library V1

Pooled Library

#80636

Purpose

Plasmid sgRNA library containing ten guides against each of the 8,158 predicted Toxoplasma gondii protein coding genes.

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only
pGEX6P1-mClover3-anti-rabbit-IgG(Fc)-nanobody

Plasmid

#138129

Purpose

Expresses GST-tagged mClover3-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mClover3-anti-rabbit-IgG(Fc)-nanobody

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6P1-mCherry-anti-rabbit-IgG(Fc)-nanobody

Plasmid

#138131

Purpose

Expresses GST-tagged mCherry-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mCherry-anti-rabbit-IgG(Fc)-nanobody

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
2Bc-T MBP_BoMoC(ed)_W403A_F753A_6xH

Plasmid

#185713

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

Insert

B. mori truncated R2 RT

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Changed W403 and F753 to A

Promoter

T7

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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